BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os07g0152800 Os07g0152800|AK065458
(280 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os07g0152800 Conserved hypothetical protein 224 4e-59
Os03g0786000 Conserved hypothetical protein 174 5e-44
>Os07g0152800 Conserved hypothetical protein
Length = 280
Score = 224 bits (572), Expect = 4e-59, Method: Compositional matrix adjust.
Identities = 109/115 (94%), Positives = 109/115 (94%)
Query: 166 QDPNSFGXXXXXXSVWVSFLRVMHGVVNFFGRVAFLVEQNTQAFYLFITAMLQLFDRSGM 225
QDPNSFG SVWVSFLRVMHGVVNFFGRVAFLVEQNTQAFYLFITAMLQLFDRSGM
Sbjct: 166 QDPNSFGPPAPPPSVWVSFLRVMHGVVNFFGRVAFLVEQNTQAFYLFITAMLQLFDRSGM 225
Query: 226 LYGELARFVLRMLGIRTKSKKGKVQGPDTPAFEGPAQQFIEAPKGNNSWDNVWGN 280
LYGELARFVLRMLGIRTKSKKGKVQGPDTPAFEGPAQQFIEAPKGNNSWDNVWGN
Sbjct: 226 LYGELARFVLRMLGIRTKSKKGKVQGPDTPAFEGPAQQFIEAPKGNNSWDNVWGN 280
Score = 149 bits (375), Expect = 3e-36, Method: Compositional matrix adjust.
Identities = 74/74 (100%), Positives = 74/74 (100%)
Query: 1 MAGSPPKPWERAGAEGTSGPAPFKPPSGGTTSDVVEASGTAKPGETVTATERNLSANVNN 60
MAGSPPKPWERAGAEGTSGPAPFKPPSGGTTSDVVEASGTAKPGETVTATERNLSANVNN
Sbjct: 1 MAGSPPKPWERAGAEGTSGPAPFKPPSGGTTSDVVEASGTAKPGETVTATERNLSANVNN 60
Query: 61 PVSRPMPQRPWQQT 74
PVSRPMPQRPWQQT
Sbjct: 61 PVSRPMPQRPWQQT 74
>Os03g0786000 Conserved hypothetical protein
Length = 270
Score = 174 bits (442), Expect = 5e-44, Method: Compositional matrix adjust.
Identities = 80/113 (70%), Positives = 92/113 (81%)
Query: 166 QDPNSFGXXXXXXSVWVSFLRVMHGVVNFFGRVAFLVEQNTQAFYLFITAMLQLFDRSGM 225
Q PNSFG W+SFLRVMHGVVNF GRVAFL QNTQAF++FI+A+LQL DR+GM
Sbjct: 151 QGPNSFGPPAPPPGFWMSFLRVMHGVVNFCGRVAFLFSQNTQAFHMFISALLQLCDRTGM 210
Query: 226 LYGELARFVLRMLGIRTKSKKGKVQGPDTPAFEGPAQQFIEAPKGNNSWDNVW 278
LYGELARFVLR+LGI+TK+KKG VQG +T +FEGP F EAPK NNSWD+VW
Sbjct: 211 LYGELARFVLRLLGIKTKAKKGGVQGAETSSFEGPGHHFAEAPKANNSWDSVW 263
Score = 109 bits (273), Expect = 2e-24, Method: Compositional matrix adjust.
Identities = 55/75 (73%), Positives = 65/75 (86%), Gaps = 4/75 (5%)
Query: 1 MAGS--PPKPWERAGAEGTSGPAPFKPPSGGTTSDVVEASGTAKPGETVTATERNLSANV 58
MAGS PPKPWERAG +SGPAPFKPPSGG+TSD+VEASGTAKPGE V+A E N+++NV
Sbjct: 1 MAGSNPPPKPWERAGT--SSGPAPFKPPSGGSTSDIVEASGTAKPGEVVSAAESNVASNV 58
Query: 59 NNPVSRPMPQRPWQQ 73
N+ +SRP+P RPWQQ
Sbjct: 59 NSTISRPVPPRPWQQ 73
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.316 0.133 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,201,262
Number of extensions: 265394
Number of successful extensions: 1237
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1236
Number of HSP's successfully gapped: 4
Length of query: 280
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 180
Effective length of database: 11,814,401
Effective search space: 2126592180
Effective search space used: 2126592180
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 155 (64.3 bits)