BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os06g0699200 Os06g0699200|J100035N11
         (141 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os06g0699200  Conserved hypothetical protein                      295   5e-81
Os09g0533300  Phosphoesterase At2g46880 family protein            157   2e-39

>Os06g0699200 Conserved hypothetical protein
          Length = 141

 Score =  295 bits (756), Expect = 5e-81,   Method: Compositional matrix adjust.
 Identities = 141/141 (100%), Positives = 141/141 (100%)

Query: 1   QQNLHAPAFAFFHIPIPEVRGLWYTGFKGQYQEGVACSTVNSGVLGTLTSMGDVKAVFLG 60
           QQNLHAPAFAFFHIPIPEVRGLWYTGFKGQYQEGVACSTVNSGVLGTLTSMGDVKAVFLG
Sbjct: 1   QQNLHAPAFAFFHIPIPEVRGLWYTGFKGQYQEGVACSTVNSGVLGTLTSMGDVKAVFLG 60

Query: 61  HDHLNDFCGDLNGIWFCYGGGFGYHAYGRPHWPRRARVIHTELKKGQKSLVEVESIHTWK 120
           HDHLNDFCGDLNGIWFCYGGGFGYHAYGRPHWPRRARVIHTELKKGQKSLVEVESIHTWK
Sbjct: 61  HDHLNDFCGDLNGIWFCYGGGFGYHAYGRPHWPRRARVIHTELKKGQKSLVEVESIHTWK 120

Query: 121 LLDDEKLTKIDEQVLWRHSSI 141
           LLDDEKLTKIDEQVLWRHSSI
Sbjct: 121 LLDDEKLTKIDEQVLWRHSSI 141



>Os09g0533300 Phosphoesterase At2g46880 family protein
          Length = 398

 Score =  157 bits (398), Expect = 2e-39,   Method: Compositional matrix adjust.
 Identities = 72/141 (51%), Positives = 90/141 (63%), Gaps = 1/141 (0%)

Query: 1   QQNLHAPAFAFFHIPIPEVRGLWYTGFKGQYQEGVACSTVNSGVLGTLTSMGDVKAVFLG 60
           +Q   AP   +FHIP+PE      + F G  QEG++  ++NSG   ++   GDVKA F+G
Sbjct: 245 KQKAAAPGLVYFHIPLPEFSSFTSSNFTGVKQEGISSPSINSGFFASMVEAGDVKAAFIG 304

Query: 61  HDHLNDFCGDLNGIWFCYGGGFGYHAYGRPHWPRRARVIHTELKKGQKSLVE-VESIHTW 119
           HDH+NDFCG LNGI  CY GGFGYHAYG+  W RRARV+  +L+K        V+SI TW
Sbjct: 305 HDHVNDFCGKLNGIQLCYAGGFGYHAYGKAGWSRRARVVSVQLEKSDGGEWRGVKSIKTW 364

Query: 120 KLLDDEKLTKIDEQVLWRHSS 140
           K LDD  LT ID +VLW   S
Sbjct: 365 KRLDDPHLTTIDSEVLWNRGS 385



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.323    0.141    0.473 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,500,250
Number of extensions: 229233
Number of successful extensions: 468
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 467
Number of HSP's successfully gapped: 2
Length of query: 141
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 50
Effective length of database: 12,284,327
Effective search space: 614216350
Effective search space used: 614216350
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 151 (62.8 bits)