BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0669600 Os04g0669600|AK110767
         (235 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0669600  Phospholipase/Carboxylesterase family protein       481   e-136
Os04g0669500  Phospholipase/Carboxylesterase family protein       234   3e-62
Os04g0669700  Phospholipase/Carboxylesterase family protein       214   3e-56
Os01g0175000  Phospholipase/Carboxylesterase family protein        76   2e-14
Os01g0612500  Phospholipase/Carboxylesterase family protein        72   5e-13

>Os04g0669600 Phospholipase/Carboxylesterase family protein
          Length = 235

 Score =  481 bits (1237), Expect = e-136,   Method: Compositional matrix adjust.
 Identities = 235/235 (100%), Positives = 235/235 (100%)

Query: 1   MGMAAAGGYGRVLWLHGSGQTGEESRAQVAPYFAAPELASVRFSFPTAPTSSIPCYGGEV 60
           MGMAAAGGYGRVLWLHGSGQTGEESRAQVAPYFAAPELASVRFSFPTAPTSSIPCYGGEV
Sbjct: 1   MGMAAAGGYGRVLWLHGSGQTGEESRAQVAPYFAAPELASVRFSFPTAPTSSIPCYGGEV 60

Query: 61  ITAWFAIPEVPITARTARDEKEVLKAVERVHEMLDGEVAAGTSPSNIFVCGLSQGGALAI 120
           ITAWFAIPEVPITARTARDEKEVLKAVERVHEMLDGEVAAGTSPSNIFVCGLSQGGALAI
Sbjct: 61  ITAWFAIPEVPITARTARDEKEVLKAVERVHEMLDGEVAAGTSPSNIFVCGLSQGGALAI 120

Query: 121 ASVLLYPMTLGGCVVFSGSLPLSKTFAESIPSEARKTPVLWFHGMADGVVLFEAGHAGCA 180
           ASVLLYPMTLGGCVVFSGSLPLSKTFAESIPSEARKTPVLWFHGMADGVVLFEAGHAGCA
Sbjct: 121 ASVLLYPMTLGGCVVFSGSLPLSKTFAESIPSEARKTPVLWFHGMADGVVLFEAGHAGCA 180

Query: 181 FLQEIGMHCEFKAYPALGHTLVDEELQYFRQWIKDRLSQGTGVPVPSLSDKMDLQ 235
           FLQEIGMHCEFKAYPALGHTLVDEELQYFRQWIKDRLSQGTGVPVPSLSDKMDLQ
Sbjct: 181 FLQEIGMHCEFKAYPALGHTLVDEELQYFRQWIKDRLSQGTGVPVPSLSDKMDLQ 235



>Os04g0669500 Phospholipase/Carboxylesterase family protein
          Length = 266

 Score =  234 bits (598), Expect = 3e-62,   Method: Compositional matrix adjust.
 Identities = 114/206 (55%), Positives = 141/206 (68%), Gaps = 1/206 (0%)

Query: 12  VLWLHGSGQTGEESRAQVAPYFAAPELASVRFSFPTAPTSSIPCYGGEVITAWFAIPEVP 71
           VLWLHG G +G  +   +  +F+APE    +++FP+AP S + C  G V+ +WF I E+P
Sbjct: 54  VLWLHGLGDSGPANEP-IRNFFSAPEFRLTKWAFPSAPNSPVSCNHGAVMPSWFDIHELP 112

Query: 72  ITARTARDEKEVLKAVERVHEMLDGEVAAGTSPSNIFVCGLSQGGALAIASVLLYPMTLG 131
           +++ + +D+  VLKAVE VH M+D EVA G  P NIFVCG SQGGAL +ASVLLYP TLG
Sbjct: 113 MSSGSPQDDSGVLKAVENVHAMIDKEVADGIPPENIFVCGFSQGGALTLASVLLYPKTLG 172

Query: 132 GCVVFSGSLPLSKTFAESIPSEARKTPVLWFHGMADGVVLFEAGHAGCAFLQEIGMHCEF 191
           G  VFSG LP   +  E I  EARKTP+LW HG+AD VVLFEAG AG  FLQ  G  CEF
Sbjct: 173 GGAVFSGWLPFGSSVTERISPEARKTPILWSHGIADNVVLFEAGQAGPPFLQNAGFSCEF 232

Query: 192 KAYPALGHTLVDEELQYFRQWIKDRL 217
           KAYP LGH++  EEL     WIK+ L
Sbjct: 233 KAYPGLGHSISKEELYSLESWIKNHL 258



>Os04g0669700 Phospholipase/Carboxylesterase family protein
          Length = 245

 Score =  214 bits (546), Expect = 3e-56,   Method: Compositional matrix adjust.
 Identities = 101/208 (48%), Positives = 141/208 (67%)

Query: 12  VLWLHGSGQTGEESRAQVAPYFAAPELASVRFSFPTAPTSSIPCYGGEVITAWFAIPEVP 71
           V+WLHG G TG  +      +      A  R++FPTAPT+ + C  G ++ +WF I + P
Sbjct: 14  VVWLHGLGDTGRANEFLADSFPTTAAFADARWAFPTAPTAPVTCNRGMLMPSWFDIHDAP 73

