BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0496800 Os04g0496800|AK068782
         (753 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0496800  Calcium-binding EF-hand domain containing protein  1337   0.0  
Os02g0608400  EF-Hand type domain containing protein              974   0.0  

>Os04g0496800 Calcium-binding EF-hand domain containing protein
          Length = 753

 Score = 1337 bits (3459), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 669/753 (88%), Positives = 669/753 (88%)

Query: 1   MASRAIIRRRKYALEHTNIPVLLRHSSISTFGQEKFGCEIEQSTASQNSRESNHEKAQYI 60
           MASRAIIRRRKYALEHTNIPVLLRHSSISTFGQEKFGCEIEQSTASQNSRESNHEKAQYI
Sbjct: 1   MASRAIIRRRKYALEHTNIPVLLRHSSISTFGQEKFGCEIEQSTASQNSRESNHEKAQYI 60

Query: 61  LRKQGLLGLSILCHPTRGASLASYESKPQTFGFPLGARYFLQSVRPTSSTAGQPKVGILD 120
           LRKQGLLGLSILCHPTRGASLASYESKPQTFGFPLGARYFLQSVRPTSSTAGQPKVGILD
Sbjct: 61  LRKQGLLGLSILCHPTRGASLASYESKPQTFGFPLGARYFLQSVRPTSSTAGQPKVGILD 120

Query: 121 ERSENQNQNQGKKEASPEECDQAVEGLSTXXXXXXXXLVQEVQKSDQSIIHKFWAILLGI 180
           ERSENQNQNQGKKEASPEECDQAVEGLST        LVQEVQKSDQSIIHKFWAILLGI
Sbjct: 121 ERSENQNQNQGKKEASPEECDQAVEGLSTAKAKAKAKLVQEVQKSDQSIIHKFWAILLGI 180

Query: 181 GPALRAVASMSRADWAAKLKHWKDEFVSTLQHYWLGTKLLWADVRISSRLLVKLAGGKSX 240
           GPALRAVASMSRADWAAKLKHWKDEFVSTLQHYWLGTKLLWADVRISSRLLVKLAGGKS 
Sbjct: 181 GPALRAVASMSRADWAAKLKHWKDEFVSTLQHYWLGTKLLWADVRISSRLLVKLAGGKSL 240

Query: 241 XXXXXXXXXXXXADLFRLVPFAVFIIVPFMEFLLPVFLKMFPNMLPSTFQDKMXXXXXXX 300
                       ADLFRLVPFAVFIIVPFMEFLLPVFLKMFPNMLPSTFQDKM       
Sbjct: 241 TRRERQQLTRTTADLFRLVPFAVFIIVPFMEFLLPVFLKMFPNMLPSTFQDKMKEEEALK 300

Query: 301 XXXXXXMEYARFLQDTAKEMAKEVQTSRSGEMKQTAEDLDEFLNKVRKGGHVSNEEILSF 360
                 MEYARFLQDTAKEMAKEVQTSRSGEMKQTAEDLDEFLNKVRKGGHVSNEEILSF
Sbjct: 301 RKLKARMEYARFLQDTAKEMAKEVQTSRSGEMKQTAEDLDEFLNKVRKGGHVSNEEILSF 360

Query: 361 AKLFNDELTLDNMNRARLVNMCKYMGIQPFGTDHYLTFMLRKKLQEIKNDDKMIQAEGVE 420
           AKLFNDELTLDNMNRARLVNMCKYMGIQPFGTDHYLTFMLRKKLQEIKNDDKMIQAEGVE
Sbjct: 361 AKLFNDELTLDNMNRARLVNMCKYMGIQPFGTDHYLTFMLRKKLQEIKNDDKMIQAEGVE 420

Query: 421 SLSEEELRQACRERGHLGLLSTEEMQNQLRDWLDLSLNHSVPSSLLILSRAFTMSGKMKP 480
           SLSEEELRQACRERGHLGLLSTEEMQNQLRDWLDLSLNHSVPSSLLILSRAFTMSGKMKP
Sbjct: 421 SLSEEELRQACRERGHLGLLSTEEMQNQLRDWLDLSLNHSVPSSLLILSRAFTMSGKMKP 480

