BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0203400 Os05g0203400|Os05g0203400
         (200 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0203400  dTDP-glucose 4,6-dehydratase family protein         291   2e-79
Os03g0278200  NAD-dependent epimerase/dehydratase family pro...   172   2e-43

>Os05g0203400 dTDP-glucose 4,6-dehydratase family protein
          Length = 200

 Score =  291 bits (745), Expect = 2e-79,   Method: Compositional matrix adjust.
 Identities = 148/181 (81%), Positives = 148/181 (81%)

Query: 20  YTTPRNILITGAAGFIASHVANRLLRDHPSYRVVALDKLDDYCATLNNLAPAMASPNFAF 79
           YTTPRNILITGAAGFIASHVANRLLRDHPSYRVVALDKLDDYCATLNNLAPAMASPNFAF
Sbjct: 20  YTTPRNILITGAAGFIASHVANRLLRDHPSYRVVALDKLDDYCATLNNLAPAMASPNFAF 79

Query: 80  VRGDVASADLVLHLLLAERINTVMHFAAHTHVDDNSFEFTKNNVLGTHVLLEPCRRKXXX 139
           VRGDVASADLVLHLLLAERINTVMHFAAHTHVDDNSFEFTKNNVLGTHVLLEPCRRK   
Sbjct: 80  VRGDVASADLVLHLLLAERINTVMHFAAHTHVDDNSFEFTKNNVLGTHVLLEPCRRKAPP 139

Query: 140 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCSPPTNLLKSGQRHLAVSFSISYTRYNGLK 199
                                         CSPPTNLLKSGQRHLAVSFSISYTRYNGLK
Sbjct: 140 APSAASSTSAPTRSTATLPRRATTRRRGRGCSPPTNLLKSGQRHLAVSFSISYTRYNGLK 199

Query: 200 L 200
           L
Sbjct: 200 L 200



>Os03g0278200 NAD-dependent epimerase/dehydratase family protein
          Length = 675

 Score =  172 bits (435), Expect = 2e-43,   Method: Composition-based stats.
 Identities = 80/115 (69%), Positives = 98/115 (85%), Gaps = 4/115 (3%)

Query: 23  PRNILITGAAGFIASHVANRLLRDHPSYRVVALDKLDDYCATLNNLAPAMASPNFAFVRG 82
           P+NILITGAAGFIASHVANRL+R++P Y++V LDKLD YC++L+NL P+  SPNF FV+G
Sbjct: 6   PKNILITGAAGFIASHVANRLVRNYPHYKIVVLDKLD-YCSSLSNLNPSRPSPNFKFVKG 64

Query: 83  DVASADLVLHLLLAERINTVMHFAAHTHVDD---NSFEFTKNNVLGTHVLLEPCR 134
           D+ASADLV +LL  E I+T+MHFAA THVD+   NSFEFTKNN+ GTHVLLE C+
Sbjct: 65  DIASADLVNYLLTTESIDTIMHFAAQTHVDNSFGNSFEFTKNNIYGTHVLLEACK 119



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.324    0.136    0.406 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,922,809
Number of extensions: 148091
Number of successful extensions: 481
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 489
Number of HSP's successfully gapped: 2
Length of query: 200
Length of database: 17,035,801
Length adjustment: 96
Effective length of query: 104
Effective length of database: 12,023,257
Effective search space: 1250418728
Effective search space used: 1250418728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 153 (63.5 bits)