Query: 72  ITARTARDEKEVLKAVERVHEMLDGEVAAGTSPSNIFVCGLSQGGALAIASVLLYPMTLG 131
           IT+ + RDE++VL+AV+ VH M+D E+AAGT+P ++FV GLSQGGAL IASVLL+P TLG
Sbjct: 74  ITSVSVRDEEDVLRAVQSVHAMIDREIAAGTNPQDVFVFGLSQGGALGIASVLLHPKTLG 133

Query: 132 GCVVFSGSLPLSKTFAESIPSEARKTPVLWFHGMADGVVLFEAGHAGCAFLQEIGMHCEF 191
           GC VFSG LP + +FA  + ++A+KTPVLW HG A  ++  + G  G  FL+ +GM CEF
Sbjct: 134 GCAVFSGFLPFNSSFAVRVTAQAKKTPVLWIHGQAGSLIPIKEGRDGIKFLRGLGMSCEF 193

Query: 192 KAYPALGHTLVDEELQYFRQWIKDRLSQ 219
           K Y  LGH+L   EL Y ++W++  L +
Sbjct: 194 KVYDRLGHSLEYYELDYCQRWVEKILHR 221



>Os01g0175000 Phospholipase/Carboxylesterase family protein
          Length = 301

 Score = 76.3 bits (186), Expect = 2e-14,   Method: Compositional matrix adjust.
 Identities = 66/217 (30%), Positives = 98/217 (45%), Gaps = 34/217 (15%)

Query: 12  VLWLHGSGQTGEESRAQVAPYFAAPELASVRFSFPTAPTSSIPCYGGEVITAWFAIPEVP 71
           ++WLHG G  G    A  +       L ++++  PTAPT  +  +GG   TAWF + ++ 
Sbjct: 97  IVWLHGLGDNG----ASWSQLLETLPLPNIKWICPTAPTRPVAVFGGFPSTAWFDVADL- 151

Query: 72  ITARTARDEKEVLKA-VERVHEMLDGEVAAGTSPSNIF--VCGLSQGGALAIASVLL--- 125
             +  A D+ E L A    V  +L       T P++I   V G S G A A+ S      
Sbjct: 152 --SEDAPDDVEGLDASAAHVANLLS------TEPADIKLGVGGFSMGAATALYSATCYAH 203

Query: 126 --------YPMTLGGCVVFSGSLPLSKTFAESIPS------EARKTPVLWFHGMADGVVL 171
                   YP+ L   V  SG LP +++    I S      +A   P+L  HG AD VVL
Sbjct: 204 GKYGNGNPYPVNLSVSVGLSGWLPCARSLKNKIESSQEAAQKASSIPLLLCHGKADDVVL 263

Query: 172 FEAGHAGCAFLQEIGM-HCEFKAYPALGHTLVDEELQ 207
           ++ G      L+  G  +  FK+Y  LGH  V  +++
Sbjct: 264 YKHGEKSADALKTTGFSNVVFKSYNRLGHYTVPNKIE 300



>Os01g0612500 Phospholipase/Carboxylesterase family protein
          Length = 243

 Score = 71.6 bits (174), Expect = 5e-13,   Method: Compositional matrix adjust.
 Identities = 65/212 (30%), Positives = 93/212 (43%), Gaps = 34/212 (16%)

Query: 12  VLWLHGSGQTGEESRAQVAPYFAAPELASVRFSFPTAPTSSIPCYGGEVITAWFAIPEVP 71
           ++WLHG G  G    A  +    +  L ++++  PTAPT  +  +GG   TAWF   +V 
Sbjct: 34  IVWLHGLGDNG----ASWSQLLDSLPLPNIKWICPTAPTRPVAAFGGFPCTAWF---DVE 86

Query: 72  ITARTARDEKEVLKA-VERVHEMLDGEVAAGTSPSNIF--VCGLSQGGALA--------- 119
            T+   RD+ E L A    V  +L  E      PS++   + G S G A A         
Sbjct: 87  DTSVDGRDDIEGLDASAAHVANLLSSE------PSDVKLGIGGFSMGAAAALHSAACYAH 140

Query: 120 --IASVLLYPMTLGGCVVFSGSLPLSKTFAESIPS------EARKTPVLWFHGMADGVVL 171
               + + YP+TL   +  SG LP S+T    + S       A   P+L  HG AD VV 
Sbjct: 141 GRFTNGVAYPVTLSAVIGLSGWLPCSRTLKSKMDSSQTALRRAGALPILLSHGRADEVVT 200

Query: 172 FEAGHAGCAFLQEIGM-HCEFKAYPALGHTLV 202
           +  G     FL+  G  +  FK Y    HT +
Sbjct: 201 YRNGEKSADFLRGSGFQYLNFKPYNGYRHTYL 232



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.136    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,918,832
Number of extensions: 379798
Number of successful extensions: 948
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 941
Number of HSP's successfully gapped: 5
Length of query: 235
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 137
Effective length of database: 11,918,829
Effective search space: 1632879573
Effective search space used: 1632879573
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 154 (63.9 bits)