BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0536400 Os05g0536400|AK121765
         (464 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0536400  Similar to Delta-1-pyrroline-5-carboxylate deh...   927   0.0  
Os08g0440800  Glyceraldehyde-3-phosphate dehydrogenase             68   1e-11
AK062559                                                           68   1e-11

>Os05g0536400 Similar to Delta-1-pyrroline-5-carboxylate dehydrogenase (EC
           1.5.1.12)
          Length = 464

 Score =  927 bits (2397), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 447/447 (100%), Positives = 447/447 (100%)

Query: 18  GPAALASRWMHTPPFATVSPQEISGSSPAEVQNFVQGSWTTSGNWNWLVDPLNGEKFIKV 77
           GPAALASRWMHTPPFATVSPQEISGSSPAEVQNFVQGSWTTSGNWNWLVDPLNGEKFIKV
Sbjct: 18  GPAALASRWMHTPPFATVSPQEISGSSPAEVQNFVQGSWTTSGNWNWLVDPLNGEKFIKV 77

Query: 78  AEVQEAEIKPFVESLSNCPKHGLHNPLKAPERYLMYGDISAKAANMLGQPVVSDFFAKLI 137
           AEVQEAEIKPFVESLSNCPKHGLHNPLKAPERYLMYGDISAKAANMLGQPVVSDFFAKLI
Sbjct: 78  AEVQEAEIKPFVESLSNCPKHGLHNPLKAPERYLMYGDISAKAANMLGQPVVSDFFAKLI 137

Query: 138 QRVSPKSYQQALAEVQVSQKFLENFCGDQVRFLARSFAVPGNHLGQSSNGYRWPYGPVAI 197
           QRVSPKSYQQALAEVQVSQKFLENFCGDQVRFLARSFAVPGNHLGQSSNGYRWPYGPVAI
Sbjct: 138 QRVSPKSYQQALAEVQVSQKFLENFCGDQVRFLARSFAVPGNHLGQSSNGYRWPYGPVAI 197

Query: 198 ITPFNFPLEIPLLQLMGALYMGNKPVLKVDSKVSIVMDQMLRLLHACGMPAEDVDFINSD 257
           ITPFNFPLEIPLLQLMGALYMGNKPVLKVDSKVSIVMDQMLRLLHACGMPAEDVDFINSD
Sbjct: 198 ITPFNFPLEIPLLQLMGALYMGNKPVLKVDSKVSIVMDQMLRLLHACGMPAEDVDFINSD 257

Query: 258 GITMNKLLLEANPKMTLFTGSSRIAEKLAADLKGKIKLEDAGFDWKILGPDVQEVDYIAW 317
           GITMNKLLLEANPKMTLFTGSSRIAEKLAADLKGKIKLEDAGFDWKILGPDVQEVDYIAW
Sbjct: 258 GITMNKLLLEANPKMTLFTGSSRIAEKLAADLKGKIKLEDAGFDWKILGPDVQEVDYIAW 317

Query: 318 VCDQDAYACSGQKCSAQSILFMHKNWSSSGLLDKMKSLSERRKLEDLTIGPVLTVTTSSM 377
           VCDQDAYACSGQKCSAQSILFMHKNWSSSGLLDKMKSLSERRKLEDLTIGPVLTVTTSSM
Sbjct: 318 VCDQDAYACSGQKCSAQSILFMHKNWSSSGLLDKMKSLSERRKLEDLTIGPVLTVTTSSM 377

Query: 378 IEHMKNLLKIPGSKVLFGGEPLENHSIPEIYGAFKPTAVFVPLSEILKSGNFELVTREIF 437
           IEHMKNLLKIPGSKVLFGGEPLENHSIPEIYGAFKPTAVFVPLSEILKSGNFELVTREIF
Sbjct: 378 IEHMKNLLKIPGSKVLFGGEPLENHSIPEIYGAFKPTAVFVPLSEILKSGNFELVTREIF 437

Query: 438 GPFQVVTEYSDDELELVLEACERMNAI 464
           GPFQVVTEYSDDELELVLEACERMNAI
Sbjct: 438 GPFQVVTEYSDDELELVLEACERMNAI 464



>Os08g0440800 Glyceraldehyde-3-phosphate dehydrogenase
          Length = 499

 Score = 68.2 bits (165), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 80/349 (22%), Positives = 151/349 (43%), Gaps = 34/349 (9%)

Query: 51  FVQGSW--TTSGNWNWLVDPLNGEKFIKVAEVQEAEIKPFVESLSNCPKHGLHNPLKAPE 108
           +  G W  + SG    +V+P       +V    + E+   +E+     K     PL    
Sbjct: 22  YADGEWRVSASGKSVAIVNPTTRLTQYRVQACTQEEVNKVMETAKVAQKAWARTPLWKRA 81

