BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0128400 Os05g0128400|AK100735
         (418 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0128400  Similar to Metal tolerance protein 1 (AtMTP1) ...   701   0.0  
Os08g0422200  Cation efflux protein family protein                 89   9e-18

>Os05g0128400 Similar to Metal tolerance protein 1 (AtMTP1) (Zinc transporter
           ZAT-1) (ZAT1p) [Contains: Metal tolerance protein 1
           short form]
          Length = 418

 Score =  701 bits (1808), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 359/418 (85%), Positives = 359/418 (85%)

Query: 1   MDSHNSAPPQIAEVRMDISSSTSVAAGNKVCRGAACDFSDSSNSSKDARERMASMRKLII 60
           MDSHNSAPPQIAEVRMDISSSTSVAAGNKVCRGAACDFSDSSNSSKDARERMASMRKLII
Sbjct: 1   MDSHNSAPPQIAEVRMDISSSTSVAAGNKVCRGAACDFSDSSNSSKDARERMASMRKLII 60

Query: 61  AVILCIIFMAVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPQQSYGF 120
           AVILCIIFMAVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPQQSYGF
Sbjct: 61  AVILCIIFMAVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPQQSYGF 120

Query: 121 FRIEILGALVSIQLIWLLAGILVYEAIVRLINESGEVQGSLMFAVSAFGLFVNIIMAVLL 180
           FRIEILGALVSIQLIWLLAGILVYEAIVRLINESGEVQGSLMFAVSAFGLFVNIIMAVLL
Sbjct: 121 FRIEILGALVSIQLIWLLAGILVYEAIVRLINESGEVQGSLMFAVSAFGLFVNIIMAVLL 180

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNSITVNLXXXXX 240
                                                           NSITVNL     
Sbjct: 181 GHDHGHGHGHGHGHGHSHDHDHGGSDHDHHHHEDQEHGHVHHHEDGHGNSITVNLHHHPG 240

Query: 241 XXXXXXDAEEPLLKSDAGCDSTQSGAKDAKKARRNINVHSAYLHVLGDSIQSIGVMIGGA 300
                 DAEEPLLKSDAGCDSTQSGAKDAKKARRNINVHSAYLHVLGDSIQSIGVMIGGA
Sbjct: 241 TGHHHHDAEEPLLKSDAGCDSTQSGAKDAKKARRNINVHSAYLHVLGDSIQSIGVMIGGA 300

Query: 301 IIWYKPEWKIIDLICTLIFSVIVLFTTIKMLRNILEVLMESTPREIDATSLENGLRDMDG 360
           IIWYKPEWKIIDLICTLIFSVIVLFTTIKMLRNILEVLMESTPREIDATSLENGLRDMDG
Sbjct: 301 IIWYKPEWKIIDLICTLIFSVIVLFTTIKMLRNILEVLMESTPREIDATSLENGLRDMDG 360

Query: 361 VVAVHELHIWAITVGKVLLACHVTITQDADADQMLDKVIGYIKSEYNISHVTIQIERE 418
           VVAVHELHIWAITVGKVLLACHVTITQDADADQMLDKVIGYIKSEYNISHVTIQIERE
Sbjct: 361 VVAVHELHIWAITVGKVLLACHVTITQDADADQMLDKVIGYIKSEYNISHVTIQIERE 418



>Os08g0422200 Cation efflux protein family protein
          Length = 225

 Score = 88.6 bits (218), Expect = 9e-18,   Method: Compositional matrix adjust.
 Identities = 52/164 (31%), Positives = 92/164 (56%), Gaps = 6/164 (3%)

Query: 256 DAGCDSTQSGAKDAKKARRNINVHSAYLHVLGDSIQSIGVMIGGAIIWYKPEWKIIDLIC 315
           + G  S+++  K   +   + N+   +LHVL D++ S+GV+I   +I YK  W I D IC
Sbjct: 61  NGGLQSSENHNKSRNRHHIDHNMEGIFLHVLADTMGSVGVVISTLLIKYKG-WLIADPIC 119

Query: 316 TLIFSVIVLFTTIKMLRNILEVLMESTPREIDATSLENGLRD---MDGVVAVHELHIWAI 372
           ++  S++++ + + +LRN  E+L++  PR ++   ++  L D   + GV+ VH  H+W +
Sbjct: 120 SVFISIMIVSSVLPLLRNSAEILLQRVPRSLEK-DIKEALDDVMKIKGVIGVHNFHVWNL 178

Query: 373 TVGKVLLACHVTITQDADADQMLDKVIGYIKSEYNISHVTIQIE 416
           T   ++   H+ IT +AD   + +K    I  E  I  +TIQIE
Sbjct: 179 TNTDIVGTFHLHITTEADKSSIREKA-SDIFHEAGIQDLTIQIE 221



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.322    0.135    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 10,598,609
Number of extensions: 371089
Number of successful extensions: 966
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 964
Number of HSP's successfully gapped: 3
Length of query: 418
Length of database: 17,035,801
Length adjustment: 103
Effective length of query: 315
Effective length of database: 11,657,759
Effective search space: 3672194085
Effective search space used: 3672194085
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 157 (65.1 bits)