BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os05g0195400 Os05g0195400|AK072351
(90 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os05g0195400 Conserved hypothetical protein 125 5e-30
Os11g0481500 Conserved hypothetical protein 63 4e-11
>Os05g0195400 Conserved hypothetical protein
Length = 90
Score = 125 bits (315), Expect = 5e-30, Method: Compositional matrix adjust.
Identities = 62/62 (100%), Positives = 62/62 (100%)
Query: 29 WRHTATASGASPPLDLAGGETVGSCGPCDEVPTGGEAVGSRSWHRAPPRQQRQAPHGGGA 88
WRHTATASGASPPLDLAGGETVGSCGPCDEVPTGGEAVGSRSWHRAPPRQQRQAPHGGGA
Sbjct: 29 WRHTATASGASPPLDLAGGETVGSCGPCDEVPTGGEAVGSRSWHRAPPRQQRQAPHGGGA 88
Query: 89 FE 90
FE
Sbjct: 89 FE 90
>Os11g0481500 Conserved hypothetical protein
Length = 102
Score = 63.2 bits (152), Expect = 4e-11, Method: Compositional matrix adjust.
Identities = 31/46 (67%), Positives = 36/46 (78%)
Query: 40 PPLDLAGGETVGSCGPCDEVPTGGEAVGSRSWHRAPPRQQRQAPHG 85
PPLDLAGGETVGS GPC E+ GGEAVGSR+ RA PRQ+++ H
Sbjct: 37 PPLDLAGGETVGSRGPCGELSMGGEAVGSRTQRRASPRQRQRDLHN 82
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.313 0.133 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,559,643
Number of extensions: 163021
Number of successful extensions: 518
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 518
Number of HSP's successfully gapped: 2
Length of query: 90
Length of database: 17,035,801
Length adjustment: 60
Effective length of query: 30
Effective length of database: 13,902,961
Effective search space: 417088830
Effective search space used: 417088830
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 149 (62.0 bits)