BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0546400 Os04g0546400|AK059677
         (177 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0546400  Peptidase C12, ubiquitin carboxyl-terminal hyd...   281   2e-76
Os02g0654500  Peptidase C12, ubiquitin carboxyl-terminal hyd...   188   2e-48

>Os04g0546400 Peptidase C12, ubiquitin carboxyl-terminal hydrolase 1 family
           protein
          Length = 177

 Score =  281 bits (718), Expect = 2e-76,   Method: Compositional matrix adjust.
 Identities = 141/177 (79%), Positives = 141/177 (79%)

Query: 1   MLRCGAVYAGIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSEEGIRCFTLLHG 60
           MLRCGAVYAGI                                    SEEGIRCFTLLHG
Sbjct: 1   MLRCGAVYAGIRGSRGGGVLRRLRSRRRDARHGATARARRHLALSSGSEEGIRCFTLLHG 60

Query: 61  REQVEGSYFDRFYKQTADMDPAQRASFLEEDEEMEKAHSVAVSAGDTEAKDGVIEHYVCF 120
           REQVEGSYFDRFYKQTADMDPAQRASFLEEDEEMEKAHSVAVSAGDTEAKDGVIEHYVCF
Sbjct: 61  REQVEGSYFDRFYKQTADMDPAQRASFLEEDEEMEKAHSVAVSAGDTEAKDGVIEHYVCF 120

Query: 121 SCVDDEIFELDGGNSQPISHGPSSPDSLLQDAAKVIKARIAQYPGSLNFNVMALSKQ 177
           SCVDDEIFELDGGNSQPISHGPSSPDSLLQDAAKVIKARIAQYPGSLNFNVMALSKQ
Sbjct: 121 SCVDDEIFELDGGNSQPISHGPSSPDSLLQDAAKVIKARIAQYPGSLNFNVMALSKQ 177



>Os02g0654500 Peptidase C12, ubiquitin carboxyl-terminal hydrolase 1 family
           protein
          Length = 229

 Score =  188 bits (477), Expect = 2e-48,   Method: Compositional matrix adjust.
 Identities = 90/113 (79%), Positives = 98/113 (86%)

Query: 64  VEGSYFDRFYKQTADMDPAQRASFLEEDEEMEKAHSVAVSAGDTEAKDGVIEHYVCFSCV 123
           VEGSYFDRFYKQT DMDP QRA+FLEED+EME AHSVA SAGDT+A   V EH+VCFSCV
Sbjct: 115 VEGSYFDRFYKQTVDMDPVQRAAFLEEDDEMEDAHSVAASAGDTDANVEVNEHFVCFSCV 174

Query: 124 DDEIFELDGGNSQPISHGPSSPDSLLQDAAKVIKARIAQYPGSLNFNVMALSK 176
           D E++ELDG  SQPI HGPSSPD+LLQDAAKVIKARIA  P S+NFNVMALSK
Sbjct: 175 DGELYELDGRKSQPICHGPSSPDTLLQDAAKVIKARIASNPDSMNFNVMALSK 227



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.133    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,075,891
Number of extensions: 194356
Number of successful extensions: 621
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 620
Number of HSP's successfully gapped: 2
Length of query: 177
Length of database: 17,035,801
Length adjustment: 94
Effective length of query: 83
Effective length of database: 12,127,685
Effective search space: 1006597855
Effective search space used: 1006597855
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 152 (63.2 bits)