Query: 481 EEAVVATLSSLPDEVVDTVGTVLPSEDSVSDRKRKLXXXXXXXXXXXXXXXXXXXXXXXX 540
           EEAVVATLSSLPDEVVDTVGTVLPSEDSVSDRKRKL                        
Sbjct: 481 EEAVVATLSSLPDEVVDTVGTVLPSEDSVSDRKRKLEFLEMQEELIKEEEKRQEKEDKAK 540

Query: 541 LEVPKATEEDVALKEMTEPTAREEKELKKAKVEHDRKEQLCDISQXXXXXXXXXXXXKER 600
           LEVPKATEEDVALKEMTEPTAREEKELKKAKVEHDRKEQLCDISQ            KER
Sbjct: 541 LEVPKATEEDVALKEMTEPTAREEKELKKAKVEHDRKEQLCDISQALAVLASASSVAKER 600

Query: 601 QEFLNLVNKEIELYNTMLEKEGTKGXXXXXXXXXXXXXXSDHAAEIAAGEKVSSALIERV 660
           QEFLNLVNKEIELYNTMLEKEGTKG              SDHAAEIAAGEKVSSALIERV
Sbjct: 601 QEFLNLVNKEIELYNTMLEKEGTKGEEEARRAYKAAREESDHAAEIAAGEKVSSALIERV 660

Query: 661 DAMLQKLEKEIDDVDARIGNRWQLLDSDRDGKVTPDEVAAAANYLKDTIGKEGVQELISN 720
           DAMLQKLEKEIDDVDARIGNRWQLLDSDRDGKVTPDEVAAAANYLKDTIGKEGVQELISN
Sbjct: 661 DAMLQKLEKEIDDVDARIGNRWQLLDSDRDGKVTPDEVAAAANYLKDTIGKEGVQELISN 720

Query: 721 LSKDKDGKILVEDIVKLASQTGESNEQEETPRQ 753
           LSKDKDGKILVEDIVKLASQTGESNEQEETPRQ
Sbjct: 721 LSKDKDGKILVEDIVKLASQTGESNEQEETPRQ 753



>Os02g0608400 EF-Hand type domain containing protein
          Length = 767

 Score =  974 bits (2518), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 537/770 (69%), Positives = 594/770 (77%), Gaps = 20/770 (2%)

Query: 1   MASRAIIRRRKYALEHTNIPVLLRHSSISTFGQEKFGCE----IEQSTASQNSRESNHEK 56
           MASRA+IRRRKY L+H N P L   S  STF   + G E    I Q    Q+S +S  E+
Sbjct: 1   MASRAVIRRRKYLLDHVNAPTL-SLSPFSTFQHGRSGSEDESRIGQRFLEQSSGDSKWEQ 59

Query: 57  AQYILR--KQGLLGLS--ILCHPTRGASLASYESKPQTFGFPLGARYFLQSVRPTSS-TA 111
            QY ++  K  LL L   +L  P  G SL +Y    + FG P+GAR+ LQSVR  S+ TA
Sbjct: 60  GQYGVKLIKGDLLALGNGLLRRPAHGISLPAYGIGRKEFGLPMGARHLLQSVRTASTATA 119

Query: 112 GQPKVGILDERSENQNQNQGKKEASPEECDQAVEGLSTXXXXXXXXLVQEVQKSDQSIIH 171
           GQPK+ I DE+SE+Q QN+ KKEASPEECDQAVEGLS+         VQE  K+ QSI+ 
Sbjct: 120 GQPKLDIEDEQSEDQKQNKRKKEASPEECDQAVEGLSSAKAKAKAKQVQESVKAGQSIVR 179

Query: 172 KFWAILLGIGPALRAVASMSRADWAAKLKHWKDEFVSTLQHYWLGTKLLWADVRISSRLL 231
           KFWA LLGIGPALRAVASMSRADWAAKLKHWKDEFVSTLQHYWLGTKLLWADVRISSRLL
Sbjct: 180 KFWARLLGIGPALRAVASMSRADWAAKLKHWKDEFVSTLQHYWLGTKLLWADVRISSRLL 239

