BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0682000 Os04g0682000|AK069012
         (478 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0682000  Similar to Autophagy 4a                             898   0.0  
AK110731                                                          149   5e-36
Os03g0391000  Peptidase C54 family protein                        102   7e-22

>Os04g0682000 Similar to Autophagy 4a
          Length = 478

 Score =  898 bits (2320), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 438/478 (91%), Positives = 438/478 (91%)

Query: 1   MTSLPDRGVSSSSSDPLCEGNIAPCSSSSEQKEDCSLKQSKTSILSCVFNSPFNIFEAHQ 60
           MTSLPDRGVSSSSSDPLCEGNIAPCSSSSEQKEDCSLKQSKTSILSCVFNSPFNIFEAHQ
Sbjct: 1   MTSLPDRGVSSSSSDPLCEGNIAPCSSSSEQKEDCSLKQSKTSILSCVFNSPFNIFEAHQ 60

Query: 61  DXXXXXXXXXXXXXXXXXRVLRRIVCSGSMWRFLGTSKVLTSSDVWFLGKCYKLXXXXXX 120
           D                 RVLRRIVCSGSMWRFLGTSKVLTSSDVWFLGKCYKL      
Sbjct: 61  DSSANKSPKSSSGSYDWSRVLRRIVCSGSMWRFLGTSKVLTSSDVWFLGKCYKLSSEESS 120

Query: 121 XXXXXXXGHATFLEDFSSRIWITYRRGFDAISDSKYTSDVNWGCMVRSSQMLVAQALIFH 180
                  GHATFLEDFSSRIWITYRRGFDAISDSKYTSDVNWGCMVRSSQMLVAQALIFH
Sbjct: 121 SDSDSESGHATFLEDFSSRIWITYRRGFDAISDSKYTSDVNWGCMVRSSQMLVAQALIFH 180

Query: 181 HLGRSWRRPLEKPYNPEYIGILHMFGDSEACAFSIHNLLQAGNSYGLAAGSWVGPYAMCR 240
           HLGRSWRRPLEKPYNPEYIGILHMFGDSEACAFSIHNLLQAGNSYGLAAGSWVGPYAMCR
Sbjct: 181 HLGRSWRRPLEKPYNPEYIGILHMFGDSEACAFSIHNLLQAGNSYGLAAGSWVGPYAMCR 240

Query: 241 AWQTLVRTNREQHEVVDGNESFPMALYVVSGDEDGERGGAPVVCIDVAAQLCCDFNKGQS 300
           AWQTLVRTNREQHEVVDGNESFPMALYVVSGDEDGERGGAPVVCIDVAAQLCCDFNKGQS
Sbjct: 241 AWQTLVRTNREQHEVVDGNESFPMALYVVSGDEDGERGGAPVVCIDVAAQLCCDFNKGQS 300

Query: 301 TWSPIXXXXXXXXXXDKINPRYIPLLKETFTFPQSLGILGGKPGTSTYIAGVQDDRALYL 360
           TWSPI          DKINPRYIPLLKETFTFPQSLGILGGKPGTSTYIAGVQDDRALYL
Sbjct: 301 TWSPILLLVPLVLGLDKINPRYIPLLKETFTFPQSLGILGGKPGTSTYIAGVQDDRALYL 360

Query: 361 DPHEVQMAVDIAADNIEADTSSYHCSTVRDLALDLIDPSLAIGFYCRDKDDFDDFCSRAT 420
           DPHEVQMAVDIAADNIEADTSSYHCSTVRDLALDLIDPSLAIGFYCRDKDDFDDFCSRAT
Sbjct: 361 DPHEVQMAVDIAADNIEADTSSYHCSTVRDLALDLIDPSLAIGFYCRDKDDFDDFCSRAT 420

