BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os06g0188100 Os06g0188100|AK069205
         (323 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os06g0188100  Protein of unknown function DUF250 domain cont...   596   e-171
Os02g0614100  Protein of unknown function DUF250 domain cont...   112   4e-25

>Os06g0188100 Protein of unknown function DUF250 domain containing protein
          Length = 323

 Score =  596 bits (1537), Expect = e-171,   Method: Compositional matrix adjust.
 Identities = 298/323 (92%), Positives = 298/323 (92%)

Query: 1   MGAETGEPXXXXXXXXXXXYGIASMAMVFVNKAILMQYAHSMXXXXXXXXXXXXXXHFGQ 60
           MGAETGEP           YGIASMAMVFVNKAILMQYAHSM              HFGQ
Sbjct: 1   MGAETGEPSSFLSLSAAFSYGIASMAMVFVNKAILMQYAHSMTLLTLQQIATALIIHFGQ 60

Query: 61  ILGVSKRKDFSMATGRKLLPLSIFYNANVAFALASLKGVNIPMYIAIKRLTPLAVLVAGC 120
           ILGVSKRKDFSMATGRKLLPLSIFYNANVAFALASLKGVNIPMYIAIKRLTPLAVLVAGC
Sbjct: 61  ILGVSKRKDFSMATGRKLLPLSIFYNANVAFALASLKGVNIPMYIAIKRLTPLAVLVAGC 120

Query: 121 LRGKGKPPTQVSLSVICTAAGVLIAALGDFSFDLYGYSMALTSVFFQTMYLILVEKSGAE 180
           LRGKGKPPTQVSLSVICTAAGVLIAALGDFSFDLYGYSMALTSVFFQTMYLILVEKSGAE
Sbjct: 121 LRGKGKPPTQVSLSVICTAAGVLIAALGDFSFDLYGYSMALTSVFFQTMYLILVEKSGAE 180

Query: 181 DGLSSVELMFYNSVLSLPFLFFIIIATGEFPYSLSVLSEKTASLTFSAILLVSLVMGIVL 240
           DGLSSVELMFYNSVLSLPFLFFIIIATGEFPYSLSVLSEKTASLTFSAILLVSLVMGIVL
Sbjct: 181 DGLSSVELMFYNSVLSLPFLFFIIIATGEFPYSLSVLSEKTASLTFSAILLVSLVMGIVL 240

Query: 241 NFTMFWCTIVNSALTTTIVGVLKGVGSTTLGFVLLGGVEVHTLNVTGLVINTFGGVWYSY 300
           NFTMFWCTIVNSALTTTIVGVLKGVGSTTLGFVLLGGVEVHTLNVTGLVINTFGGVWYSY
Sbjct: 241 NFTMFWCTIVNSALTTTIVGVLKGVGSTTLGFVLLGGVEVHTLNVTGLVINTFGGVWYSY 300

Query: 301 AKYMQKRKMPKRIAPDVEAHPHK 323
           AKYMQKRKMPKRIAPDVEAHPHK
Sbjct: 301 AKYMQKRKMPKRIAPDVEAHPHK 323



>Os02g0614100 Protein of unknown function DUF250 domain containing protein
          Length = 346

 Score =  112 bits (280), Expect = 4e-25,   Method: Compositional matrix adjust.
 Identities = 79/235 (33%), Positives = 120/235 (51%), Gaps = 18/235 (7%)

Query: 80  PLSIFYNANVAFALASLKGVNIPMYIAIKRLTPLAVLVAGCLRGKGK--PPTQVSLSVIC 137
           PLS+ Y   +  ++ S++GVN+PMY  ++R T +  +       K K  PP   S+++I 
Sbjct: 122 PLSLAYLLYMLASMESVRGVNVPMYTTLRRTTVVFTMTMEYFLAKQKHTPPIIGSVALI- 180

Query: 138 TAAGVLIAALGDFSFDLYGYSMALTSVFFQTMYLILVEKSGAEDGLSSVELMFYNSVLSL 197
              G  IA   D SFD  GY++   +     +YL  + + G   GL+S  LM+ N ++  
Sbjct: 181 -VFGAFIAGARDLSFDARGYAIVFVANITTAVYLATINRIGKSSGLNSFGLMWCNGLVCG 239

Query: 198 PFLFFIIIATG------EFPYSLSVLSEKTASLTFSAILLVSLVMGIVLNFTMFWCTIVN 251
           P + F+    G      EFPY  S          F A+LL S ++  +LN+T+FW TI+N
Sbjct: 240 PSVLFLTYIQGDLKKAIEFPYLYSP--------GFQAVLLFSCMLAFLLNYTIFWNTILN 291

Query: 252 SALTTTIVGVLKGVGSTTLGFVLLGGVEVHTLNVTGLVINTFGGVWYSYAKYMQK 306
           SALT ++ G LK   +  +G+VL GG+    LNV G  +   G   Y+Y K   K
Sbjct: 292 SALTQSMCGNLKDFFTVGIGWVLFGGLPFDLLNVIGQGLGFLGSGLYAYCKIKGK 346



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.138    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,317,346
Number of extensions: 287128
Number of successful extensions: 977
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 988
Number of HSP's successfully gapped: 5
Length of query: 323
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 222
Effective length of database: 11,762,187
Effective search space: 2611205514
Effective search space used: 2611205514
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 156 (64.7 bits)