BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0159800 Os07g0159800|AK060475
         (152 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os07g0159800  E1 protein and Def2/Der2 allergen family protein    266   4e-72
Os03g0788200  E1 protein and Def2/Der2 allergen family protein    202   9e-53

>Os07g0159800 E1 protein and Def2/Der2 allergen family protein
          Length = 152

 Score =  266 bits (680), Expect = 4e-72,   Method: Compositional matrix adjust.
 Identities = 133/152 (87%), Positives = 133/152 (87%)

Query: 1   MATKQXXXXXXXXXXXXXXXXXXXTDVEYCNKGKKYPVKVSGVEIVPDPVARGEPATFKI 60
           MATKQ                   TDVEYCNKGKKYPVKVSGVEIVPDPVARGEPATFKI
Sbjct: 1   MATKQSLLLLLALLAAAAAVASAVTDVEYCNKGKKYPVKVSGVEIVPDPVARGEPATFKI 60

Query: 61  SASTDKTIGKGKLVIDVKYFFFYVHSETRELCDVTSCPASGDFLVAHQQTLPSYTPPGSY 120
           SASTDKTIGKGKLVIDVKYFFFYVHSETRELCDVTSCPASGDFLVAHQQTLPSYTPPGSY
Sbjct: 61  SASTDKTIGKGKLVIDVKYFFFYVHSETRELCDVTSCPASGDFLVAHQQTLPSYTPPGSY 120

Query: 121 TITMKMLGDNDEELSCISFGFSIGFAASEATI 152
           TITMKMLGDNDEELSCISFGFSIGFAASEATI
Sbjct: 121 TITMKMLGDNDEELSCISFGFSIGFAASEATI 152



>Os03g0788200 E1 protein and Def2/Der2 allergen family protein
          Length = 156

 Score =  202 bits (514), Expect = 9e-53,   Method: Compositional matrix adjust.
 Identities = 93/128 (72%), Positives = 112/128 (87%)

Query: 25  TDVEYCNKGKKYPVKVSGVEIVPDPVARGEPATFKISASTDKTIGKGKLVIDVKYFFFYV 84
           TDVEYC +G+ YPVKVSGVEIVPDPV  G+PATFKISASTDK+I KGKLVIDVKYFFF+V
Sbjct: 29  TDVEYCRQGRDYPVKVSGVEIVPDPVVSGQPATFKISASTDKSITKGKLVIDVKYFFFHV 88

Query: 85  HSETRELCDVTSCPASGDFLVAHQQTLPSYTPPGSYTITMKMLGDNDEELSCISFGFSIG 144
           HSE+  LC+ TSCP +G+F++AH+QTLPS TPPGSYT+TM++L D ++EL+CISFGFSIG
Sbjct: 89  HSESHNLCEETSCPVTGEFVLAHEQTLPSITPPGSYTLTMRLLDDGNKELTCISFGFSIG 148

Query: 145 FAASEATI 152
           F +  A I
Sbjct: 149 FISPLALI 156



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.134    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,702,592
Number of extensions: 183112
Number of successful extensions: 297
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 297
Number of HSP's successfully gapped: 2
Length of query: 152
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 60
Effective length of database: 12,232,113
Effective search space: 733926780
Effective search space used: 733926780
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 151 (62.8 bits)