BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0585900 Os04g0585900|AK070920
         (91 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0585900  Protein of unknown function DUF581 family protein   103   2e-23
Os02g0686800  Protein of unknown function DUF581 family protein    68   2e-12

>Os04g0585900 Protein of unknown function DUF581 family protein
          Length = 91

 Score =  103 bits (258), Expect = 2e-23,   Method: Compositional matrix adjust.
 Identities = 53/91 (58%), Positives = 53/91 (58%)

Query: 1  ECSLCGKSLSGDIFMYRGDTPFCSEECRQQQIEVDXXXXXXXXXXXXXXXXXXXEXXXXX 60
          ECSLCGKSLSGDIFMYRGDTPFCSEECRQQQIEVD                   E     
Sbjct: 1  ECSLCGKSLSGDIFMYRGDTPFCSEECRQQQIEVDRAKHRRKKRAAAHAVSVRKEHRNHH 60

Query: 61 XXXXXXXXXXXXXXAIDANPWGTARGPALRV 91
                        AIDANPWGTARGPALRV
Sbjct: 61 HHHRHHHQQPQPRPAIDANPWGTARGPALRV 91



>Os02g0686800 Protein of unknown function DUF581 family protein
          Length = 146

 Score = 67.8 bits (164), Expect = 2e-12,   Method: Compositional matrix adjust.
 Identities = 28/35 (80%), Positives = 33/35 (94%)

Query: 1   ECSLCGKSLSGDIFMYRGDTPFCSEECRQQQIEVD 35
           EC+LC K L+GDIFMYRGDTPFCSEECR++QIE+D
Sbjct: 76  ECTLCRKGLAGDIFMYRGDTPFCSEECRREQIEMD 110



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.137    0.447 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,900,577
Number of extensions: 30350
Number of successful extensions: 86
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 86
Number of HSP's successfully gapped: 2
Length of query: 91
Length of database: 17,035,801
Length adjustment: 61
Effective length of query: 30
Effective length of database: 13,850,747
Effective search space: 415522410
Effective search space used: 415522410
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 149 (62.0 bits)