Query: 109 RYLMYGDISAKAANMLGQPVVSDFFAKLIQRVSPKSYQQALAEVQVSQKFLENFCGDQVR 168
             L       KAA +L +       A+ + +   K  + A++EV  S   +     + VR
Sbjct: 82  ELLH------KAAAILKEHKTP--IAECLVKEIAKPAKDAISEVVRSGDLVSYTAEEGVR 133

Query: 169 FLAR-----SFAVPGNHLGQSSNGYRWPYGPVAIITPFNFPLEIPLLQLMGALYMGNKPV 223
            L       S + PGN   +     + P G V  I PFN+P+ + + ++  AL  GN  V
Sbjct: 134 ILGEGKLLVSDSFPGNERNKYCLSSKVPLGVVLAIPPFNYPVNLAVSKIGPALIAGNALV 193

Query: 224 LKVDSKVSIVMDQMLRLLHACGMPAEDVDFINSDGITMNKLL-LEANPKMTLFTGS-SRI 281
           LK  ++ ++    M+   H  G P   ++ +   G  +   L +        FTG  + I
Sbjct: 194 LKPPTQGAVAALHMVHCFHLAGFPKGLINCVTGKGSEIGDFLTMHPGVNCISFTGGDTGI 253

Query: 282 AEKLAADLKGKIKLEDAGFDWKILGPDVQEVDYIAWVCDQDAYACSGQKCSAQSILFMHK 341
           A    A +   +++E  G D  ++  D  ++D +A    +  ++ SGQ+C+A  ++ + +
Sbjct: 254 AISKKAGMV-PLQMELGGKDACVVLEDA-DLDLVAANIVKGGFSYSGQRCTAVKVVLIME 311

Query: 342 NWSSSGLLDKMKSLSERRKLEDLTIG---------PVLTVTTSSMIEHM 381
           + +   +++K+K+     KL  LT+G         PV+T ++++ IE +
Sbjct: 312 SVADI-VVEKVKA-----KLAKLTVGPPEADSDITPVVTESSANFIEGL 354



>AK062559 
          Length = 499

 Score = 68.2 bits (165), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 80/349 (22%), Positives = 151/349 (43%), Gaps = 34/349 (9%)

Query: 51  FVQGSW--TTSGNWNWLVDPLNGEKFIKVAEVQEAEIKPFVESLSNCPKHGLHNPLKAPE 108
           +  G W  + SG    +V+P       +V    + E+   +E+     K     PL    
Sbjct: 22  YADGEWRVSASGKSVAIVNPTTRLTQYRVQACTQEEVNKVMETAKVAQKAWARTPLWKRA 81

Query: 109 RYLMYGDISAKAANMLGQPVVSDFFAKLIQRVSPKSYQQALAEVQVSQKFLENFCGDQVR 168
             L       KAA +L +       A+ + +   K  + A++EV  S   +     + VR
Sbjct: 82  ELLH------KAAAILKEHKTP--IAECLVKEIAKPAKDAISEVVRSGDLVSYTAEEGVR 133

Query: 169 FLAR-----SFAVPGNHLGQSSNGYRWPYGPVAIITPFNFPLEIPLLQLMGALYMGNKPV 223
            L       S + PGN   +     + P G V  I PFN+P+ + + ++  AL  GN  V
Sbjct: 134 ILGEGKLLVSDSFPGNERNKYCLSSKVPLGVVLAIPPFNYPVNLAVSKIGPALIAGNALV 193

Query: 224 LKVDSKVSIVMDQMLRLLHACGMPAEDVDFINSDGITMNKLL-LEANPKMTLFTGS-SRI 281
           LK  ++ ++    M+   H  G P   ++ +   G  +   L +        FTG  + I
Sbjct: 194 LKPPTQGAVAALHMVHCFHLAGFPKGLINCVTGKGSEIGDFLTMHPGVNCISFTGGDTGI 253

Query: 282 AEKLAADLKGKIKLEDAGFDWKILGPDVQEVDYIAWVCDQDAYACSGQKCSAQSILFMHK 341
           A    A +   +++E  G D  ++  D  ++D +A    +  ++ SGQ+C+A  ++ + +
Sbjct: 254 AISKKAGMV-PLQMELGGKDACVVLEDA-DLDLVAANIVKGGFSYSGQRCTAVKVVLIME 311

Query: 342 NWSSSGLLDKMKSLSERRKLEDLTIG---------PVLTVTTSSMIEHM 381
           + +   +++K+K+     KL  LT+G         PV+T ++++ IE +
Sbjct: 312 SVADI-VVEKVKA-----KLAKLTVGPPEADSDITPVVTESSANFIEGL 354



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.135    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,160,195
Number of extensions: 624029
Number of successful extensions: 1511
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 1520
Number of HSP's successfully gapped: 4
Length of query: 464
Length of database: 17,035,801
Length adjustment: 104
Effective length of query: 360
Effective length of database: 11,605,545
Effective search space: 4177996200
Effective search space used: 4177996200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 158 (65.5 bits)