BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os05g0110700 Os05g0110700|AK102486
         (327 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os05g0110700  Kinetochore-Ndc80 subunit Spc25 family protein      607   e-174
Os01g0973200  Kinetochore-Ndc80 subunit Spc25 family protein      259   2e-69

>Os05g0110700 Kinetochore-Ndc80 subunit Spc25 family protein
          Length = 327

 Score =  607 bits (1564), Expect = e-174,   Method: Compositional matrix adjust.
 Identities = 296/310 (95%), Positives = 296/310 (95%)

Query: 18  ELCRQMAAQLAAYQRGMAVERERQASTSANYRAALLSSRSIARQTISRQEELSGLNLQLR 77
           ELCRQMAAQLAAYQRGMAVERERQASTSANYRAALLSSRSIARQTISRQEELSGLNLQLR
Sbjct: 18  ELCRQMAAQLAAYQRGMAVERERQASTSANYRAALLSSRSIARQTISRQEELSGLNLQLR 77

Query: 78  NLEDGLAEALSVKAGKESKYQLTKETISSTAAINEKLEGMVTDQRNKRDHHAAVISNHLE 137
           NLEDGLAEALSVKAGKESKYQLTKETISSTAAINEKLEGMVTDQRNKRDHHAAVISNHLE
Sbjct: 78  NLEDGLAEALSVKAGKESKYQLTKETISSTAAINEKLEGMVTDQRNKRDHHAAVISNHLE 137

Query: 138 AVEALEAKFIEDETRMKKIEEAVIWYSKFLGFQVVGGEGVKFIFNKIDLQSPDKEYSVTL 197
           AVEALEAKFIEDETRMKKIEEAVIWYSKFLGFQVVGGEGVKFIFNKIDLQSPDKEYSVTL
Sbjct: 138 AVEALEAKFIEDETRMKKIEEAVIWYSKFLGFQVVGGEGVKFIFNKIDLQSPDKEYSVTL 197

Query: 198 KLAKDRYNLLQCDPSIKDSEELMKDLNLTNDLFKFVRIVRERFQAEAATVNGGLLMSSVV 257
           KLAKDRYNLLQCDPSIKDSEELMKDLNLTNDLFKFVRIVRERFQAEAATVNGGLLMSSVV
Sbjct: 198 KLAKDRYNLLQCDPSIKDSEELMKDLNLTNDLFKFVRIVRERFQAEAATVNGGLLMSSVV 257

Query: 258 CPDASSIPVSPPMLMPLDSRTENVLDKSLSQSKNKGRNLPSKRGXXXXXXXXXXXXXXIV 317
           CPDASSIPVSPPMLMPLDSRTENVLDKSLSQSKNKGRNLPSKRG              IV
Sbjct: 258 CPDASSIPVSPPMLMPLDSRTENVLDKSLSQSKNKGRNLPSKRGAAALSAASPGSAVSIV 317

Query: 318 RRSPHFVGIR 327
           RRSPHFVGIR
Sbjct: 318 RRSPHFVGIR 327



>Os01g0973200 Kinetochore-Ndc80 subunit Spc25 family protein
          Length = 359

 Score =  259 bits (661), Expect = 2e-69,   Method: Compositional matrix adjust.
 Identities = 153/300 (51%), Positives = 208/300 (69%), Gaps = 21/300 (7%)

Query: 18  ELCRQMAAQLAAYQRGMAVERERQASTSANYRAALLSSRSIARQTISRQEELSGLNLQLR 77
           EL R+MAAQ  A++R +A  RER  + ++ + AALLS+RS++  TIS++E+ + L  QLR
Sbjct: 13  ELRRRMAAQCLAFERQIADGRERTKAAASAFSAALLSARSLSNHTISQREKSNQLKDQLR 72

Query: 78  NLEDGLAEALS-------------VKAGKESKYQLTKETISSTAAINEKLEGMVTDQRNK 124
            LE   A+ALS             V+   ++KY LT ++I++  A N++L  +VTD+R  
Sbjct: 73  KLEADFAQALSSHGRSETLQTSSAVQGSNKTKYDLTGQSITNAIATNDQLSCLVTDKRAS 132

Query: 125 RDHHAAVISNHLEAVEALEAKFIEDETRMKKIEEAVIWYSKFLGFQVVGGEGVKFIFNKI 184
           RD +A VIS+ LEA+EALEAK   D    K ++EA +WY KFLGFQVVGGEGVKF+F+KI
Sbjct: 133 RDEYANVISSQLEAIEALEAK--TDAAGKKNLDEAFMWYKKFLGFQVVGGEGVKFVFSKI 190

Query: 185 DLQSPDKEYSVTLKLAKDRYNLLQCDPSIKDSEELMKDLNLTNDLFKFVRIVRERFQAEA 244
           D+Q+PD EYS  +KL KDRYNLLQC P +KDSEEL+KDLN +NDLFKFVRI+RERFQ  A
Sbjct: 191 DIQNPDNEYSFCIKLNKDRYNLLQCTPFLKDSEELVKDLNCSNDLFKFVRIMRERFQ--A 248

Query: 245 ATVNGGLLMSSVVCPD-ASSIPVSPPMLMPLDSRTENVLDKSLSQSKNKGRNL--PSKRG 301
           A +N G L +S +CPD +SSI  S P  + +D+  E+    S S S+++ +N   P+KRG
Sbjct: 249 AAIN-GFLPASSLCPDMSSSITDSSPPALSIDTGRESTTTTSQSHSRSRAKNQDNPTKRG 307



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.129    0.349 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,298,450
Number of extensions: 289524
Number of successful extensions: 1090
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1087
Number of HSP's successfully gapped: 2
Length of query: 327
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 226
Effective length of database: 11,762,187
Effective search space: 2658254262
Effective search space used: 2658254262
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 156 (64.7 bits)