BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0669700 Os04g0669700|Os04g0669700
         (245 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0669700  Phospholipase/Carboxylesterase family protein       507   e-144
Os04g0669500  Phospholipase/Carboxylesterase family protein       237   6e-63
Os04g0669600  Phospholipase/Carboxylesterase family protein       214   4e-56
Os01g0612500  Phospholipase/Carboxylesterase family protein        77   1e-14
Os01g0175000  Phospholipase/Carboxylesterase family protein        77   2e-14

>Os04g0669700 Phospholipase/Carboxylesterase family protein
          Length = 245

 Score =  507 bits (1306), Expect = e-144,   Method: Compositional matrix adjust.
 Identities = 245/245 (100%), Positives = 245/245 (100%)

Query: 1   MEPATSRAPRSRFVVWLHGLGDTGRANEFLADSFPTTAAFADARWAFPTAPTAPVTCNRG 60
           MEPATSRAPRSRFVVWLHGLGDTGRANEFLADSFPTTAAFADARWAFPTAPTAPVTCNRG
Sbjct: 1   MEPATSRAPRSRFVVWLHGLGDTGRANEFLADSFPTTAAFADARWAFPTAPTAPVTCNRG 60

Query: 61  MLMPSWFDIHDAPITSVSVRDEEDVLRAVQSVHAMIDREIAAGTNPQDVFVFGLSQGGAL 120
           MLMPSWFDIHDAPITSVSVRDEEDVLRAVQSVHAMIDREIAAGTNPQDVFVFGLSQGGAL
Sbjct: 61  MLMPSWFDIHDAPITSVSVRDEEDVLRAVQSVHAMIDREIAAGTNPQDVFVFGLSQGGAL 120

Query: 121 GIASVLLHPKTLGGCAVFSGFLPFNSSFAVRVTAQAKKTPVLWIHGQAGSLIPIKEGRDG 180
           GIASVLLHPKTLGGCAVFSGFLPFNSSFAVRVTAQAKKTPVLWIHGQAGSLIPIKEGRDG
Sbjct: 121 GIASVLLHPKTLGGCAVFSGFLPFNSSFAVRVTAQAKKTPVLWIHGQAGSLIPIKEGRDG 180

Query: 181 IKFLRGLGMSCEFKVYDRLGHSLEYYELDYCQRWVEKILHRSGREGLIRRVSRNIFLCSN 240
           IKFLRGLGMSCEFKVYDRLGHSLEYYELDYCQRWVEKILHRSGREGLIRRVSRNIFLCSN
Sbjct: 181 IKFLRGLGMSCEFKVYDRLGHSLEYYELDYCQRWVEKILHRSGREGLIRRVSRNIFLCSN 240

Query: 241 LFNSS 245
           LFNSS
Sbjct: 241 LFNSS 245



>Os04g0669500 Phospholipase/Carboxylesterase family protein
          Length = 266

 Score =  237 bits (604), Expect = 6e-63,   Method: Compositional matrix adjust.
 Identities = 112/210 (53%), Positives = 153/210 (72%), Gaps = 1/210 (0%)

Query: 13  FVVWLHGLGDTGRANEFLADSFPTTAAFADARWAFPTAPTAPVTCNRGMLMPSWFDIHDA 72
           FV+WLHGLGD+G ANE + + F +   F   +WAFP+AP +PV+CN G +MPSWFDIH+ 
Sbjct: 53  FVLWLHGLGDSGPANEPIRNFF-SAPEFRLTKWAFPSAPNSPVSCNHGAVMPSWFDIHEL 111

Query: 73  PITSVSVRDEEDVLRAVQSVHAMIDREIAAGTNPQDVFVFGLSQGGALGIASVLLHPKTL 132
           P++S S +D+  VL+AV++VHAMID+E+A G  P+++FV G SQGGAL +ASVLL+PKTL
Sbjct: 112 PMSSGSPQDDSGVLKAVENVHAMIDKEVADGIPPENIFVCGFSQGGALTLASVLLYPKTL 171

Query: 133 GGCAVFSGFLPFNSSFAVRVTAQAKKTPVLWIHGQAGSLIPIKEGRDGIKFLRGLGMSCE 192
           GG AVFSG+LPF SS   R++ +A+KTP+LW HG A +++  + G+ G  FL+  G SCE
Sbjct: 172 GGGAVFSGWLPFGSSVTERISPEARKTPILWSHGIADNVVLFEAGQAGPPFLQNAGFSCE 231

Query: 193 FKVYDRLGHSLEYYELDYCQRWVEKILHRS 222
           FK Y  LGHS+   EL   + W++  L  S
Sbjct: 232 FKAYPGLGHSISKEELYSLESWIKNHLKAS 261



>Os04g0669600 Phospholipase/Carboxylesterase family protein
          Length = 235

 Score =  214 bits (546), Expect = 4e-56,   Method: Compositional matrix adjust.
 Identities = 101/208 (48%), Positives = 141/208 (67%)

Query: 14  VVWLHGLGDTGRANEFLADSFPTTAAFADARWAFPTAPTAPVTCNRGMLMPSWFDIHDAP 73
           V+WLHG G TG  +      +      A  R++FPTAPT+ + C  G ++ +WF I + P
Sbjct: 12  VLWLHGSGQTGEESRAQVAPYFAAPELASVRFSFPTAPTSSIPCYGGEVITAWFAIPEVP 71

