BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0515700 Os05g0515700|AK069076
         (641 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0515700  Colicin E3, catalytic domain containing protein    1188   0.0  
Os01g0753000  Colicin E3, catalytic domain containing protein     328   1e-89

>Os05g0515700 Colicin E3, catalytic domain containing protein
          Length = 641

 Score = 1188 bits (3074), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 576/614 (93%), Positives = 576/614 (93%)

Query: 28  HQQFRNLAKTRVDDLQEMFSGLQSARKESRSADAALLEEQVHHMLREWRAELNVPSPAXX 87
           HQQFRNLAKTRVDDLQEMFSGLQSARKESRSADAALLEEQVHHMLREWRAELNVPSPA  
Sbjct: 28  HQQFRNLAKTRVDDLQEMFSGLQSARKESRSADAALLEEQVHHMLREWRAELNVPSPASS 87

Query: 88  XXXXXXXXXXREASDPPSETLRLLQLAGAEEEDDATSKLVMPRSPMPMQSSHEGHNLSPV 147
                     REASDPPSETLRLLQLAGAEEEDDATSKLVMPRSPMPMQSSHEGHNLSPV
Sbjct: 88  LQNSQSQGNNREASDPPSETLRLLQLAGAEEEDDATSKLVMPRSPMPMQSSHEGHNLSPV 147

Query: 148 LQGGTMAGGAAELMVPRSPLQQMPXXXXXXXXXXXXXXNLQGEAVMGSTAATAAPHLGQG 207
           LQGGTMAGGAAELMVPRSPLQQMP              NLQGEAVMGSTAATAAPHLGQG
Sbjct: 148 LQGGTMAGGAAELMVPRSPLQQMPSSHQSHGHGQDGGQNLQGEAVMGSTAATAAPHLGQG 207

Query: 208 MQGDCGGMAGVTNAMFHDQLYYIDHELNIDDFLQDDDYKINLPGSNPDGPNTMQGIGQLE 267
           MQGDCGGMAGVTNAMFHDQLYYIDHELNIDDFLQDDDYKINLPGSNPDGPNTMQGIGQLE
Sbjct: 208 MQGDCGGMAGVTNAMFHDQLYYIDHELNIDDFLQDDDYKINLPGSNPDGPNTMQGIGQLE 267

Query: 268 HQQYNLPLDLPPNSFVDANNSAQSSGDVFFHMSDLLTTMCPSPSQYLGPKCALWDCGRPV 327
           HQQYNLPLDLPPNSFVDANNSAQSSGDVFFHMSDLLTTMCPSPSQYLGPKCALWDCGRPV
Sbjct: 268 HQQYNLPLDLPPNSFVDANNSAQSSGDVFFHMSDLLTTMCPSPSQYLGPKCALWDCGRPV 327

Query: 328 RGSDECQHYCNPYHXXXXXXXXXXXXTRPVMRPRGIDLKDGPLFAALSAKVQGKNVGIPV 387
           RGSDECQHYCNPYH            TRPVMRPRGIDLKDGPLFAALSAKVQGKNVGIPV
Sbjct: 328 RGSDECQHYCNPYHAGLALNDDGLLGTRPVMRPRGIDLKDGPLFAALSAKVQGKNVGIPV 387

Query: 388 CEGAATTKSPWNAPELFDLSLLEGESLREWLFFDTPRRAFDSGNRKQRSLPDYNGRGWHE 447
           CEGAATTKSPWNAPELFDLSLLEGESLREWLFFDTPRRAFDSGNRKQRSLPDYNGRGWHE
Sbjct: 388 CEGAATTKSPWNAPELFDLSLLEGESLREWLFFDTPRRAFDSGNRKQRSLPDYNGRGWHE 447

