BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0335100 Os05g0335100|AK107763
         (223 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0335100  Saposin B domain containing protein                 454   e-128
Os01g0166700  Saposin family protein                              194   3e-50
Os12g0112250  Saposin B domain containing protein                 140   8e-34

>Os05g0335100 Saposin B domain containing protein
          Length = 223

 Score =  454 bits (1169), Expect = e-128,   Method: Compositional matrix adjust.
 Identities = 223/223 (100%), Positives = 223/223 (100%)

Query: 1   MGPRVVFMFIIAVMLLGDTSLAFDNAVGEKISNMETSLTMKVDPQLCQICEEFATEALFY 60
           MGPRVVFMFIIAVMLLGDTSLAFDNAVGEKISNMETSLTMKVDPQLCQICEEFATEALFY
Sbjct: 1   MGPRVVFMFIIAVMLLGDTSLAFDNAVGEKISNMETSLTMKVDPQLCQICEEFATEALFY 60

Query: 61  LNENETQVEIIATLHQACSKFPSFKLECTKLVDYYVSLFFTKVTSLSPEEFCESVSLCHK 120
           LNENETQVEIIATLHQACSKFPSFKLECTKLVDYYVSLFFTKVTSLSPEEFCESVSLCHK
Sbjct: 61  LNENETQVEIIATLHQACSKFPSFKLECTKLVDYYVSLFFTKVTSLSPEEFCESVSLCHK 120

Query: 121 VTFIRLPRHEDSCDLCHEVVDEILTDLENPDVELKIIEVLLKGCNNAENFVQKCKKLIIQ 180
           VTFIRLPRHEDSCDLCHEVVDEILTDLENPDVELKIIEVLLKGCNNAENFVQKCKKLIIQ
Sbjct: 121 VTFIRLPRHEDSCDLCHEVVDEILTDLENPDVELKIIEVLLKGCNNAENFVQKCKKLIIQ 180

Query: 181 NAPIILEHIKKFLKKRDFCNSIHVCGGKIIPARAGDLGALSAA 223
           NAPIILEHIKKFLKKRDFCNSIHVCGGKIIPARAGDLGALSAA
Sbjct: 181 NAPIILEHIKKFLKKRDFCNSIHVCGGKIIPARAGDLGALSAA 223



>Os01g0166700 Saposin family protein
          Length = 226

 Score =  194 bits (494), Expect = 3e-50,   Method: Compositional matrix adjust.
 Identities = 96/216 (44%), Positives = 142/216 (65%), Gaps = 16/216 (7%)

Query: 21  LAFDNAVGEK-------------ISNMETSLTMKVDPQLCQICEEFATEALFYLNENETQ 67
           LAF  AV E              + N +T LT   + +LCQ+CE+++TEALFYL +NETQ
Sbjct: 13  LAFSVAVAESRDSFNVLAHKSFPLENKKTGLT-SANGKLCQLCEQYSTEALFYLQQNETQ 71

Query: 68  VEIIATLHQACSKFPSFKLECTKLVDYYVSLFFTKVTSLSPEEFCESVSLCHKVTFIRLP 127
            EI++ LH AC+     K +C  LVDYY+ LFF +V+ ++PE+FCESV LC K T +RLP
Sbjct: 72  TEILSILHHACANVGPLKQQCITLVDYYIPLFFLEVSMVTPEKFCESVHLCRKGTMLRLP 131

Query: 128 RHEDSCDLCHEVVDEILTDLENPDVELKIIEVLLKGCNNAENFVQKCKKLIIQNAPIILE 187
              D+C +CH V+ E+L  L++PD++L+I+E+ LK C+ A+N+VQ+CKK++++  P+IL 
Sbjct: 132 SRGDTCGICHHVLVEVLIMLKDPDMQLEIVEIFLKACSKADNYVQQCKKMVLEYTPLILV 191

Query: 188 HIKKFLKKRDFCNSIHVCGGKIIPARAGDLGALSAA 223
             +KFL+  D C++IH C  K     + +   LSAA
Sbjct: 192 KSQKFLETTDVCSTIHAC--KTGTQASAETMLLSAA 225



>Os12g0112250 Saposin B domain containing protein
          Length = 245

 Score =  140 bits (353), Expect = 8e-34,   Method: Compositional matrix adjust.
 Identities = 63/164 (38%), Positives = 103/164 (62%), Gaps = 1/164 (0%)

Query: 46  LCQICEEFATEALFYLNENETQVEIIATLHQACSKFPSFKLECTKLVDYYVSLFFTKVTS 105
           LC  CE   +EA+ +L+E + Q +I+  LH  CS+  SF+ +C + +D Y +L F K+  
Sbjct: 66  LCSACENITSEAVNFLSEKQIQDKIMTILHDTCSQTFSFEQKCLETMDSYATLVFAKIAE 125

Query: 106 LSPEEFCESVSLCHKVTFIRLPRHEDSCDLCHEVVDEILTDLENPDVELKIIEVLLKGCN 165
           + P+EFC+   LC  +  +   + E +C  CH ++DEI++ L++PD E +II++LLK CN
Sbjct: 126 IKPDEFCKQYGLCRDMALLSAMKSESTCVFCHHIIDEIMSKLKDPDAEFEIIQLLLKECN 185

Query: 166 NAENFVQKCKKLIIQNAPIILEHIKKFLKKRDFCNSIHVC-GGK 208
             E   Q+CK++++Q  P++L + +KFL+K D C  I  C  GK
Sbjct: 186 KIEGHQQQCKRMVLQYVPLVLVNGEKFLEKNDVCAMIQACDAGK 229



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.324    0.138    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,976,527
Number of extensions: 262049
Number of successful extensions: 847
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 841
Number of HSP's successfully gapped: 3
Length of query: 223
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 126
Effective length of database: 11,971,043
Effective search space: 1508351418
Effective search space used: 1508351418
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 154 (63.9 bits)