Query: 74  ITSVSVRDEEDVLRAVQSVHAMIDREIAAGTNPQDVFVFGLSQGGALGIASVLLHPKTLG 133
           IT+ + RDE++VL+AV+ VH M+D E+AAGT+P ++FV GLSQGGAL IASVLL+P TLG
Sbjct: 72  ITARTARDEKEVLKAVERVHEMLDGEVAAGTSPSNIFVCGLSQGGALAIASVLLYPMTLG 131

Query: 134 GCAVFSGFLPFNSSFAVRVTAQAKKTPVLWIHGQAGSLIPIKEGRDGIKFLRGLGMSCEF 193
           GC VFSG LP + +FA  + ++A+KTPVLW HG A  ++  + G  G  FL+ +GM CEF
Sbjct: 132 GCVVFSGSLPLSKTFAESIPSEARKTPVLWFHGMADGVVLFEAGHAGCAFLQEIGMHCEF 191

Query: 194 KVYDRLGHSLEYYELDYCQRWVEKILHR 221
           K Y  LGH+L   EL Y ++W++  L +
Sbjct: 192 KAYPALGHTLVDEELQYFRQWIKDRLSQ 219



>Os01g0612500 Phospholipase/Carboxylesterase family protein
          Length = 243

 Score = 77.0 bits (188), Expect = 1e-14,   Method: Compositional matrix adjust.
 Identities = 64/209 (30%), Positives = 93/209 (44%), Gaps = 32/209 (15%)

Query: 14  VVWLHGLGDTGRANEFLADSFPTTAAFADARWAFPTAPTAPVTCNRGMLMPSWFDIHDAP 73
           +VWLHGLGD G +   L DS P      + +W  PTAPT PV    G    +WFD+ D  
Sbjct: 34  IVWLHGLGDNGASWSQLLDSLP----LPNIKWICPTAPTRPVAAFGGFPCTAWFDVED-- 87

Query: 74  ITSVSVRDEEDVLRAVQSVHAMIDREIAAGTNPQDVF--VFGLSQGGALG---------- 121
            TSV  RD+ + L A  +  A +       + P DV   + G S G A            
Sbjct: 88  -TSVDGRDDIEGLDASAAHVANL-----LSSEPSDVKLGIGGFSMGAAAALHSAACYAHG 141

Query: 122 -IASVLLHPKTLGGCAVFSGFLPFNSSFAVRVTA------QAKKTPVLWIHGQAGSLIPI 174
              + + +P TL      SG+LP + +   ++ +      +A   P+L  HG+A  ++  
Sbjct: 142 RFTNGVAYPVTLSAVIGLSGWLPCSRTLKSKMDSSQTALRRAGALPILLSHGRADEVVTY 201

Query: 175 KEGRDGIKFLRGLGMS-CEFKVYDRLGHS 202
           + G     FLRG G     FK Y+   H+
Sbjct: 202 RNGEKSADFLRGSGFQYLNFKPYNGYRHT 230



>Os01g0175000 Phospholipase/Carboxylesterase family protein
          Length = 301

 Score = 76.6 bits (187), Expect = 2e-14,   Method: Compositional matrix adjust.
 Identities = 61/208 (29%), Positives = 89/208 (42%), Gaps = 32/208 (15%)

Query: 14  VVWLHGLGDTGRANEFLADSFPTTAAFADARWAFPTAPTAPVTCNRGMLMPSWFDIHDAP 73
           +VWLHGLGD G +   L ++ P      + +W  PTAPT PV    G    +WFD+ D  
Sbjct: 97  IVWLHGLGDNGASWSQLLETLP----LPNIKWICPTAPTRPVAVFGGFPSTAWFDVADLS 152

Query: 74  ITSVSVRDEEDVLRAVQSVHAMIDREIAAGTNPQDVF--VFGLSQGGALGIASVLL---- 127
                  D  D +  + +  A +   ++  T P D+   V G S G A  + S       
Sbjct: 153 ------EDAPDDVEGLDASAAHVANLLS--TEPADIKLGVGGFSMGAATALYSATCYAHG 204

Query: 128 -------HPKTLGGCAVFSGFLPFNSSFAVRVTA------QAKKTPVLWIHGQAGSLIPI 174
                  +P  L      SG+LP   S   ++ +      +A   P+L  HG+A  ++  
Sbjct: 205 KYGNGNPYPVNLSVSVGLSGWLPCARSLKNKIESSQEAAQKASSIPLLLCHGKADDVVLY 264

Query: 175 KEGRDGIKFLRGLGMS-CEFKVYDRLGH 201
           K G      L+  G S   FK Y+RLGH
Sbjct: 265 KHGEKSADALKTTGFSNVVFKSYNRLGH 292



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.324    0.138    0.433 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,042,423
Number of extensions: 376787
Number of successful extensions: 1034
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 1029
Number of HSP's successfully gapped: 5
Length of query: 245
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 147
Effective length of database: 11,918,829
Effective search space: 1752067863
Effective search space used: 1752067863
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 154 (63.9 bits)