BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0149800 Os05g0149800|AK070081
         (488 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0149800  EF-Hand type domain containing protein              901   0.0  
Os10g0476600  Similar to Protein phosphatase 2A 62 kDa B\'\'...    88   2e-17

>Os05g0149800 EF-Hand type domain containing protein
          Length = 488

 Score =  901 bits (2328), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 434/448 (96%), Positives = 434/448 (96%)

Query: 27  RRIPPASSMPWVRNLRRFVGTGAGLGSEALMELETKRILLEIFKERQRKSMEAGAIPSFY 86
           RRIPPASSMPWVRNLRRFVGTGAGLGSEALMELETKRILLEIFKERQRKSMEAGAIPSFY
Sbjct: 27  RRIPPASSMPWVRNLRRFVGTGAGLGSEALMELETKRILLEIFKERQRKSMEAGAIPSFY 86

Query: 87  KKKPEDGSISNRVQKLAKYRFLKKQSELLLNADDLDAMWVCLRENCVIDDATGAEKMNYE 146
           KKKPEDGSISNRVQKLAKYRFLKKQSELLLNADDLDAMWVCLRENCVIDDATGAEKMNYE
Sbjct: 87  KKKPEDGSISNRVQKLAKYRFLKKQSELLLNADDLDAMWVCLRENCVIDDATGAEKMNYE 146

Query: 147 DFCHIATVCTEQIGQKCKRFFSPSNFMKFEKDDSGRIAILPFYLYVMRTVSLTQARIDMS 206
           DFCHIATVCTEQIGQKCKRFFSPSNFMKFEKDDSGRIAILPFYLYVMRTVSLTQARIDMS
Sbjct: 147 DFCHIATVCTEQIGQKCKRFFSPSNFMKFEKDDSGRIAILPFYLYVMRTVSLTQARIDMS 206

Query: 207 ELDEDSDGFLQPHEMEAYIRGLIPNLAQLRDMPSAFVQMYCRIAARKFFFFCDPHRRGKA 266
           ELDEDSDGFLQPHEMEAYIRGLIPNLAQLRDMPSAFVQMYCRIAARKFFFFCDPHRRGKA
Sbjct: 207 ELDEDSDGFLQPHEMEAYIRGLIPNLAQLRDMPSAFVQMYCRIAARKFFFFCDPHRRGKA 266

Query: 267 CIKKVLLSNCXXXXXXXXXXXXXXVTDTEQAENWFSLTSAQRICDMFLALDKDTNGTLSK 326
           CIKKVLLSNC              VTDTEQAENWFSLTSAQRICDMFLALDKDTNGTLSK
Sbjct: 267 CIKKVLLSNCLQELMELHQESEEEVTDTEQAENWFSLTSAQRICDMFLALDKDTNGTLSK 326

Query: 327 QELKEYADGTLTDIFIERVFDEHVRRSKVGGGNSREMDFESFLDFVLALENKDTPEGLTY 386
           QELKEYADGTLTDIFIERVFDEHVRRSKVGGGNSREMDFESFLDFVLALENKDTPEGLTY
Sbjct: 327 QELKEYADGTLTDIFIERVFDEHVRRSKVGGGNSREMDFESFLDFVLALENKDTPEGLTY 386

Query: 387 LFRCLDLNGRGFLTTADIHTLFRDVHQKWIEGGNYELCIEDVRDEIWDMVKPADPLRIAL 446
           LFRCLDLNGRGFLTTADIHTLFRDVHQKWIEGGNYELCIEDVRDEIWDMVKPADPLRIAL
Sbjct: 387 LFRCLDLNGRGFLTTADIHTLFRDVHQKWIEGGNYELCIEDVRDEIWDMVKPADPLRIAL 446

Query: 447 TDLLSCKQGGTIASMLIDVRGFWAHDNR 474
           TDLLSCKQGGTIASMLIDVRGFWAHDNR
Sbjct: 447 TDLLSCKQGGTIASMLIDVRGFWAHDNR 474



>Os10g0476600 Similar to Protein phosphatase 2A 62 kDa B\'\' regulatory subunit
           (Protein phosphatase 2A 62 kDa B regulatory subunit)
           (Protein phosphatase 2A alpha)
          Length = 541

 Score = 87.8 bits (216), Expect = 2e-17,   Method: Compositional matrix adjust.
 Identities = 74/318 (23%), Positives = 133/318 (41%), Gaps = 13/318 (4%)

Query: 162 KCKRFFSPSNFMKFEKDDSGRIAILPFYLYVMR--TVSLTQARIDMSELDEDSDGFLQPH 219
           K   FFS   F K +   SG +    F  Y +    +++  A      L +    +L   
Sbjct: 179 KLPSFFSSVLFKKIDAAGSGTVTRDAFVDYWINDNKITMDTASQIFEILRKPDYNYLTQE 238

Query: 220 EMEAYIRGLI---PNLAQLRDMPSAFVQMYCRIAARKFFFFCDPHRRGKACIKKVLLSNC 276
           + +  ++ L+   P L  L+  P  F + Y      + F+  +    G   ++++   N 
Sbjct: 239 DFKPVLKELLATHPGLEFLQGTPE-FQERYAETVIYRIFYSINRAGNGHLTLRELKRGNL 297

Query: 277 XXXXXXXXXXXXXXVTDTEQAENWFSLTSAQRICDMFLALDKDTNGTLSKQELKEYADGT 336
                           D  +   +FS      I   F  LD D +  + K+ L  Y + +
Sbjct: 298 IAAMQQLDEEE-----DINKVLRYFSYEHFYVIYCKFWELDTDHDFLIDKENLIRYGNHS 352

Query: 337 LTDIFIERVFDEHVRRSKVGGGNSREMDFESFLDFVLALENKDTPEGLTYLFRCLDLNGR 396
           LT   ++R+F +  R  K       +M +E F+ F+L+ E+K +   L Y F+C+DL+G 
Sbjct: 353 LTYRIVDRIFSQVPR--KFTSMTEGKMGYEDFVYFILSEEDKSSEPSLEYWFKCIDLDGN 410

Query: 397 GFLTTADIHTLFRDVHQKWIEGGNYELCIEDVRDEIWDMVKPADPLRIALTDLLSCKQGG 456
           G LTT ++   + +   +        +  ED+  ++ DM+ P +     L DL  CK  G
Sbjct: 411 GILTTTEMQFFYEEQLHRMECMAQEPVLFEDILCQMIDMIGPENECYFTLRDLKRCKLSG 470

Query: 457 TIASMLIDVRGFWAHDNR 474
            I ++L ++  F A + R
Sbjct: 471 NIFNILFNLNKFMAFETR 488



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.323    0.138    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 15,296,939
Number of extensions: 636328
Number of successful extensions: 1312
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1309
Number of HSP's successfully gapped: 2
Length of query: 488
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 383
Effective length of database: 11,553,331
Effective search space: 4424925773
Effective search space used: 4424925773
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 158 (65.5 bits)