BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0404800 Os04g0404800|J100029A19
         (139 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0404800  Conserved hypothetical protein                      280   2e-76
Os02g0525900  Similar to Acetyl-coenzyme A synthetase 2 (EC ...   181   1e-46

>Os04g0404800 Conserved hypothetical protein
          Length = 139

 Score =  280 bits (717), Expect = 2e-76,   Method: Compositional matrix adjust.
 Identities = 139/139 (100%), Positives = 139/139 (100%)

Query: 1   MLMELPIAMLACARIGAVHSVIITPLPGAWPLKPGSATFPFFGVQVKGQGIYAFVTLVDG 60
           MLMELPIAMLACARIGAVHSVIITPLPGAWPLKPGSATFPFFGVQVKGQGIYAFVTLVDG
Sbjct: 1   MLMELPIAMLACARIGAVHSVIITPLPGAWPLKPGSATFPFFGVQVKGQGIYAFVTLVDG 60

Query: 61  VPYSDELRKSLVMTVRSQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASRQLDELGD 120
           VPYSDELRKSLVMTVRSQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASRQLDELGD
Sbjct: 61  VPYSDELRKSLVMTVRSQIGAFAAPDKIHWAPGLPKTRSGKIMRRILRKIASRQLDELGD 120

Query: 121 TSTLAEPGVVDQLIALADS 139
           TSTLAEPGVVDQLIALADS
Sbjct: 121 TSTLAEPGVVDQLIALADS 139



>Os02g0525900 Similar to Acetyl-coenzyme A synthetase 2 (EC 6.2.1.1)
           (Acetate--CoA ligase 2) (Acyl-activating enzyme 2)
          Length = 705

 Score =  181 bits (460), Expect = 1e-46,   Method: Composition-based stats.
 Identities = 86/94 (91%), Positives = 93/94 (98%)

Query: 45  QVKGQGIYAFVTLVDGVPYSDELRKSLVMTVRSQIGAFAAPDKIHWAPGLPKTRSGKIMR 104
           +VKGQGIYAFVTLVDGVPYS+ELRKSL++TVR+QIGAFAAPDKIHWAPGLPKTRSGKIMR
Sbjct: 611 EVKGQGIYAFVTLVDGVPYSEELRKSLILTVRNQIGAFAAPDKIHWAPGLPKTRSGKIMR 670

Query: 105 RILRKIASRQLDELGDTSTLAEPGVVDQLIALAD 138
           RILRKIAS+QLDELGDTSTLA+PGVVDQLIAL D
Sbjct: 671 RILRKIASKQLDELGDTSTLADPGVVDQLIALKD 704



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.323    0.139    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 4,524,232
Number of extensions: 173041
Number of successful extensions: 345
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 345
Number of HSP's successfully gapped: 2
Length of query: 139
Length of database: 17,035,801
Length adjustment: 90
Effective length of query: 49
Effective length of database: 12,336,541
Effective search space: 604490509
Effective search space used: 604490509
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 151 (62.8 bits)