BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0593500 Os04g0593500|Os04g0593500
(171 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os04g0593500 Bet v I allergen family protein 345 9e-96
Os04g0593400 Bet v I allergen family protein 165 1e-41
AK110687 164 2e-41
>Os04g0593500 Bet v I allergen family protein
Length = 171
Score = 345 bits (885), Expect = 9e-96, Method: Compositional matrix adjust.
Identities = 171/171 (100%), Positives = 171/171 (100%)
Query: 1 MKGSVCHELETGLPAAEVWEVYGGFLVAQLLPQLVPEVFSKVELVEGDGGVGSVLHVVFA 60
MKGSVCHELETGLPAAEVWEVYGGFLVAQLLPQLVPEVFSKVELVEGDGGVGSVLHVVFA
Sbjct: 1 MKGSVCHELETGLPAAEVWEVYGGFLVAQLLPQLVPEVFSKVELVEGDGGVGSVLHVVFA 60
Query: 61 PGAHTGEFMKEKFIKIDNENYIKEAEVIEGGFLDQGFKKYVVRIEIIGKTENSSVLRSTI 120
PGAHTGEFMKEKFIKIDNENYIKEAEVIEGGFLDQGFKKYVVRIEIIGKTENSSVLRSTI
Sbjct: 61 PGAHTGEFMKEKFIKIDNENYIKEAEVIEGGFLDQGFKKYVVRIEIIGKTENSSVLRSTI 120
Query: 121 KFEAEDASKASSVSTGGLAATAEAVTKYMREQRSSAEPEQVPRQTSDEETF 171
KFEAEDASKASSVSTGGLAATAEAVTKYMREQRSSAEPEQVPRQTSDEETF
Sbjct: 121 KFEAEDASKASSVSTGGLAATAEAVTKYMREQRSSAEPEQVPRQTSDEETF 171
>Os04g0593400 Bet v I allergen family protein
Length = 161
Score = 165 bits (418), Expect = 1e-41, Method: Compositional matrix adjust.
Identities = 79/155 (50%), Positives = 111/155 (71%), Gaps = 2/155 (1%)
Query: 1 MKGSVCHELETGLPAAEVWEVYGGFLVAQLLPQLVPEVFSKVELVEGDGGVGSVLHVVFA 60
M+GS HELET +PA+E+W++YG A+LLP+L+P + +KVELV GDGGVG+++ + F
Sbjct: 1 MRGSQSHELETDVPASELWKIYGTLRAAELLPELLPHILAKVELVTGDGGVGTIVRLTFP 60
Query: 61 PGAHTGEFMKEKFIKIDNENYIKEAEVIEGGFLDQGFKKYVVRIEIIGKTENSSVLRSTI 120
PG + KEKFIK+DN+NY+KEAE +EG L GF Y++R EII K N+SV+RSTI
Sbjct: 61 PGIPGLQSYKEKFIKVDNKNYVKEAEAVEGDILKLGFLSYMIRFEIIRKGANTSVIRSTI 120
Query: 121 KFEAEDASK--ASSVSTGGLAATAEAVTKYMREQR 153
++E D + VST LAATAE +Y+++Q+
Sbjct: 121 EYEIGDEHPELQAMVSTASLAATAEKFAEYIKKQK 155
>AK110687
Length = 161
Score = 164 bits (416), Expect = 2e-41, Method: Compositional matrix adjust.
Identities = 79/155 (50%), Positives = 110/155 (70%), Gaps = 2/155 (1%)
Query: 1 MKGSVCHELETGLPAAEVWEVYGGFLVAQLLPQLVPEVFSKVELVEGDGGVGSVLHVVFA 60
M+GS HELET +PA+E+W++YG A+LLP+L+P + +KVELV GDGGVG+++ + F
Sbjct: 1 MRGSQSHELETDVPASELWKIYGTLRAAELLPELLPHILAKVELVTGDGGVGTIVRLTFP 60
Query: 61 PGAHTGEFMKEKFIKIDNENYIKEAEVIEGGFLDQGFKKYVVRIEIIGKTENSSVLRSTI 120
PG + KEKFIK+DN+NY+KEAE +EG L GF Y++R EII K N+SV+RSTI
Sbjct: 61 PGIPGLQSYKEKFIKVDNKNYVKEAEAVEGDILKLGFLSYMIRFEIIRKGANTSVIRSTI 120
Query: 121 KFEAEDASK--ASSVSTGGLAATAEAVTKYMREQR 153
++E D + VST LAATAE +Y++ Q+
Sbjct: 121 EYEIGDEHPELQAMVSTASLAATAEKFAEYIKTQK 155
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.313 0.131 0.363
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,652,210
Number of extensions: 221290
Number of successful extensions: 513
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 511
Number of HSP's successfully gapped: 3
Length of query: 171
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 77
Effective length of database: 12,127,685
Effective search space: 933831745
Effective search space used: 933831745
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 152 (63.2 bits)