BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0623400 Os04g0623400|AK110887
(282 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os04g0623400 Exonuclease domain containing protein 584 e-167
Os03g0301400 Exonuclease domain containing protein 208 4e-54
>Os04g0623400 Exonuclease domain containing protein
Length = 282
Score = 584 bits (1505), Expect = e-167, Method: Compositional matrix adjust.
Identities = 282/282 (100%), Positives = 282/282 (100%)
Query: 1 MSSILRSIQLRNSIWSSFPVRFLKQQAGLSTVELLDPRSCGKRHFTTQVQEHGKEVAAAA 60
MSSILRSIQLRNSIWSSFPVRFLKQQAGLSTVELLDPRSCGKRHFTTQVQEHGKEVAAAA
Sbjct: 1 MSSILRSIQLRNSIWSSFPVRFLKQQAGLSTVELLDPRSCGKRHFTTQVQEHGKEVAAAA 60
Query: 61 TILVFDLETTGYFHKDHRIVEFALCDLSGGKNSTFETLVNPERTVPDYVEHLIKIGTDLV 120
TILVFDLETTGYFHKDHRIVEFALCDLSGGKNSTFETLVNPERTVPDYVEHLIKIGTDLV
Sbjct: 61 TILVFDLETTGYFHKDHRIVEFALCDLSGGKNSTFETLVNPERTVPDYVEHLIKIGTDLV 120
Query: 121 CRPGIPRFSDVIPLLLAFVRSRQAPGKPVLWVAHNAKQFDARFLAQEFDRCSAPLPADWL 180
CRPGIPRFSDVIPLLLAFVRSRQAPGKPVLWVAHNAKQFDARFLAQEFDRCSAPLPADWL
Sbjct: 121 CRPGIPRFSDVIPLLLAFVRSRQAPGKPVLWVAHNAKQFDARFLAQEFDRCSAPLPADWL 180
Query: 181 FFDTLLLAKKMVKAEGKKRRTSLEALREHYGIVSHDDAHRAMRDVMILSQVFQKMTFDLK 240
FFDTLLLAKKMVKAEGKKRRTSLEALREHYGIVSHDDAHRAMRDVMILSQVFQKMTFDLK
Sbjct: 181 FFDTLLLAKKMVKAEGKKRRTSLEALREHYGIVSHDDAHRAMRDVMILSQVFQKMTFDLK 240
Query: 241 LTNEELINEAMKLPFHVFCDSVCLLFLWTSFEQQSEPEREEI 282
LTNEELINEAMKLPFHVFCDSVCLLFLWTSFEQQSEPEREEI
Sbjct: 241 LTNEELINEAMKLPFHVFCDSVCLLFLWTSFEQQSEPEREEI 282
>Os03g0301400 Exonuclease domain containing protein
Length = 313
Score = 208 bits (529), Expect = 4e-54, Method: Compositional matrix adjust.
Identities = 102/202 (50%), Positives = 138/202 (68%), Gaps = 3/202 (1%)
Query: 53 GKEVAAAATILVFDLETTGYFHKDHRIVEFALCDLSGGKNSTFETLVNPERTVPDYVEHL 112
G + ATILVFD+ETTG+ + RI+EFA+ DL GGKNST +TL+NPER + + H
Sbjct: 104 GVKTDWPATILVFDIETTGFSRRYERIIEFAVRDLMGGKNSTIQTLINPEREIKNAYVH- 162
Query: 113 IKIGTDLVCRPGIPRFSDVIPLLLAFVRSRQAPGKPVLWVAHNAKQFDARFLAQEFDRCS 172
I + +VC+P IPRF + IPLLL +V SRQ KPVLWVAHN + FD FL EF R
Sbjct: 163 -GISSSMVCKPDIPRFREFIPLLLQYVLSRQMADKPVLWVAHNGRSFDVPFLMYEFQRSK 221
Query: 173 APLPADWLFFDTLLLAKKMVKAEGKK-RRTSLEALREHYGIVSHDDAHRAMRDVMILSQV 231
+P DWLF DTL +A++++ ++G+K + SL+ LREHY I AHRAM+DV+ L V
Sbjct: 222 IEMPGDWLFVDTLPIARQLIGSDGEKLKSVSLDNLREHYKIPLAGSAHRAMQDVITLCYV 281
Query: 232 FQKMTFDLKLTNEELINEAMKL 253
QK+TF+LKLT +L+ + ++
Sbjct: 282 LQKLTFELKLTVPQLLERSFRV 303
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.324 0.137 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,605,316
Number of extensions: 379519
Number of successful extensions: 911
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 909
Number of HSP's successfully gapped: 2
Length of query: 282
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 182
Effective length of database: 11,814,401
Effective search space: 2150220982
Effective search space used: 2150220982
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 155 (64.3 bits)