BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os06g0524500 Os06g0524500|AK066466
         (114 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os06g0524500  Conserved hypothetical protein                      117   2e-27
Os06g0525000  Conserved hypothetical protein                       95   1e-20

>Os06g0524500 Conserved hypothetical protein
          Length = 114

 Score =  117 bits (292), Expect = 2e-27,   Method: Compositional matrix adjust.
 Identities = 65/114 (57%), Positives = 65/114 (57%)

Query: 1   MASQEKXXXXXXXXXXXXXXEAVPRVRRFXXXXXXXXXXXXXXXXXXXXXXXXXTLREQW 60
           MASQEK              EAVPRVRRF                         TLREQW
Sbjct: 1   MASQEKPTPPPPPPPHPPAPEAVPRVRRFGGGPPGGGGGGGYPNPPDAAIPDAATLREQW 60

Query: 61  RFAVRQYSRWYSHAWGTAIXXXXXXXXXXWLVKGSNPLPSRAEHHASNAKEEEG 114
           RFAVRQYSRWYSHAWGTAI          WLVKGSNPLPSRAEHHASNAKEEEG
Sbjct: 61  RFAVRQYSRWYSHAWGTAILAGAAFFGLGWLVKGSNPLPSRAEHHASNAKEEEG 114



>Os06g0525000 Conserved hypothetical protein
          Length = 191

 Score = 94.7 bits (234), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 46/58 (79%), Positives = 46/58 (79%)

Query: 55  TLREQWRFAVRQYSRWYSHAWGTAIXXXXXXXXXXWLVKGSNPLPSRAEHHASNAKEE 112
           TLREQW FAVRQYSRWYSHAWGTAI          WLVKGSNPLPSRAE HASNAKEE
Sbjct: 101 TLREQWLFAVRQYSRWYSHAWGTAILAGAAFFGLGWLVKGSNPLPSRAEPHASNAKEE 158



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.126    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,238,940
Number of extensions: 52689
Number of successful extensions: 66
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 65
Number of HSP's successfully gapped: 2
Length of query: 114
Length of database: 17,035,801
Length adjustment: 81
Effective length of query: 33
Effective length of database: 12,806,467
Effective search space: 422613411
Effective search space used: 422613411
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)