BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0547100 Os05g0547100|AK121657
(145 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os05g0547100 Conserved hypothetical protein 301 1e-82
Os03g0750900 Conserved hypothetical protein 165 1e-41
Os07g0118500 80 6e-16
>Os05g0547100 Conserved hypothetical protein
Length = 145
Score = 301 bits (771), Expect = 1e-82, Method: Compositional matrix adjust.
Identities = 145/145 (100%), Positives = 145/145 (100%)
Query: 1 MDKKPARNLTACLAAIAGAVVNNSNGGKLPGLSRKLFQKAPSEHKKNALTEVKSGGNTRA 60
MDKKPARNLTACLAAIAGAVVNNSNGGKLPGLSRKLFQKAPSEHKKNALTEVKSGGNTRA
Sbjct: 1 MDKKPARNLTACLAAIAGAVVNNSNGGKLPGLSRKLFQKAPSEHKKNALTEVKSGGNTRA 60
Query: 61 LAMVLRGEWVDEIAALRLQFEQKDIEVERLKDLCLWQREEIWTLKGAVFFPATQPDHNLT 120
LAMVLRGEWVDEIAALRLQFEQKDIEVERLKDLCLWQREEIWTLKGAVFFPATQPDHNLT
Sbjct: 61 LAMVLRGEWVDEIAALRLQFEQKDIEVERLKDLCLWQREEIWTLKGAVFFPATQPDHNLT 120
Query: 121 GQIQYLAEKIAVVKTKKHMPRSCMF 145
GQIQYLAEKIAVVKTKKHMPRSCMF
Sbjct: 121 GQIQYLAEKIAVVKTKKHMPRSCMF 145
>Os03g0750900 Conserved hypothetical protein
Length = 219
Score = 165 bits (417), Expect = 1e-41, Method: Compositional matrix adjust.
Identities = 98/148 (66%), Positives = 104/148 (70%), Gaps = 17/148 (11%)
Query: 1 MDKKPARNLTACLAAIAGAVVNNSNGGKLPGLSRKLFQKAPSEHKKNALTEVKSGGNTRA 60
MDKKPA+NL AA A A N +GGKLPGLSRKLFQK SE KK ALTEVK+GGNTR
Sbjct: 68 MDKKPAKNLPVPPAAAAAAAAKNGSGGKLPGLSRKLFQKGSSEPKKKALTEVKNGGNTRT 127
Query: 61 LAMVLRG---------EWVDEIAALRLQFEQKDIE-VERLKDLCLWQREEIWTLKGAVFF 110
LAMVLR E DEIAALRLQ EQKD E VERLKDLCL QREEI TLK AV F
Sbjct: 128 LAMVLRSERELLTQSKEQEDEIAALRLQLEQKDTEQVERLKDLCLRQREEIRTLKDAVLF 187
Query: 111 PATQPDHN-------LTGQIQYLAEKIA 131
P TQPD + LTGQIQ LAE++A
Sbjct: 188 PDTQPDRHLRDEISTLTGQIQCLAEELA 215
>Os07g0118500
Length = 84
Score = 79.7 bits (195), Expect = 6e-16, Method: Compositional matrix adjust.
Identities = 39/49 (79%), Positives = 42/49 (85%)
Query: 38 QKAPSEHKKNALTEVKSGGNTRALAMVLRGEWVDEIAALRLQFEQKDIE 86
+KA SEHKKNALT+VKS GNTR +MVLR EW DEIA LRLQFEQKDIE
Sbjct: 19 RKALSEHKKNALTKVKSSGNTREHSMVLRDEWEDEIATLRLQFEQKDIE 67
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.319 0.132 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,141,993
Number of extensions: 192773
Number of successful extensions: 425
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 422
Number of HSP's successfully gapped: 3
Length of query: 145
Length of database: 17,035,801
Length adjustment: 91
Effective length of query: 54
Effective length of database: 12,284,327
Effective search space: 663353658
Effective search space used: 663353658
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 151 (62.8 bits)