BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0446900 Os05g0446900|AK101748
         (373 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0446900  Glycoside hydrolase, starch-binding domain con...   656   0.0  
Os01g0856900  Glycoside hydrolase, starch-binding domain con...   174   8e-44

>Os05g0446900 Glycoside hydrolase, starch-binding domain containing protein
          Length = 373

 Score =  656 bits (1692), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 331/373 (88%), Positives = 331/373 (88%)

Query: 1   MEAAALGVPLVQXXXXXXXXXXXXXXXXXXXXXXXXFHAPPGRRRTALVAALPEPLQPLS 60
           MEAAALGVPLVQ                        FHAPPGRRRTALVAALPEPLQPLS
Sbjct: 1   MEAAALGVPLVQVRAAVAAYGRGAGKGKGKRRVVGAFHAPPGRRRTALVAALPEPLQPLS 60

Query: 61  PAQDGAVALASTEADGGEEVHGDVASAEISSPSGVLGKTVRVRFVLKRECTFGQSFHLVG 120
           PAQDGAVALASTEADGGEEVHGDVASAEISSPSGVLGKTVRVRFVLKRECTFGQSFHLVG
Sbjct: 61  PAQDGAVALASTEADGGEEVHGDVASAEISSPSGVLGKTVRVRFVLKRECTFGQSFHLVG 120

Query: 121 DDPALGLWDPSKAVPLDWSEGHDWTVEKDLPANKLIEYKFVLQDLSGKLHWQNGRNRSVQ 180
           DDPALGLWDPSKAVPLDWSEGHDWTVEKDLPANKLIEYKFVLQDLSGKLHWQNGRNRSVQ
Sbjct: 121 DDPALGLWDPSKAVPLDWSEGHDWTVEKDLPANKLIEYKFVLQDLSGKLHWQNGRNRSVQ 180

Query: 181 TGETANILVVYEDWGNANSQTVEEEGKVSIGMEEGKLSVGMEEAVVPDDSESRDDIIVAD 240
           TGETANILVVYEDWGNANSQTVEEEGKVSIGMEEGKLSVGMEEAVVPDDSESRDDIIVAD
Sbjct: 181 TGETANILVVYEDWGNANSQTVEEEGKVSIGMEEGKLSVGMEEAVVPDDSESRDDIIVAD 240

Query: 241 ELQVDDNLAVMQNESSVREDDKKSTVGTVTSVQAELMKLHEANQPELIVDEPQIQEALPE 300
           ELQVDDNLAVMQNESSVREDDKKSTVGTVTSVQAELMKLHEANQPELIVDEPQIQEALPE
Sbjct: 241 ELQVDDNLAVMQNESSVREDDKKSTVGTVTSVQAELMKLHEANQPELIVDEPQIQEALPE 300

Query: 301 TADTEPENGGVATCADDRYAESXXXXXXXXXXXXXXXXXXENRWTGAFEHELLWGWKALQ 360
           TADTEPENGGVATCADDRYAES                  ENRWTGAFEHELLWGWKALQ
Sbjct: 301 TADTEPENGGVATCADDRYAESTDDDGVPVGGTDDDGVPVENRWTGAFEHELLWGWKALQ 360

Query: 361 QLLMSLGFKMDTS 373
           QLLMSLGFKMDTS
Sbjct: 361 QLLMSLGFKMDTS 373



>Os01g0856900 Glycoside hydrolase, starch-binding domain containing protein
          Length = 379

 Score =  174 bits (442), Expect = 8e-44,   Method: Compositional matrix adjust.
 Identities = 131/355 (36%), Positives = 179/355 (50%), Gaps = 47/355 (13%)

Query: 43  RRRTALVAALPEPL---QPLSPAQDGAVALASTEA---------------DGGEEVHGDV 84
           RRR  LVA+L EPL   QPLS +     A+  T A               D   +V  D 
Sbjct: 44  RRRPLLVASLGEPLITAQPLSSSLGDGAAVHETLARSDSVIPSLKPSHCVDHSVQVDADE 103

Query: 85  ASAEISS---PSGVLGKTVRVRFVLKRECTFGQSFHLVGDDPALGLWDPSKAVPLDWSEG 141
            +    +   P  V  KTV V+FVL++ C FGQ F +VGD  ALGLW+P+KA  LDWSE 
Sbjct: 104 ETGSTKTLPPPDDVPTKTVHVKFVLQKRCAFGQRFLVVGDVAALGLWNPAKAAALDWSED 163

Query: 142 HDWTVEKDLPANKLIEYKFVLQDLSGKLHWQNGRNRSVQTGETANILVVYEDWGNANSQT 201
           H WTV+K+LPA + IE+KF+LQD SG + WQ+GRNR +   +T+N L+V EDW  A +Q 
Sbjct: 164 HVWTVKKELPAERSIEFKFLLQDRSGHVEWQHGRNRILHVADTSNTLIVCEDWDEAKNQQ 223

Query: 202 VEEEGKVSIGMEEGKLSVGMEEAVVPDDSESRDDIIVADELQVDDNLAVMQNESSVREDD 261
           V EE    IG  +G  S               D +   DELQ+ +     +   +V  DD
Sbjct: 224 VSEE----IGDADGIFS-------------GSDGVFQEDELQLGEEQETNKG-VTVGVDD 265

Query: 262 KKSTVGTVTSVQAELMKLHEANQPELIVDE-PQIQEALPETADTEPENGGVATCADDRYA 320
            KS +  VT +  E+M  ++A QP+L +D+  +I + L   A+   ++G     A    A
Sbjct: 266 AKSAL--VTYIYREMMGANDAIQPQLALDKHHKIPDELSGEANMAAQDGNHTATA--AAA 321

Query: 321 ESXXXXXXXXXXXXXXXXXXENRWTG---AFEHELLWGWKALQQLLMSLGFKMDT 372
                               EN   G    F +++ W  KALQQLL SLGF++ T
Sbjct: 322 SGFAGSNGEDAILHKEGDPVENNRLGLASIFFNDMAWTRKALQQLLRSLGFQIGT 376



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.311    0.130    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 12,244,309
Number of extensions: 529929
Number of successful extensions: 1525
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1518
Number of HSP's successfully gapped: 2
Length of query: 373
Length of database: 17,035,801
Length adjustment: 102
Effective length of query: 271
Effective length of database: 11,709,973
Effective search space: 3173402683
Effective search space used: 3173402683
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 157 (65.1 bits)