BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os06g0133400 Os06g0133400|009-145-C03
(91 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os06g0133400 Conserved hypothetical protein 159 4e-40
Os06g0133500 Conserved hypothetical protein 75 1e-14
>Os06g0133400 Conserved hypothetical protein
Length = 91
Score = 159 bits (403), Expect = 4e-40, Method: Compositional matrix adjust.
Identities = 79/91 (86%), Positives = 79/91 (86%)
Query: 1 MDKILAFSILSSSPAEIAAPGYYTRFSWRTTSXXXXXXXXXXXXPTRQQEGEKQQAGRSS 60
MDKILAFSILSSSPAEIAAPGYYTRFSWRTTS PTRQQEGEKQQAGRSS
Sbjct: 1 MDKILAFSILSSSPAEIAAPGYYTRFSWRTTSAGKQQKAAAEKAPTRQQEGEKQQAGRSS 60
Query: 61 PAAAAERKPVARPRFAPEFDGINCFETIVPF 91
PAAAAERKPVARPRFAPEFDGINCFETIVPF
Sbjct: 61 PAAAAERKPVARPRFAPEFDGINCFETIVPF 91
>Os06g0133500 Conserved hypothetical protein
Length = 90
Score = 75.1 bits (183), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 48/95 (50%), Positives = 57/95 (60%), Gaps = 13/95 (13%)
Query: 1 MDKILAFSILSSSPAEIAAPGYYTRFSWRTTSXXXXXXXXXXXXPTRQQEGEKQQAGR-S 59
MDK+LAFSILS+SPA+IAA GY+ R SWR + R+Q E+QQ G S
Sbjct: 1 MDKVLAFSILSASPADIAAGGYWARLSWRRKA-------DDQAVDGRRQPSEQQQRGEGS 53
Query: 60 SPA-----AAAERKPVARPRFAPEFDGINCFETIV 89
SP+ P PRFAPEFDGI+CFETIV
Sbjct: 54 SPSQREERRRPREAPPLPPRFAPEFDGIDCFETIV 88
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.317 0.130 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,027,531
Number of extensions: 98745
Number of successful extensions: 442
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 441
Number of HSP's successfully gapped: 2
Length of query: 91
Length of database: 17,035,801
Length adjustment: 61
Effective length of query: 30
Effective length of database: 13,850,747
Effective search space: 415522410
Effective search space used: 415522410
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 149 (62.0 bits)