BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0361200 Os05g0361200|AK073873
         (526 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0361200  Similar to Ferrochelatase                          1016   0.0  
Os09g0297000  Similar to Ferrochelatase II, chloroplast prec...   540   e-153

>Os05g0361200 Similar to Ferrochelatase
          Length = 526

 Score = 1016 bits (2626), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 499/526 (94%), Positives = 499/526 (94%)

Query: 1   MWSSSQASTRGVIEVGRVEAGPSHFPKRPAPRNSSRVNLSRTYAIKSCSVSSRTGLCLGQ 60
           MWSSSQASTRGVIEVGRVEAGPSHFPKRPAPRNSSRVNLSRTYAIKSCSVSSRTGLCLGQ
Sbjct: 1   MWSSSQASTRGVIEVGRVEAGPSHFPKRPAPRNSSRVNLSRTYAIKSCSVSSRTGLCLGQ 60

Query: 61  CYHKKSSACKCKLGWSSQPXXXXXXXXXXXXXXXEAVLTSQSDFTKLLVGNEKIGVLLLN 120
           CYHKKSSACKCKLGWSSQP               EAVLTSQSDFTKLLVGNEKIGVLLLN
Sbjct: 61  CYHKKSSACKCKLGWSSQPLSSLRHHLRVHSSASEAVLTSQSDFTKLLVGNEKIGVLLLN 120

Query: 121 LGGPETLDDVQPFLFNLFADPDIIRLPRLFRFLQKPLAQFISVVRAPKSKEGYASIGGGS 180
           LGGPETLDDVQPFLFNLFADPDIIRLPRLFRFLQKPLAQFISVVRAPKSKEGYASIGGGS
Sbjct: 121 LGGPETLDDVQPFLFNLFADPDIIRLPRLFRFLQKPLAQFISVVRAPKSKEGYASIGGGS 180

Query: 181 PLRQITDAQAEALRKALCDKDIPAKVYVGMRYWHPFTEEAIEQIKRDGITKLVVLPLYPQ 240
           PLRQITDAQAEALRKALCDKDIPAKVYVGMRYWHPFTEEAIEQIKRDGITKLVVLPLYPQ
Sbjct: 181 PLRQITDAQAEALRKALCDKDIPAKVYVGMRYWHPFTEEAIEQIKRDGITKLVVLPLYPQ 240

Query: 241 FSISTSGSSLRLLEGIFREDEYLVNMQHTVIPSWYQREGYIKAMATLIEKELRTFSEPQK 300
           FSISTSGSSLRLLEGIFREDEYLVNMQHTVIPSWYQREGYIKAMATLIEKELRTFSEPQK
Sbjct: 241 FSISTSGSSLRLLEGIFREDEYLVNMQHTVIPSWYQREGYIKAMATLIEKELRTFSEPQK 300

Query: 301 VMIFFSAHGVPLAYVEEAGDPYKAEMEECVDLIMEELEKRGITNSCTLAYQSRVGPVEWL 360
           VMIFFSAHGVPLAYVEEAGDPYKAEMEECVDLIMEELEKRGITNSCTLAYQSRVGPVEWL
Sbjct: 301 VMIFFSAHGVPLAYVEEAGDPYKAEMEECVDLIMEELEKRGITNSCTLAYQSRVGPVEWL 360

Query: 361 RPYTDETIIELGQKGVKSLLAVPISFVSEHIETLEEIDVEYKELALESGIKHWGRVPALG 420
           RPYTDETIIELGQKGVKSLLAVPISFVSEHIETLEEIDVEYKELALESGIKHWGRVPALG
Sbjct: 361 RPYTDETIIELGQKGVKSLLAVPISFVSEHIETLEEIDVEYKELALESGIKHWGRVPALG 420

