BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os06g0196300 Os06g0196300|AK059845
         (217 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os06g0196300  Similar to Peroxiredoxin Q (Fragment)               357   4e-99
Os02g0537700  Similar to 2-cys peroxiredoxin BAS1, chloropla...    77   1e-14
AK059961                                                           67   1e-11

>Os06g0196300 Similar to Peroxiredoxin Q (Fragment)
          Length = 217

 Score =  357 bits (915), Expect = 4e-99,   Method: Compositional matrix adjust.
 Identities = 177/217 (81%), Positives = 177/217 (81%)

Query: 1   MAFAVSTACXXXXXXXXXXXXXXXXXXXXXCTPSTAAFRRGXXXXXXXXXXXXXXXXXXX 60
           MAFAVSTAC                     CTPSTAAFRRG                   
Sbjct: 1   MAFAVSTACRPSLLLPPRQRSSPPRPRPLLCTPSTAAFRRGALSATTTPTPARAALPSTT 60

Query: 61  GRNRIVCGKVSKGSAAPNFTLRDQDGRAVSLSKFKGRPVVVYFYPADETPGCTKQACAFR 120
           GRNRIVCGKVSKGSAAPNFTLRDQDGRAVSLSKFKGRPVVVYFYPADETPGCTKQACAFR
Sbjct: 61  GRNRIVCGKVSKGSAAPNFTLRDQDGRAVSLSKFKGRPVVVYFYPADETPGCTKQACAFR 120

Query: 121 DSYEKFKKAGAEVIGISGDDAASHKEFKKKYKLPFTLLSDEGNKVRKEWGVPADLFGTLP 180
           DSYEKFKKAGAEVIGISGDDAASHKEFKKKYKLPFTLLSDEGNKVRKEWGVPADLFGTLP
Sbjct: 121 DSYEKFKKAGAEVIGISGDDAASHKEFKKKYKLPFTLLSDEGNKVRKEWGVPADLFGTLP 180

Query: 181 GRQTYVLDKNGVVQYIYNNQFQPEKHIGETLKILQSL 217
           GRQTYVLDKNGVVQYIYNNQFQPEKHIGETLKILQSL
Sbjct: 181 GRQTYVLDKNGVVQYIYNNQFQPEKHIGETLKILQSL 217



>Os02g0537700 Similar to 2-cys peroxiredoxin BAS1, chloroplast precursor (EC
           1.11.1.15) (Thiol- specific antioxidant protein)
           (Fragment)
          Length = 261

 Score = 76.6 bits (187), Expect = 1e-14,   Method: Compositional matrix adjust.
 Identities = 49/156 (31%), Positives = 83/156 (53%), Gaps = 12/156 (7%)

Query: 73  GSAAPNF---TLRDQDGRAVSLSKFKGRP-VVVYFYPADETPGCTKQACAFRDSYEKFKK 128
           G+ AP+F    + DQ+   V LS + G+  V+++FYP D T  C  +  AF D Y++F+K
Sbjct: 71  GNKAPDFDAEAVFDQEFINVKLSDYIGKKYVILFFYPLDFTFVCPTEITAFSDRYDEFEK 130

Query: 129 AGAEVIGISGDDAASHKEFKKKYK-------LPFTLLSDEGNKVRKEWGVPADLFGTLPG 181
              E++G+S D   SH  + +  +       L + L+SD    + K +GV     G +  
Sbjct: 131 LNTEILGVSIDSVFSHLAWVQTDRKSGGLGDLKYPLISDVTKSISKSFGVLIPDQG-IAL 189

Query: 182 RQTYVLDKNGVVQYIYNNQFQPEKHIGETLKILQSL 217
           R  +++DK GV+Q+   N     + + ET++ LQ+L
Sbjct: 190 RGLFIIDKEGVIQHSTINNLAIGRSVDETMRTLQAL 225



>AK059961 
          Length = 197

 Score = 67.0 bits (162), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 46/158 (29%), Positives = 78/158 (49%), Gaps = 17/158 (10%)

Query: 73  GSAAPNFT----LRDQDGRAVSLSKFKGRPVVVYFYPADETPGCTKQACAFRDSYEKFKK 128
           G  AP F+    + D   + VSLS + G+ +V++FYP D T  C  +   + +  E+F  
Sbjct: 6   GHKAPAFSTEALMPDGSFKKVSLSDYAGKWLVLFFYPLDFTFVCPTEIIQYSERVEEFNT 65

Query: 129 AGAEVIGISGDDAASHKEFKKK---------YKLPFTLLSDEGNKVRKEWGVPADLFGTL 179
            GAEV+  S D   SH  + K+          K+P  +L D   +V K +GV  +  G +
Sbjct: 66  IGAEVVAASVDSVFSHLAWTKQPRKAGGLGPMKIP--ILGDITKEVSKAYGVLNE--GGV 121

Query: 180 PGRQTYVLDKNGVVQYIYNNQFQPEKHIGETLKILQSL 217
             R  +++D    ++ I  N     +++ ETL++LQ+ 
Sbjct: 122 AFRGVFIIDPEQNIRQITINDLPVGRNVDETLRLLQAF 159



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.135    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,110,921
Number of extensions: 251051
Number of successful extensions: 493
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 489
Number of HSP's successfully gapped: 3
Length of query: 217
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 120
Effective length of database: 11,971,043
Effective search space: 1436525160
Effective search space used: 1436525160
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 154 (63.9 bits)