BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os06g0153100 Os06g0153100|J100078M15
         (84 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os06g0153100  Protein of unknown function DUF581 family protein   145   8e-36
Os06g0125200  Protein of unknown function DUF581 family protein   107   2e-24

>Os06g0153100 Protein of unknown function DUF581 family protein
          Length = 84

 Score =  145 bits (365), Expect = 8e-36,   Method: Compositional matrix adjust.
 Identities = 72/84 (85%), Positives = 72/84 (85%)

Query: 1  MYKGNVAFCCDDCRQDKMDMDSALAAVKRRHRTLQXXXXXXXXXXXXAPAEAQCADNEAG 60
          MYKGNVAFCCDDCRQDKMDMDSALAAVKRRHRTLQ            APAEAQCADNEAG
Sbjct: 1  MYKGNVAFCCDDCRQDKMDMDSALAAVKRRHRTLQRSSRDRSSSSSSAPAEAQCADNEAG 60

Query: 61 LFAVIPRRPTVSDLTTHAAPAVSG 84
          LFAVIPRRPTVSDLTTHAAPAVSG
Sbjct: 61 LFAVIPRRPTVSDLTTHAAPAVSG 84



>Os06g0125200 Protein of unknown function DUF581 family protein
          Length = 144

 Score =  107 bits (266), Expect = 2e-24,   Method: Compositional matrix adjust.
 Identities = 61/84 (72%), Positives = 63/84 (75%), Gaps = 1/84 (1%)

Query: 1   MYKGNVAFCCDDCRQDKMDMDSALAAVKRRHRTLQXXXXXXXXXXXXAPAEAQCADNEAG 60
           MYKGN AFC DDCRQD+MDMDSALAAVKRRHRTLQ              A    A NEAG
Sbjct: 60  MYKGNAAFCSDDCRQDQMDMDSALAAVKRRHRTLQRSRDMSSSSSPAPAAAQC-AANEAG 118

Query: 61  LFAVIPRRPTVSDLTTHAAPAVSG 84
           LFAVIPRRPTV+DLTTHAAPAVSG
Sbjct: 119 LFAVIPRRPTVADLTTHAAPAVSG 142



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.130    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,469,237
Number of extensions: 62148
Number of successful extensions: 106
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 104
Number of HSP's successfully gapped: 3
Length of query: 84
Length of database: 17,035,801
Length adjustment: 54
Effective length of query: 30
Effective length of database: 14,216,245
Effective search space: 426487350
Effective search space used: 426487350
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 149 (62.0 bits)