Query: 421 CEPTFITDLADAVIESLPYVGAMAVSNLEARQPLVPLGSVEELLAAYDSKRDELPPPVTV 480
           CEPTFITDLADAVIESLPYVGAMAVSNLEARQPLVPLGSVEELLAAYDSKRDELPPPVTV
Sbjct: 421 CEPTFITDLADAVIESLPYVGAMAVSNLEARQPLVPLGSVEELLAAYDSKRDELPPPVTV 480

Query: 481 WEWGWTKSAETWNGRXXXXXXXXXXXXEVTTGEGFLHQWGILPLFH 526
           WEWGWTKSAETWNGR            EVTTGEGFLHQWGILPLFH
Sbjct: 481 WEWGWTKSAETWNGRAAMLAVLALLVLEVTTGEGFLHQWGILPLFH 526



>Os09g0297000 Similar to Ferrochelatase II, chloroplast precursor (EC 4.99.1.1)
           (Protoheme ferro-lyase) (Heme synthetase)
          Length = 482

 Score =  540 bits (1391), Expect = e-153,   Method: Compositional matrix adjust.
 Identities = 250/338 (73%), Positives = 296/338 (87%)

Query: 112 EKIGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPRLFRFLQKPLAQFISVVRAPKSKE 171
           EK+GVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPRLFRFLQ+PLA+ IS  RAPKSKE
Sbjct: 105 EKVGVLLLNLGGPETLDDVQPFLFNLFADPDIIRLPRLFRFLQRPLAKLISTFRAPKSKE 164

Query: 172 GYASIGGGSPLRQITDAQAEALRKALCDKDIPAKVYVGMRYWHPFTEEAIEQIKRDGITK 231
           GYASIGGGSPLR+ITD QA AL+ AL  K++ A +YVGMRYW+PFTEEAI+QIK+D ITK
Sbjct: 165 GYASIGGGSPLRKITDEQANALKVALKKKNLNANIYVGMRYWYPFTEEAIDQIKKDKITK 224

Query: 232 LVVLPLYPQFSISTSGSSLRLLEGIFREDEYLVNMQHTVIPSWYQREGYIKAMATLIEKE 291
           LVVLPLYPQ+SISTSGSS+R+L+ I +ED Y   +  ++I SWYQR+GY+K+MA LIEKE
Sbjct: 225 LVVLPLYPQYSISTSGSSIRVLQNIVKEDSYFAGLPISIIESWYQRDGYVKSMADLIEKE 284

Query: 292 LRTFSEPQKVMIFFSAHGVPLAYVEEAGDPYKAEMEECVDLIMEELEKRGITNSCTLAYQ 351
           L  FS P++VMIFFSAHGVPL YV +AGDPY+ +ME+C+ LIM EL+ RGI NS TLAYQ
Sbjct: 285 LSIFSNPEEVMIFFSAHGVPLTYVTDAGDPYRDQMEDCIALIMGELKSRGILNSHTLAYQ 344

Query: 352 SRVGPVEWLRPYTDETIIELGQKGVKSLLAVPISFVSEHIETLEEIDVEYKELALESGIK 411
           SRVGPV+WL+PYTDE ++ELGQ+GVKSLLAVP+SFVSEHIETLEEID+EYKELALESGI+
Sbjct: 345 SRVGPVQWLKPYTDEVLVELGQQGVKSLLAVPVSFVSEHIETLEEIDMEYKELALESGIE 404

Query: 412 HWGRVPALGCEPTFITDLADAVIESLPYVGAMAVSNLE 449
           +WGRVPALGC  +FI+DLADAV+E+LP   A+    ++
Sbjct: 405 NWGRVPALGCTSSFISDLADAVVEALPSASALVTKKVD 442



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.135    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 17,165,067
Number of extensions: 678422
Number of successful extensions: 1764
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1764
Number of HSP's successfully gapped: 2
Length of query: 526
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 421
Effective length of database: 11,553,331
Effective search space: 4863952351
Effective search space used: 4863952351
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 158 (65.5 bits)