BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0378200 Os04g0378200|AK103076
         (215 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0378200  Sterile alpha motif SAM domain containing protein   362   e-100
Os08g0526500  Similar to SOX-1 protein                            103   1e-22
Os04g0465000  Sterile alpha motif homology domain containing...    75   3e-14

>Os04g0378200 Sterile alpha motif SAM domain containing protein
          Length = 215

 Score =  362 bits (928), Expect = e-100,   Method: Compositional matrix adjust.
 Identities = 183/215 (85%), Positives = 183/215 (85%)

Query: 1   MYADQISTGRKRSIHDRIDGDQPXXXXXXXXXXXXXXNPPSKRQRQTDEKWKHDLYREDD 60
           MYADQISTGRKRSIHDRIDGDQP              NPPSKRQRQTDEKWKHDLYREDD
Sbjct: 1   MYADQISTGRKRSIHDRIDGDQPAARAGAGAGGRGARNPPSKRQRQTDEKWKHDLYREDD 60

Query: 61  EPASKSIDPRDLRLKLQKKSSQQSFAGQRGSGVRDLREMLSGTMHPQPVNADPPKAKPSS 120
           EPASKSIDPRDLRLKLQKKSSQQSFAGQRGSGVRDLREMLSGTMHPQPVNADPPKAKPSS
Sbjct: 61  EPASKSIDPRDLRLKLQKKSSQQSFAGQRGSGVRDLREMLSGTMHPQPVNADPPKAKPSS 120

Query: 121 EIVKVTRRENADVMPVRXXXXXXXXXXXXXXXXXXADSPLDIFLKSLGLEKYSITFQAEE 180
           EIVKVTRRENADVMPVR                  ADSPLDIFLKSLGLEKYSITFQAEE
Sbjct: 121 EIVKVTRRENADVMPVRQSKKVPKPTSSKKTSQPKADSPLDIFLKSLGLEKYSITFQAEE 180

Query: 181 VDMAALRHMTDSDLKALGIPMGPRKKIMLALESRA 215
           VDMAALRHMTDSDLKALGIPMGPRKKIMLALESRA
Sbjct: 181 VDMAALRHMTDSDLKALGIPMGPRKKIMLALESRA 215



>Os08g0526500 Similar to SOX-1 protein
          Length = 237

 Score =  103 bits (256), Expect = 1e-22,   Method: Compositional matrix adjust.
 Identities = 87/221 (39%), Positives = 104/221 (47%), Gaps = 16/221 (7%)

Query: 1   MYADQISTGRKRSIHDRIDGDQPXXXXXXXXXXXXXXN----PPS-KRQRQTDEKWKHDL 55
           MY+D +S GRKRS+ DR+                        P S KR RQ D  W+ +L
Sbjct: 1   MYSDSVS-GRKRSVMDRLGSGGGGGGGGGGGGGGGGGGSRPRPDSVKRFRQADGTWRREL 59

Query: 56  YREDDEPASKSIDPRDLRLKLQKKSSQQSFAGQRGSGVRDLREMLSGTMHPQPVNADP-- 113
           Y +     S S  P       QK   QQ+    R S V DLRE LSG   PQ +N+    
Sbjct: 60  YMDSVGVQSSSGVPSRNIQGNQKPHLQQNTQVMRKSSVPDLREKLSGVQRPQ-LNSTVQI 118

Query: 114 PKAKP--SSEIVKVTRRENAD-----VMPVRXXXXXXXXXXXXXXXXXXADSPLDIFLKS 166
           PK+ P  S+    V +RE        V                       DS LD  LKS
Sbjct: 119 PKSVPEISTSAKPVQKREPVQKREPPVNAALPATKKVNEPSAPKQCQEKTDSSLDRLLKS 178

Query: 167 LGLEKYSITFQAEEVDMAALRHMTDSDLKALGIPMGPRKKI 207
           L +EKYSI FQAEEVDM AL HM + D+K+LGIPMGPRKKI
Sbjct: 179 LDVEKYSINFQAEEVDMKALLHMNEEDMKSLGIPMGPRKKI 219



>Os04g0465000 Sterile alpha motif homology domain containing protein
          Length = 346

 Score = 75.1 bits (183), Expect = 3e-14,   Method: Compositional matrix adjust.
 Identities = 36/55 (65%), Positives = 41/55 (74%), Gaps = 2/55 (3%)

Query: 159 PLDI--FLKSLGLEKYSITFQAEEVDMAALRHMTDSDLKALGIPMGPRKKIMLAL 211
           PL +   L SLGLEKY   F AEEVDMAAL  M DSDLK +G+PMGPRKKI+ A+
Sbjct: 284 PLTVTGLLNSLGLEKYVFLFHAEEVDMAALSQMGDSDLKEIGVPMGPRKKILQAV 338



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.314    0.131    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 6,502,565
Number of extensions: 239395
Number of successful extensions: 468
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 465
Number of HSP's successfully gapped: 3
Length of query: 215
Length of database: 17,035,801
Length adjustment: 97
Effective length of query: 118
Effective length of database: 11,971,043
Effective search space: 1412583074
Effective search space used: 1412583074
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 154 (63.9 bits)