Query: 232 VKLAGGKSXXXXXXXXXXXXXADLFRLVPFAVFIIVPFMEFLLPVFLKMFPNMLPSTFQD 291
           VKLAGGK+             AD+FRLVPFAVFIIVPFMEFLLPVFLK+FPNMLPSTFQD
Sbjct: 240 VKLAGGKNLSRRERQQLTRTTADIFRLVPFAVFIIVPFMEFLLPVFLKLFPNMLPSTFQD 299

Query: 292 KMXXXXXXXXXXXXXMEYARFLQDTAKEMAKEVQTSRSGEMKQTAEDLDEFLNKVRKGGH 351
           KM             MEYA+FLQDTAKEMAKEVQTSRSGE+KQTAEDLDEFLNKVR+G H
Sbjct: 300 KMKEEEALKRKLKARMEYAKFLQDTAKEMAKEVQTSRSGEIKQTAEDLDEFLNKVRRGEH 359

Query: 352 VSNEEILSFAKLFNDELTLDNMNRARLVNMCKYMGIQPFGTDHYLTFMLRKKLQEIKNDD 411
           VSN+EIL+FAKLFNDELTLDNM+R RLVNMCKYMGI+PFGTDHYL FMLRKKLQ+IKNDD
Sbjct: 360 VSNDEILNFAKLFNDELTLDNMSRPRLVNMCKYMGIRPFGTDHYLRFMLRKKLQDIKNDD 419

Query: 412 KMIQAEGVESLSEEELRQACRERGHLGLLSTEEMQNQLRDWLDLSLNHSVPSSLLILSRA 471
           KMIQAEGVESLSEEELRQACRERGHLGLLSTEEM+ QLRDWLDLSLNH+VPSSLLILSRA
Sbjct: 420 KMIQAEGVESLSEEELRQACRERGHLGLLSTEEMRQQLRDWLDLSLNHAVPSSLLILSRA 479

Query: 472 FTMSGKMKPEEAVVATLSSLPDEVVDTVGTVLPSEDSVSDRKRKL--------XXXXXXX 523
           FT+SGKMKPEEAVVATLSSLPDEVVDTVGTVLPSEDSVS+R+RKL               
Sbjct: 480 FTVSGKMKPEEAVVATLSSLPDEVVDTVGTVLPSEDSVSERRRKLEFLEMQEELIKEEEK 539

Query: 524 XXXXXXXXXXXXXXXXXLEVPKATEEDVALKEMTEPTAREEKELKKAKVEHDRKEQLCDI 583
                            L+ PKA EED+ALKEMT PTAREE+EL++AK +HD KE+LC+ 
Sbjct: 540 KKEKEEKAKQEKEEKAKLKEPKAAEEDLALKEMTGPTAREEEELREAK-QHD-KEKLCNF 597

Query: 584 SQXXXXXXXXXXXXKERQEFLNLVNKEIELYNTMLEKEGTKGXXXXXXXXXXXXXXSDHA 643
           S+            KERQEFL+LVNKEIELYN+MLEKEGT+G              SD A
Sbjct: 598 SRALAVLASASSVSKERQEFLSLVNKEIELYNSMLEKEGTEGEEEAKKAYMAAREESDKA 657

Query: 644 AEIAAGEKVSSALIERVDAMLQKLEKEIDDVDARIGNRWQLLDSDRDGKVTPDEVAAAAN 703
           AE+   EKVSSALIE+VDAMLQKLEKEIDDVDA+IGNRWQ+LD D DGKVTP+EVA+AA 
Sbjct: 658 AEVDEEEKVSSALIEKVDAMLQKLEKEIDDVDAQIGNRWQILDRDLDGKVTPEEVASAAA 717

Query: 704 YLKDTIGKEGVQELISNLSKDKDGKILVEDIVKLASQTGESNEQEETPRQ 753
           YLKDTIGKEGVQEL+SNLSKDKDGKI VEDIVKLASQT E+NE EE  RQ
Sbjct: 718 YLKDTIGKEGVQELVSNLSKDKDGKIRVEDIVKLASQTDENNEDEEEGRQ 767



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.314    0.130    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 20,869,927
Number of extensions: 761359
Number of successful extensions: 2239
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 2233
Number of HSP's successfully gapped: 2
Length of query: 753
Length of database: 17,035,801
Length adjustment: 108
Effective length of query: 645
Effective length of database: 11,396,689
Effective search space: 7350864405
Effective search space used: 7350864405
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 160 (66.2 bits)