Query: 448 SRKQVMKDFGGLKRSYYMDPQPSSNYEWHLFEYETNDSDALALYRLEYKSSDTKRSVKSK 507
           SRKQVMKDFGGLKRSYYMDPQPSSNYEWHLFEYETNDSDALALYRLEYKSSDTKRSVKSK
Sbjct: 448 SRKQVMKDFGGLKRSYYMDPQPSSNYEWHLFEYETNDSDALALYRLEYKSSDTKRSVKSK 507

Query: 508 LASSPLSEIQQQMVRLSADSPVESKRTARSRAKANQKDNNSNAYPALNTPVQVSASNAHQ 567
           LASSPLSEIQQQMVRLSADSPVESKRTARSRAKANQKDNNSNAYPALNTPVQVSASNAHQ
Sbjct: 508 LASSPLSEIQQQMVRLSADSPVESKRTARSRAKANQKDNNSNAYPALNTPVQVSASNAHQ 567

Query: 568 TMSVNTPDQVNVSNAYQTMPLNTPNQPGPSNAYHAASQMDQMTFLDGSVVYGPHLPYGYS 627
           TMSVNTPDQVNVSNAYQTMPLNTPNQPGPSNAYHAASQMDQMTFLDGSVVYGPHLPYGYS
Sbjct: 568 TMSVNTPDQVNVSNAYQTMPLNTPNQPGPSNAYHAASQMDQMTFLDGSVVYGPHLPYGYS 627

Query: 628 TERSDFYWNPSDGT 641
           TERSDFYWNPSDGT
Sbjct: 628 TERSDFYWNPSDGT 641



>Os01g0753000 Colicin E3, catalytic domain containing protein
          Length = 216

 Score =  328 bits (840), Expect = 1e-89,   Method: Compositional matrix adjust.
 Identities = 162/239 (67%), Positives = 181/239 (75%), Gaps = 34/239 (14%)

Query: 402 ELFDLSLLEGESLREWLFFDTPRRAFDSGNRKQRSLPDYNGRGWHESRKQVMKDFGGLKR 461
           ELFDL + EGES+REWLFFD PRRAF+SGNRKQRSLPDYNGRGWHESRKQVMKDFGGLKR
Sbjct: 11  ELFDLYIFEGESIREWLFFDKPRRAFESGNRKQRSLPDYNGRGWHESRKQVMKDFGGLKR 70

Query: 462 SYYMDPQPSSNYEWHLFEYETNDSDALALYRLEYKSSDTKRSVKSKLASSPLSEIQQQMV 521
           SYYMDPQPS++YEWHL+EYE ND DA ALYRLE+KSSD K++ KSKLA +PL+EIQQQMV
Sbjct: 71  SYYMDPQPSNSYEWHLYEYEINDCDAFALYRLEFKSSDAKKTAKSKLACNPLNEIQQQMV 130

Query: 522 RLSADSPVESKRTARSRAKANQKDNNSNAYPALNTPVQVSASNAHQTMSVNTPDQVNVSN 581
           RLSADSPVE+KR+ARSR KAN  D NSN Y   NT VQ S  NA+Q              
Sbjct: 131 RLSADSPVENKRSARSRTKANPNDINSNIYLVQNTTVQGSIPNAYQ-------------- 176

Query: 582 AYQTMPLNTPNQPGPSNAYHAASQMDQMTFLDGSVVYGPHLPYGYSTERSDFYWNPSDG 640
                               A SQ DQMT+L+G+VVYGPHLPYGYSTERSDFYW+ +DG
Sbjct: 177 --------------------AVSQPDQMTYLNGNVVYGPHLPYGYSTERSDFYWSSNDG 215



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.313    0.129    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 21,506,926
Number of extensions: 933264
Number of successful extensions: 1891
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1889
Number of HSP's successfully gapped: 2
Length of query: 641
Length of database: 17,035,801
Length adjustment: 107
Effective length of query: 534
Effective length of database: 11,448,903
Effective search space: 6113714202
Effective search space used: 6113714202
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 159 (65.9 bits)