BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0447000 Os05g0447000|AK108280
         (172 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0447000  Similar to Pleckstrin homology domain-containi...   303   3e-83
Os01g0856800  Pleckstrin homology-type domain containing pro...   196   9e-51

>Os05g0447000 Similar to Pleckstrin homology domain-containing protein 1 (AtPH1)
          Length = 172

 Score =  303 bits (777), Expect = 3e-83,   Method: Compositional matrix adjust.
 Identities = 151/172 (87%), Positives = 151/172 (87%)

Query: 1   MAVSLWRAVMXXXXXXXXXXXXXXXXXXXXXVEFWHGGERTGWLNKQGEYIKTWRRRWFV 60
           MAVSLWRAVM                     VEFWHGGERTGWLNKQGEYIKTWRRRWFV
Sbjct: 1   MAVSLWRAVMGAAGGGGSSSSSAAGGDAGGGVEFWHGGERTGWLNKQGEYIKTWRRRWFV 60

Query: 61  LKQGRLFWFKDAAVTRGSVPRGVIPVATCLTVKGAEDVINRQFAFELSTPTDTMYFIADS 120
           LKQGRLFWFKDAAVTRGSVPRGVIPVATCLTVKGAEDVINRQFAFELSTPTDTMYFIADS
Sbjct: 61  LKQGRLFWFKDAAVTRGSVPRGVIPVATCLTVKGAEDVINRQFAFELSTPTDTMYFIADS 120

Query: 121 EKEKEEWINSIGRSIVQHSRSVTDAEVVDYDSRPNSKPPPQPKTSEESEPSA 172
           EKEKEEWINSIGRSIVQHSRSVTDAEVVDYDSRPNSKPPPQPKTSEESEPSA
Sbjct: 121 EKEKEEWINSIGRSIVQHSRSVTDAEVVDYDSRPNSKPPPQPKTSEESEPSA 172



>Os01g0856800 Pleckstrin homology-type domain containing protein
          Length = 142

 Score =  196 bits (497), Expect = 9e-51,   Method: Compositional matrix adjust.
 Identities = 105/155 (67%), Positives = 108/155 (69%), Gaps = 24/155 (15%)

Query: 1   MAVSLWRAVMXXXXXXXXXXXXXXXXXXXXXVEFWHGGERTGWLNKQGEYIKTWRRRWFV 60
           MA SLWRAVM                     VEFW   ER GWL KQGEYIKTWRRRWFV
Sbjct: 1   MAASLWRAVMGAGASSADTDTTGG-------VEFWRSPERAGWLTKQGEYIKTWRRRWFV 53

Query: 61  LKQGRLFWFKDAAVTRGSVPRGVIPVATCLTVKGAEDVINRQFAFELSTPTDTMYFIADS 120
           LKQGRLFWFKD+ VT                VKGAED +NRQFAFELSTP +TMYFIADS
Sbjct: 54  LKQGRLFWFKDSGVT----------------VKGAEDTLNRQFAFELSTPAETMYFIADS 97

Query: 121 EKEKEEWINSIGRSIVQHSRSVTDAEVVDYDS-RP 154
           EKEKEEWINSIGRSIVQHSRSVTDAEVVDYDS RP
Sbjct: 98  EKEKEEWINSIGRSIVQHSRSVTDAEVVDYDSGRP 132



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.131    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,124,414
Number of extensions: 247871
Number of successful extensions: 1681
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1678
Number of HSP's successfully gapped: 2
Length of query: 172
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 78
Effective length of database: 12,127,685
Effective search space: 945959430
Effective search space used: 945959430
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 152 (63.2 bits)