BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0463400 Os04g0463400|AK059730
         (252 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0463400  Protein of unknown function DUF125, transmembr...   412   e-115
Os09g0396900  Protein of unknown function DUF125, transmembr...   191   5e-49

>Os04g0463400 Protein of unknown function DUF125, transmembrane family protein
          Length = 252

 Score =  412 bits (1058), Expect = e-115,   Method: Compositional matrix adjust.
 Identities = 209/252 (82%), Positives = 209/252 (82%)

Query: 1   MAAATDGGGLPLLADKXXXXXXXXXPERHFTSGEVVRDVIMGVSDGLTVPFXXXXXXXXX 60
           MAAATDGGGLPLLADK         PERHFTSGEVVRDVIMGVSDGLTVPF         
Sbjct: 1   MAAATDGGGLPLLADKAASHSHHHHPERHFTSGEVVRDVIMGVSDGLTVPFALAAGLSGA 60

Query: 61  XXXXXXXXTAGLAEVAAGAISMGLGGYLAAKSEADHYQREMKREQEEIIAVPDTEAAEIG 120
                   TAGLAEVAAGAISMGLGGYLAAKSEADHYQREMKREQEEIIAVPDTEAAEIG
Sbjct: 61  SAPSSLVLTAGLAEVAAGAISMGLGGYLAAKSEADHYQREMKREQEEIIAVPDTEAAEIG 120

Query: 121 EIMSQYGLEPHEYGPVVDGLRRNPQAWLDFMMRFELGLEKPDPKRAIQSALTIALSYVIG 180
           EIMSQYGLEPHEYGPVVDGLRRNPQAWLDFMMRFELGLEKPDPKRAIQSALTIALSYVIG
Sbjct: 121 EIMSQYGLEPHEYGPVVDGLRRNPQAWLDFMMRFELGLEKPDPKRAIQSALTIALSYVIG 180

Query: 181 GLVPLLPYMFISTAQNAMLTSVGVTLVALLFFGYIKGRFTGNRPFLSAVQTXXXXXXXXX 240
           GLVPLLPYMFISTAQNAMLTSVGVTLVALLFFGYIKGRFTGNRPFLSAVQT         
Sbjct: 181 GLVPLLPYMFISTAQNAMLTSVGVTLVALLFFGYIKGRFTGNRPFLSAVQTAIIGALASA 240

Query: 241 XXXXXXXXVQTR 252
                   VQTR
Sbjct: 241 AAYGMAKAVQTR 252



>Os09g0396900 Protein of unknown function DUF125, transmembrane family protein
          Length = 166

 Score =  191 bits (485), Expect = 5e-49,   Method: Compositional matrix adjust.
 Identities = 92/137 (67%), Positives = 108/137 (78%)

Query: 95  DHYQREMKREQEEIIAVPDTEAAEIGEIMSQYGLEPHEYGPVVDGLRRNPQAWLDFMMRF 154
           +  QR +  E  E        AAEI +I+SQYGL P EYGPVV+ LR NP+AWL+FMM+F
Sbjct: 9   EEKQRLLLDEHTEKHFTAGEVAAEIADILSQYGLGPEEYGPVVNSLRSNPKAWLEFMMKF 68

Query: 155 ELGLEKPDPKRAIQSALTIALSYVIGGLVPLLPYMFISTAQNAMLTSVGVTLVALLFFGY 214
           ELGLEKP+P+RA+ SA TIAL+YV+GGLVPLLPYMF+ TA  AM TSV VTL ALLFFGY
Sbjct: 69  ELGLEKPEPRRALMSAGTIALAYVVGGLVPLLPYMFVPTADRAMATSVVVTLAALLFFGY 128

Query: 215 IKGRFTGNRPFLSAVQT 231
           +KGRFTGNRPF+SA QT
Sbjct: 129 VKGRFTGNRPFISAFQT 145



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.137    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,755,770
Number of extensions: 239054
Number of successful extensions: 537
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 536
Number of HSP's successfully gapped: 2
Length of query: 252
Length of database: 17,035,801
Length adjustment: 98
Effective length of query: 154
Effective length of database: 11,918,829
Effective search space: 1835499666
Effective search space used: 1835499666
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 155 (64.3 bits)