BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0612600 Os04g0612600|AK060707
         (287 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0612600  Similar to Coatomer-like protein, epsilon subunit   588   e-168
Os04g0644700  Coatomer epsilon subunit family protein             406   e-113

>Os04g0612600 Similar to Coatomer-like protein, epsilon subunit
          Length = 287

 Score =  588 bits (1517), Expect = e-168,   Method: Compositional matrix adjust.
 Identities = 287/287 (100%), Positives = 287/287 (100%)

Query: 1   MASPDLLFNLRNLFYLGAYQAAINNSDVPGLDADAAAERDAIVFRSYVALGSYQLVISEI 60
           MASPDLLFNLRNLFYLGAYQAAINNSDVPGLDADAAAERDAIVFRSYVALGSYQLVISEI
Sbjct: 1   MASPDLLFNLRNLFYLGAYQAAINNSDVPGLDADAAAERDAIVFRSYVALGSYQLVISEI 60

Query: 61  DSSAATSLQAVKLLALYLSGDKESAIVSLKEWLSDSAVGSNPVLRLIAGIIFMHEQDYTE 120
           DSSAATSLQAVKLLALYLSGDKESAIVSLKEWLSDSAVGSNPVLRLIAGIIFMHEQDYTE
Sbjct: 61  DSSAATSLQAVKLLALYLSGDKESAIVSLKEWLSDSAVGSNPVLRLIAGIIFMHEQDYTE 120

Query: 121 ALKHTHSGGTLDLHALNVQIFIKMHRSDYAEKQLKIMQQIDEDHTLTQLANAWLDIAVGG 180
           ALKHTHSGGTLDLHALNVQIFIKMHRSDYAEKQLKIMQQIDEDHTLTQLANAWLDIAVGG
Sbjct: 121 ALKHTHSGGTLDLHALNVQIFIKMHRSDYAEKQLKIMQQIDEDHTLTQLANAWLDIAVGG 180

Query: 181 SKIREAYLIFQDFAEKYPMTGMVLNGKAVCCMHMGSFDEAETLLLEALNKDAKDPETLAN 240
           SKIREAYLIFQDFAEKYPMTGMVLNGKAVCCMHMGSFDEAETLLLEALNKDAKDPETLAN
Sbjct: 181 SKIREAYLIFQDFAEKYPMTGMVLNGKAVCCMHMGSFDEAETLLLEALNKDAKDPETLAN 240

Query: 241 LIVCNLHLGKPSSRYLSQLKLSHPDHVLVKRAVSAEDNFERALQAVA 287
           LIVCNLHLGKPSSRYLSQLKLSHPDHVLVKRAVSAEDNFERALQAVA
Sbjct: 241 LIVCNLHLGKPSSRYLSQLKLSHPDHVLVKRAVSAEDNFERALQAVA 287



>Os04g0644700 Coatomer epsilon subunit family protein
          Length = 297

 Score =  406 bits (1043), Expect = e-113,   Method: Compositional matrix adjust.
 Identities = 196/293 (66%), Positives = 245/293 (83%), Gaps = 7/293 (2%)

Query: 2   ASPDLLFNLRNLFYLGAYQAAINNSD-VPG---LDADAAAERDAIVFRSYVALGSYQLVI 57
           ASPD LF LRN FY+GAYQA I     +P    L  DA AERD++++RSY+A+GS+QLVI
Sbjct: 5   ASPDHLFGLRNSFYVGAYQAVITGVQAIPARAALSPDALAERDSLLYRSYIAIGSHQLVI 64

Query: 58  SEIDSSAATSLQAVKLLALYLSGD---KESAIVSLKEWLSDSAVGSNPVLRLIAGIIFMH 114
            EI   AAT LQAV+LL +YLSG    KESAI  L E L+D AVGSNP+LRL+AG + MH
Sbjct: 65  DEIGPGAATPLQAVRLLTVYLSGGAGGKESAIRKLNELLADDAVGSNPILRLVAGTVLMH 124

Query: 115 EQDYTEALKHTHSGGTLDLHALNVQIFIKMHRSDYAEKQLKIMQQIDEDHTLTQLANAWL 174
           E+DY  ALKHT+SGGT++L A+NVQI ++MHRSD+AEKQL+IMQQ+DEDHTLTQLANAW+
Sbjct: 125 ERDYAGALKHTNSGGTMELLAMNVQICLQMHRSDHAEKQLRIMQQLDEDHTLTQLANAWV 184

Query: 175 DIAVGGSKIREAYLIFQDFAEKYPMTGMVLNGKAVCCMHMGSFDEAETLLLEALNKDAKD 234
           D+ +GGSKI+EA+LIFQD +EKYP T ++LNGKA+C MHMG+F++AE LLLE+LNKDAKD
Sbjct: 185 DLVMGGSKIQEAHLIFQDLSEKYPATCLILNGKALCLMHMGNFEDAEGLLLESLNKDAKD 244

Query: 235 PETLANLIVCNLHLGKPSSRYLSQLKLSHPDHVLVKRAVSAEDNFERALQAVA 287
            ETLANL+VC+L+LGK +SRYL+QLKL+HPDH+LVKR  SAED+F+RA QA++
Sbjct: 245 AETLANLVVCSLNLGKSASRYLNQLKLAHPDHMLVKRMSSAEDSFDRACQAIS 297



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.133    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,779,352
Number of extensions: 327429
Number of successful extensions: 784
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 782
Number of HSP's successfully gapped: 2
Length of query: 287
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 187
Effective length of database: 11,814,401
Effective search space: 2209292987
Effective search space used: 2209292987
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 155 (64.3 bits)