BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0222000 Os07g0222000|AK107870
         (148 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os07g0222000  Similar to Alpha-amylase/trypsin inhibitor (RB...   245   7e-66
Os07g0216600  Cereal seed allergen, trypsin/alpha-amylase in...    64   5e-11

>Os07g0222000 Similar to Alpha-amylase/trypsin inhibitor (RBI) (RATI)
          Length = 148

 Score =  245 bits (626), Expect = 7e-66,   Method: Compositional matrix adjust.
 Identities = 121/148 (81%), Positives = 121/148 (81%)

Query: 1   MASKNXXXXXXXXXXXXXXXXXXXXXXXXXXXCQPGMAIPHDPLRGCRRYVLRRACGLAA 60
           MASKN                           CQPGMAIPHDPLRGCRRYVLRRACGLAA
Sbjct: 1   MASKNLLLSAAVLLSVLAIAAAAATASAATTSCQPGMAIPHDPLRGCRRYVLRRACGLAA 60

Query: 61  GGRLYDWSLKERCCQELAAVPAYCRCAALAYFMDGASEGRLLEDLPGCPRETQRGLAAML 120
           GGRLYDWSLKERCCQELAAVPAYCRCAALAYFMDGASEGRLLEDLPGCPRETQRGLAAML
Sbjct: 61  GGRLYDWSLKERCCQELAAVPAYCRCAALAYFMDGASEGRLLEDLPGCPRETQRGLAAML 120

Query: 121 TTPGECNLETIHGGPYCLELTDREMPKY 148
           TTPGECNLETIHGGPYCLELTDREMPKY
Sbjct: 121 TTPGECNLETIHGGPYCLELTDREMPKY 148



>Os07g0216600 Cereal seed allergen, trypsin/alpha-amylase inhibitor family
           protein
          Length = 158

 Score = 63.5 bits (153), Expect = 5e-11,   Method: Compositional matrix adjust.
 Identities = 45/119 (37%), Positives = 59/119 (49%), Gaps = 15/119 (12%)

Query: 33  CQPGMAIPHDPLRGCRRYVLRRACGLAAGGRLYDWS-----LKERCCQELAAVPAYCRCA 87
           C+ G A+  +P+  CR Y+ R     AAGGR+           E CC+ELAAVP  CRC 
Sbjct: 35  CRVGKAVSRNPVPSCRNYIARWC--AAAGGRMDSRKQPPREFLEPCCRELAAVPMQCRCD 92

Query: 88  ALAYFMDGA--SEGRLLEDL------PGCPRETQRGLAAMLTTPGECNLETIHGGPYCL 138
           AL+  + G    EG  +  +      PGC   T  G+A+ LT  G CNL+   G   CL
Sbjct: 93  ALSVLVRGVVTEEGDRVSGMISQHAAPGCDAATIAGMASALTDYGRCNLQHTAGSFACL 151



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.324    0.140    0.469 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,873,928
Number of extensions: 193780
Number of successful extensions: 507
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 506
Number of HSP's successfully gapped: 4
Length of query: 148
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 56
Effective length of database: 12,232,113
Effective search space: 684998328
Effective search space used: 684998328
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 151 (62.8 bits)