BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0398300 Os04g0398300|AK061199
         (292 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0398300  Similar to Glycyl-tRNA synthetase, mitochondri...   579   e-165
Os08g0538000  Similar to Glycyl-tRNA synthetase, mitochondri...   466   e-132
AK059027                                                           98   6e-21

>Os04g0398300 Similar to Glycyl-tRNA synthetase, mitochondrial precursor (EC
           6.1.1.14) (Glycine--tRNA ligase) (GlyRS)
          Length = 292

 Score =  579 bits (1492), Expect = e-165,   Method: Compositional matrix adjust.
 Identities = 280/280 (100%), Positives = 280/280 (100%)

Query: 1   AEIECSFGWIECVGIADRSAYDLRAHSDKSGVALEAHEKFAEPREVEKLVITPSKKELGL 60
           AEIECSFGWIECVGIADRSAYDLRAHSDKSGVALEAHEKFAEPREVEKLVITPSKKELGL
Sbjct: 1   AEIECSFGWIECVGIADRSAYDLRAHSDKSGVALEAHEKFAEPREVEKLVITPSKKELGL 60

Query: 61  AFKGNQRMVLEALEAMSETEALNMKSALESKGEVEFKVCTLGKDVTIKKSMVSINMEKKK 120
           AFKGNQRMVLEALEAMSETEALNMKSALESKGEVEFKVCTLGKDVTIKKSMVSINMEKKK
Sbjct: 61  AFKGNQRMVLEALEAMSETEALNMKSALESKGEVEFKVCTLGKDVTIKKSMVSINMEKKK 120

Query: 121 EHQRKFTPSVIEPSFGIGRIIYCLFEHCFYQRPGKAEDEQLNVFGFPPLVAPIKCTVFPL 180
           EHQRKFTPSVIEPSFGIGRIIYCLFEHCFYQRPGKAEDEQLNVFGFPPLVAPIKCTVFPL
Sbjct: 121 EHQRKFTPSVIEPSFGIGRIIYCLFEHCFYQRPGKAEDEQLNVFGFPPLVAPIKCTVFPL 180

Query: 181 VKIEKFEVVAKKISKALTAAGISHIIDMTGNTIGKRYARTDEIGVPLAITVDNTTSVTVR 240
           VKIEKFEVVAKKISKALTAAGISHIIDMTGNTIGKRYARTDEIGVPLAITVDNTTSVTVR
Sbjct: 181 VKIEKFEVVAKKISKALTAAGISHIIDMTGNTIGKRYARTDEIGVPLAITVDNTTSVTVR 240

Query: 241 DRDSKDQIRVEVDEVASVVKEVTDGQSTWADIMWRYPAHT 280
           DRDSKDQIRVEVDEVASVVKEVTDGQSTWADIMWRYPAHT
Sbjct: 241 DRDSKDQIRVEVDEVASVVKEVTDGQSTWADIMWRYPAHT 280



>Os08g0538000 Similar to Glycyl-tRNA synthetase, mitochondrial precursor (EC
           6.1.1.14) (Glycine--tRNA ligase) (GlyRS)
          Length = 698

 Score =  466 bits (1200), Expect = e-132,   Method: Compositional matrix adjust.
 Identities = 220/279 (78%), Positives = 251/279 (89%)

Query: 1   AEIECSFGWIECVGIADRSAYDLRAHSDKSGVALEAHEKFAEPREVEKLVITPSKKELGL 60
           AEIECS+GWIECVGIADRSAYDLRAHSDKSGV L AHEKF++PREVEKLVI PSKK+LGL
Sbjct: 412 AEIECSYGWIECVGIADRSAYDLRAHSDKSGVPLVAHEKFSKPREVEKLVIVPSKKDLGL 471

Query: 61  AFKGNQRMVLEALEAMSETEALNMKSALESKGEVEFKVCTLGKDVTIKKSMVSINMEKKK 120
           AFKGNQ+MV+EALEAMSE EA++MK+ALESKGE  F+VCTLGKDV I K MVSI+MEKK 
Sbjct: 472 AFKGNQKMVVEALEAMSEKEAMDMKAALESKGETNFQVCTLGKDVVITKKMVSISMEKKL 531

Query: 121 EHQRKFTPSVIEPSFGIGRIIYCLFEHCFYQRPGKAEDEQLNVFGFPPLVAPIKCTVFPL 180
           EHQR FTPSVIEPSFGIGRIIYCLFEH FY RP K+E+EQLNVF FPP+VAPIKCTVFPL
Sbjct: 532 EHQRVFTPSVIEPSFGIGRIIYCLFEHSFYTRPSKSEEEQLNVFRFPPIVAPIKCTVFPL 591

Query: 181 VKIEKFEVVAKKISKALTAAGISHIIDMTGNTIGKRYARTDEIGVPLAITVDNTTSVTVR 240
           VK ++F+  AK I KALT AGISHIID T  +IG+RYARTDEIGVP A+TVD+ TSVT+R
Sbjct: 592 VKNQEFDDAAKVIDKALTTAGISHIIDTTAISIGRRYARTDEIGVPFAVTVDSATSVTIR 651

Query: 241 DRDSKDQIRVEVDEVASVVKEVTDGQSTWADIMWRYPAH 279
           +RDSK+QIRV +DEVASVVK++TDGQSTWAD+ ++YP+H
Sbjct: 652 ERDSKEQIRVGIDEVASVVKQLTDGQSTWADVSFKYPSH 690



>AK059027 
          Length = 179

 Score = 98.2 bits (243), Expect = 6e-21,   Method: Compositional matrix adjust.
 Identities = 45/45 (100%), Positives = 45/45 (100%)

Query: 1   AEIECSFGWIECVGIADRSAYDLRAHSDKSGVALEAHEKFAEPRE 45
           AEIECSFGWIECVGIADRSAYDLRAHSDKSGVALEAHEKFAEPRE
Sbjct: 106 AEIECSFGWIECVGIADRSAYDLRAHSDKSGVALEAHEKFAEPRE 150



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.317    0.133    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,876,866
Number of extensions: 337459
Number of successful extensions: 862
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 862
Number of HSP's successfully gapped: 3
Length of query: 292
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 192
Effective length of database: 11,814,401
Effective search space: 2268364992
Effective search space used: 2268364992
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 155 (64.3 bits)