Query: 421 ELVDKANGAPLFTVVQSVQPSKQMYNQDDVLGISGDGNINVEDLDASGETGEEEWQIL 478
           ELVDKANGAPLFTVVQSVQPSKQMYNQDDVLGISGDGNINVEDLDASGETGEEEWQIL
Sbjct: 421 ELVDKANGAPLFTVVQSVQPSKQMYNQDDVLGISGDGNINVEDLDASGETGEEEWQIL 478



>AK110731 
          Length = 403

 Score =  149 bits (375), Expect = 5e-36,   Method: Compositional matrix adjust.
 Identities = 109/347 (31%), Positives = 153/347 (44%), Gaps = 55/347 (15%)

Query: 132 FLEDFSSRIWITYRRGFDAISDSK-----------------------YTSDVNWGCMVRS 168
           FL+DF SRIW+TYR  F  I  +K                       +TSD  WGCM+RS
Sbjct: 73  FLDDFESRIWMTYRSNFTPIPRNKTPEATSSMTLGVRLRSQLMDSQGFTSDTGWGCMIRS 132

Query: 169 SQMLVAQALIFHHLGRSWRRPLEKPYNPEYIGILHMFGDSEACAFSIHNLLQAG-NSYGL 227
            Q L+A  L    LGR WRR        E   +L MF D     FS+H  +Q G  S G 
Sbjct: 133 GQSLLANTLSMIQLGRDWRR---GSSPEEESKLLAMFADHPDSPFSVHRFVQRGAESCGK 189

Query: 228 AAGSWVGPYAMCRAWQTLVRTNREQHEVVDGNESFPMALYVVSGDEDGERGGAPVVCIDV 287
             G W GP A  R  Q L       HE     ES  + +Y+ +   D        V  D 
Sbjct: 190 YPGEWFGPSATARCIQLL------SHEC----ESPKLRVYITNNTSD--------VYEDE 231

Query: 288 AAQLCCDFNKGQSTWSPIXXXXXXXXXXDKINPRYIPLLKETFTFPQSLGILGGKPGTST 347
            A+L  D N GQ    P+          +++   Y   L+    +PQS+GI GG+P  S 
Sbjct: 232 VARLGRDEN-GQPV--PVLVLLGLRLGIEQVTSVYWDGLRAALQYPQSVGIAGGRPSASH 288

Query: 348 YIAGVQDDRALYLDPHEVQMAVDIAADNI---EADTSSYHCSTVRDLALDLIDPSLAIGF 404
           Y  GVQD    +LDPH  + A+ +   +      D  + H   +R + +  +DPS+ IGF
Sbjct: 289 YFVGVQDSHLFFLDPHSTRPALPVPQSSKVYSREDLDTCHTRRLRRIHIKDMDPSMLIGF 348

Query: 405 YCRDKDDFDDFCSRATELVDKANGAPLFTVVQSVQPSKQMYNQDDVL 451
             RD+ D+ D+  R    V    G P+  ++ S  P+++   + + L
Sbjct: 349 LIRDESDWKDWKQR----VQSTPGKPIIHILASGAPTEEGMERMEAL 391



>Os03g0391000 Peptidase C54 family protein
          Length = 81

 Score =  102 bits (254), Expect = 7e-22,   Method: Composition-based stats.
 Identities = 45/47 (95%), Positives = 46/47 (97%)

Query: 321 RYIPLLKETFTFPQSLGILGGKPGTSTYIAGVQDDRALYLDPHEVQM 367
           RYIPLLKET TFPQSLGILGGKPGTSTYIAGVQDDRALYLDPHEVQ+
Sbjct: 10  RYIPLLKETLTFPQSLGILGGKPGTSTYIAGVQDDRALYLDPHEVQL 56



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.135    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 16,174,465
Number of extensions: 643088
Number of successful extensions: 1297
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 1291
Number of HSP's successfully gapped: 3
Length of query: 478
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 373
Effective length of database: 11,553,331
Effective search space: 4309392463
Effective search space used: 4309392463
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 158 (65.5 bits)