BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0543000 Os05g0543000|AK107100
         (206 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0543000  Plant invertase/pectin methylesterase inhibito...   322   1e-88
Os01g0704000  Pectinesterase inhibitor domain containing pro...    84   5e-17

>Os05g0543000 Plant invertase/pectin methylesterase inhibitor domain containing
           protein
          Length = 206

 Score =  322 bits (825), Expect = 1e-88,   Method: Compositional matrix adjust.
 Identities = 162/181 (89%), Positives = 162/181 (89%)

Query: 26  GPIDVAPTNLITNPLGAIIDNGRKITGAVVDECAWTCDHVAAGNKKMCNTLRKLPGVSSP 85
           GPIDVAPTNLITNPLGAIIDNGRKITGAVVDECAWTCDHVAAGNKKMCNTLRKLPGVSSP
Sbjct: 26  GPIDVAPTNLITNPLGAIIDNGRKITGAVVDECAWTCDHVAAGNKKMCNTLRKLPGVSSP 85

Query: 86  KELLTAAVKLSMXXXXXXXXXXXXXXXXXXXGTPMESILDTCKEGYDSTVSALQEVQRCI 145
           KELLTAAVKLSM                   GTPMESILDTCKEGYDSTVSALQEVQRCI
Sbjct: 86  KELLTAAVKLSMRKAKAARARFEAAARAAEKGTPMESILDTCKEGYDSTVSALQEVQRCI 145

Query: 146 DANDSKASLITKMSAATTFTGDCGNAYEERELEPSLALKATKNNVNRVVTGALAIAAKLK 205
           DANDSKASLITKMSAATTFTGDCGNAYEERELEPSLALKATKNNVNRVVTGALAIAAKLK
Sbjct: 146 DANDSKASLITKMSAATTFTGDCGNAYEERELEPSLALKATKNNVNRVVTGALAIAAKLK 205

Query: 206 L 206
           L
Sbjct: 206 L 206



>Os01g0704000 Pectinesterase inhibitor domain containing protein
          Length = 203

 Score = 84.3 bits (207), Expect = 5e-17,   Method: Compositional matrix adjust.
 Identities = 61/189 (32%), Positives = 93/189 (49%), Gaps = 18/189 (9%)

Query: 26  GPIDVAPTNLITNPLGAIIDNG----RKITGAVVDECAWTCDHVAAGNKKMCNTLRKLPG 81
           G +D  P +  T P   +I       R++ G  +   A  C  V    K +C TL  LPG
Sbjct: 22  GVVDARPVH--TGPYHVVIRTAAKPSRRLIG--LGGNAAICQEV--HYKALCGTLTTLPG 75

Query: 82  VSSPKELLTAAVKLSMXXXXXXXXXXX---XXXXXXXXGTPMESILDTCKEGYDSTVSAL 138
           V +P++LL AA++++                       GT M S LDTCK  Y S   AL
Sbjct: 76  VMTPQQLLDAALRVAESKAMMAEKRLADVMKSRAVKAEGTSMSSTLDTCKGAYSSLADAL 135

Query: 139 QEVQRCIDANDSKASLITKMSAATTFTGDCGNAYEERELEPSLA--LKATKNNVNRVVTG 196
           Q+ +  I +  S   L+T++S+A+TF+ DCG A++E    P L   +   + +VNR+V+ 
Sbjct: 136 QKARDTIKSGGSHDDLMTELSSASTFSTDCGEAFDEF---PDLTSPIPGAQRHVNRLVSN 192

Query: 197 ALAIAAKLK 205
            L +AA +K
Sbjct: 193 CLDLAATIK 201



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.314    0.128    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,984,464
Number of extensions: 158392
Number of successful extensions: 281
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 279
Number of HSP's successfully gapped: 2
Length of query: 206
Length of database: 17,035,801
Length adjustment: 96
Effective length of query: 110
Effective length of database: 12,023,257
Effective search space: 1322558270
Effective search space used: 1322558270
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 153 (63.5 bits)