BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os06g0297600 Os06g0297600|AK067425
         (95 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os06g0297600  Protein of unknown function DUF250 domain cont...   193   2e-50
Os02g0724500  Protein of unknown function DUF250 domain cont...   119   7e-28

>Os06g0297600 Protein of unknown function DUF250 domain containing protein
          Length = 95

 Score =  193 bits (490), Expect = 2e-50,   Method: Compositional matrix adjust.
 Identities = 95/95 (100%), Positives = 95/95 (100%)

Query: 1  CSATSALAHVVLGQFKTIVIMLSSYLVFNSDPGFTSLCGAIIALGGMSVYTYLGLKESAS 60
          CSATSALAHVVLGQFKTIVIMLSSYLVFNSDPGFTSLCGAIIALGGMSVYTYLGLKESAS
Sbjct: 1  CSATSALAHVVLGQFKTIVIMLSSYLVFNSDPGFTSLCGAIIALGGMSVYTYLGLKESAS 60

Query: 61 GGKRAPSTSRQNSHLLKSKVIVDGEKPETRPIDSV 95
          GGKRAPSTSRQNSHLLKSKVIVDGEKPETRPIDSV
Sbjct: 61 GGKRAPSTSRQNSHLLKSKVIVDGEKPETRPIDSV 95



>Os02g0724500 Protein of unknown function DUF250 domain containing protein
          Length = 371

 Score =  119 bits (297), Expect = 7e-28,   Method: Compositional matrix adjust.
 Identities = 62/94 (65%), Positives = 70/94 (74%), Gaps = 7/94 (7%)

Query: 2   SATSALAHVVLGQFKTIVIMLSSYLVFNSDPGFTSLCGAIIALGGMSVYTYLGLKESASG 61
           +ATSAL+HVVLGQFKTIVIMLS YL+F+SDPG TS+CGAI+ALGGMSVYTYLGLKES + 
Sbjct: 285 AATSALSHVVLGQFKTIVIMLSGYLIFSSDPGITSICGAIVALGGMSVYTYLGLKESTTT 344

Query: 62  GKRAPSTSRQNSHLLKSKVIVDGEKPETRPIDSV 95
           GK+ P          K K   DGEKP     DSV
Sbjct: 345 GKKPPLAQ-------KPKAAGDGEKPGLEHEDSV 371



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.132    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,166,326
Number of extensions: 114062
Number of successful extensions: 223
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 222
Number of HSP's successfully gapped: 2
Length of query: 95
Length of database: 17,035,801
Length adjustment: 64
Effective length of query: 31
Effective length of database: 13,694,105
Effective search space: 424517255
Effective search space used: 424517255
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 149 (62.0 bits)