BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0465400 Os05g0465400|AK070343
         (193 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0465400  Protein of unknown function DUF1218 family pro...   305   2e-83
Os05g0121000  Protein of unknown function DUF1218 family pro...   141   4e-34

>Os05g0465400 Protein of unknown function DUF1218 family protein
          Length = 193

 Score =  305 bits (780), Expect = 2e-83,   Method: Compositional matrix adjust.
 Identities = 152/174 (87%), Positives = 152/174 (87%)

Query: 1   MAVSQSAQLGMATAFFGALSFLLAILGELNKPPHGTPIRGRGVVVCKFPADPTXXXXXXX 60
           MAVSQSAQLGMATAFFGALSFLLAILGELNKPPHGTPIRGRGVVVCKFPADPT       
Sbjct: 1   MAVSQSAQLGMATAFFGALSFLLAILGELNKPPHGTPIRGRGVVVCKFPADPTVALGALS 60

Query: 61  XXXXXXXXXXXXXXXFFPYNGKPVPRKALFDYTLLYVFFHLAIGITVAGIATTAWVTASE 120
                          FFPYNGKPVPRKALFDYTLLYVFFHLAIGITVAGIATTAWVTASE
Sbjct: 61  AVAAACGAGVGALAVFFPYNGKPVPRKALFDYTLLYVFFHLAIGITVAGIATTAWVTASE 120

Query: 121 AMHRVRNVHGVDPEYACPTAKTGVLGGVAFLNLDASLFWLLCLMLAGNVKEEYF 174
           AMHRVRNVHGVDPEYACPTAKTGVLGGVAFLNLDASLFWLLCLMLAGNVKEEYF
Sbjct: 121 AMHRVRNVHGVDPEYACPTAKTGVLGGVAFLNLDASLFWLLCLMLAGNVKEEYF 174



>Os05g0121000 Protein of unknown function DUF1218 family protein
          Length = 194

 Score =  141 bits (355), Expect = 4e-34,   Method: Compositional matrix adjust.
 Identities = 76/174 (43%), Positives = 103/174 (59%), Gaps = 1/174 (0%)

Query: 1   MAVSQSAQLGMATAFFGALSFLLAILGELNKPPHGTPIRGRGVVVCKFPADPTXXXXXXX 60
           MA++ + ++ +  +FFG LSFLL ++ E  KPP+GTPI+G+ VV+CKFP+DPT       
Sbjct: 1   MALTSAQRIALTVSFFGLLSFLLGVIAENKKPPYGTPIKGKDVVICKFPSDPTIAMGSLS 60

Query: 61  XXXXXXXXXXXXXXXFFPYNGKPVPRKALFDYTLLYVFFHLAIGITVAGIATTAWVTASE 120
                          F+PY  K VPR ALF    L  FF +A  ++    A   W T +E
Sbjct: 61  VVSLVLTTIVGHAAIFYPYKSKSVPRGALFRSASLSAFFVIAELVSALAFAMLLWATVTE 120

Query: 121 AMHRVRNVHGVDPEYACPTAKTGVLGGVAFLNLDASLFWLLCLMLAGNVKEEYF 174
            +HR  N+H  D +  CPTAKTG+ GG AFL LDA+LFWL+C MLA N + +Y 
Sbjct: 121 GLHRSNNIHH-DLDTQCPTAKTGLFGGAAFLALDAALFWLVCQMLALNARSDYL 173



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.326    0.141    0.445 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,347,992
Number of extensions: 172457
Number of successful extensions: 415
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 412
Number of HSP's successfully gapped: 2
Length of query: 193
Length of database: 17,035,801
Length adjustment: 95
Effective length of query: 98
Effective length of database: 12,075,471
Effective search space: 1183396158
Effective search space used: 1183396158
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 153 (63.5 bits)