BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os07g0158400 Os07g0158400|Os07g0158400
         (191 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os07g0158400  GCK domain containing protein                       172   1e-43
Os03g0647400  GCK domain containing protein                        99   2e-21

>Os07g0158400 GCK domain containing protein
          Length = 191

 Score =  172 bits (436), Expect = 1e-43,   Method: Compositional matrix adjust.
 Identities = 91/134 (67%), Positives = 91/134 (67%)

Query: 58  CGFCLFMKGGGCKEAFVAWEECVEAAGKEEGSDMVERCFEVTANLKRCMDAHADYYAPVL 117
           CGFCLFMKGGGCKEAFVAWEECVEAAGKEEGSDMVERCFEVTANLKRCMDAHADYYAPVL
Sbjct: 58  CGFCLFMKGGGCKEAFVAWEECVEAAGKEEGSDMVERCFEVTANLKRCMDAHADYYAPVL 117

Query: 118 RAEQAVNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQVEEKXXXXXXXPTTTI 177
           RAEQAVN                                    QVEEK       PTTTI
Sbjct: 118 RAEQAVNDHADAAIAADKAKEGGEKKLDAVAQEAASAADEKKQQVEEKSSSSSSSPTTTI 177

Query: 178 DERKEKEVSNKGED 191
           DERKEKEVSNKGED
Sbjct: 178 DERKEKEVSNKGED 191



>Os03g0647400 GCK domain containing protein
          Length = 196

 Score = 99.0 bits (245), Expect = 2e-21,   Method: Compositional matrix adjust.
 Identities = 45/66 (68%), Positives = 55/66 (83%), Gaps = 1/66 (1%)

Query: 58  CGFCLFMKGGGCKEAFVAWEECVEAAGKEEGSDMVERCFEVTANLKRCMDAHADYYAPVL 117
           CGFCLFMKGGGC+E FV WE+CVE A K  G D+VERC+EVTA L +CM+AHA+YY P+L
Sbjct: 75  CGFCLFMKGGGCREEFVGWEKCVEDAEKA-GDDVVERCYEVTAALHKCMEAHAEYYHPIL 133

Query: 118 RAEQAV 123
            AE+A+
Sbjct: 134 SAERAM 139



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.132    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,253,664
Number of extensions: 92641
Number of successful extensions: 203
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 201
Number of HSP's successfully gapped: 2
Length of query: 191
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 96
Effective length of database: 12,075,471
Effective search space: 1159245216
Effective search space used: 1159245216
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 153 (63.5 bits)