BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0456900 Os05g0456900|AK073249
(90 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os05g0456900 Conserved hypothetical protein 185 5e-48
Os01g0847100 Conserved hypothetical protein 74 3e-14
>Os05g0456900 Conserved hypothetical protein
Length = 90
Score = 185 bits (470), Expect = 5e-48, Method: Compositional matrix adjust.
Identities = 90/90 (100%), Positives = 90/90 (100%)
Query: 1 MEGFIPFIYKAIVQYRKEGQVSVGDLFFDEPSPSSYFRLPGDSGRYQQQQQAAMSVLFSQ 60
MEGFIPFIYKAIVQYRKEGQVSVGDLFFDEPSPSSYFRLPGDSGRYQQQQQAAMSVLFSQ
Sbjct: 1 MEGFIPFIYKAIVQYRKEGQVSVGDLFFDEPSPSSYFRLPGDSGRYQQQQQAAMSVLFSQ 60
Query: 61 ATTADSDAVAPRRSPLRCTVHHRRPATATP 90
ATTADSDAVAPRRSPLRCTVHHRRPATATP
Sbjct: 61 ATTADSDAVAPRRSPLRCTVHHRRPATATP 90
>Os01g0847100 Conserved hypothetical protein
Length = 86
Score = 73.6 bits (179), Expect = 3e-14, Method: Compositional matrix adjust.
Identities = 36/85 (42%), Positives = 50/85 (58%), Gaps = 1/85 (1%)
Query: 1 MEGFIPFIYKAIVQYRKEGQVSVGDLFFDEPSPSSYFRLPGDS-GRYQQQQQAAMSVLFS 59
MEG IPFI+KA+ QY++ G VS+ D+ D PSP+SY LPGDS GR+ + + + S
Sbjct: 1 MEGIIPFIFKAVAQYKEGGHVSLSDMISDRPSPASYVLLPGDSDGRHADDKTQPLRLQAS 60
Query: 60 QATTADSDAVAPRRSPLRCTVHHRR 84
+ + R S LRC+ RR
Sbjct: 61 TGSEEEVTTCTARASHLRCSTLRRR 85
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.320 0.133 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,261,788
Number of extensions: 120063
Number of successful extensions: 345
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 345
Number of HSP's successfully gapped: 2
Length of query: 90
Length of database: 17,035,801
Length adjustment: 60
Effective length of query: 30
Effective length of database: 13,902,961
Effective search space: 417088830
Effective search space used: 417088830
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 149 (62.0 bits)