BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0530400 Os04g0530400|AK067634
         (316 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0530400  t-snare domain containing protein                   564   e-161
Os12g0154400  t-snare domain containing protein                   106   2e-23

>Os04g0530400 t-snare domain containing protein
          Length = 316

 Score =  564 bits (1453), Expect = e-161,   Method: Compositional matrix adjust.
 Identities = 281/316 (88%), Positives = 281/316 (88%)

Query: 1   MSRVRDRTEDFKESVRVAALGHGYTESQLAVLMSSFIIRKPAPKLPFTKAAIKTLESIRE 60
           MSRVRDRTEDFKESVRVAALGHGYTESQLAVLMSSFIIRKPAPKLPFTKAAIKTLESIRE
Sbjct: 1   MSRVRDRTEDFKESVRVAALGHGYTESQLAVLMSSFIIRKPAPKLPFTKAAIKTLESIRE 60

Query: 61  LEKFIVKHRKDYVDLHRTTEQERDNIEHEVSVFVKACKEQIDILKNRIHEEKGGSTKTWL 120
           LEKFIVKHRKDYVDLHRTTEQERDNIEHEVSVFVKACKEQIDILKNRIHEEKGGSTKTWL
Sbjct: 61  LEKFIVKHRKDYVDLHRTTEQERDNIEHEVSVFVKACKEQIDILKNRIHEEKGGSTKTWL 120

Query: 121 GTSDESSRLDLIAHQHGVVLILSERLHSVTVQFDRLRTMRFQDAINRAMPRKRIQKKRET 180
           GTSDESSRLDLIAHQHGVVLILSERLHSVTVQFDRLRTMRFQDAINRAMPRKRIQKKRET
Sbjct: 121 GTSDESSRLDLIAHQHGVVLILSERLHSVTVQFDRLRTMRFQDAINRAMPRKRIQKKRET 180

Query: 181 KAAEPSKPNLVLKSDVSKVEDQEVSTAPLRVQEQLLDDETRALQVELTNLLDAVQETETK 240
           KAAEPSKPNLVLKSDVSKVEDQEVSTAPLRVQEQLLDDETRALQVELTNLLDAVQETETK
Sbjct: 181 KAAEPSKPNLVLKSDVSKVEDQEVSTAPLRVQEQLLDDETRALQVELTNLLDAVQETETK 240

Query: 241 MIEMSALNHLMSTHVXXXXXXXXXXXXXXVEATNNVERGNKELSQAIQRNXXXXXXXXXX 300
           MIEMSALNHLMSTHV              VEATNNVERGNKELSQAIQRN          
Sbjct: 241 MIEMSALNHLMSTHVLQQAQQIQYLYDQAVEATNNVERGNKELSQAIQRNSSSRTFLLLF 300

Query: 301 XXXXXXXXXXXDWYSK 316
                      DWYSK
Sbjct: 301 FFVLTFSVLFLDWYSK 316



>Os12g0154400 t-snare domain containing protein
          Length = 92

 Score =  106 bits (265), Expect = 2e-23,   Method: Compositional matrix adjust.
 Identities = 57/92 (61%), Positives = 57/92 (61%)

Query: 225 VELTNLLDAVQETETKMIEMSALNHLMSTHVXXXXXXXXXXXXXXVEATNNVERGNKELS 284
           VELTNLLDAVQETETKMIEMSALNHLMSTHV              VEATNNVERGNKELS
Sbjct: 1   VELTNLLDAVQETETKMIEMSALNHLMSTHVLQQAQQIQYLYDQAVEATNNVERGNKELS 60

Query: 285 QAIQRNXXXXXXXXXXXXXXXXXXXXXDWYSK 316
           QAIQRN                     DWYSK
Sbjct: 61  QAIQRNSSSRTFLLLFFFVLTFSVLFLDWYSK 92



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.315    0.128    0.347 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 7,929,262
Number of extensions: 277590
Number of successful extensions: 1024
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1024
Number of HSP's successfully gapped: 2
Length of query: 316
Length of database: 17,035,801
Length adjustment: 101
Effective length of query: 215
Effective length of database: 11,762,187
Effective search space: 2528870205
Effective search space used: 2528870205
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 156 (64.7 bits)