BLASTP 2.2.23 [Feb-03-2010]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Os04g0350100 Os04g0350100|AK102139
(151 letters)
Database: rap3
52,214 sequences; 17,035,801 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Os04g0350100 Proteinase inhibitor I25, cystatin domain cont... 178 1e-45
Os01g0915200 Similar to Cystatin 74 5e-14
Os01g0915401 Proteinase inhibitor I25, cystatin domain cont... 65 1e-11
>Os04g0350100 Proteinase inhibitor I25, cystatin domain containing protein
Length = 151
Score = 178 bits (452), Expect = 1e-45, Method: Compositional matrix adjust.
Identities = 98/151 (64%), Positives = 98/151 (64%)
Query: 1 MATSPMXXXXXXXXXXXXXXTGDEXXXXXXXXXXXXVLVGGRTEIRDVGSNKAVQSLGRF 60
MATSPM TGDE VLVGGRTEIRDVGSNKAVQSLGRF
Sbjct: 1 MATSPMLFLVSLLLVLVAAATGDEASPSNAAAPAAPVLVGGRTEIRDVGSNKAVQSLGRF 60
Query: 61 AVAEHNRRLRHGGSGGPADPVPVKLAFARVVEAQKQVVSGVAYYLKVXXXXXXXXXXXXX 120
AVAEHNRRLRHGGSGGPADPVPVKLAFARVVEAQKQVVSGVAYYLKV
Sbjct: 61 AVAEHNRRLRHGGSGGPADPVPVKLAFARVVEAQKQVVSGVAYYLKVAASARDPRGGAAA 120
Query: 121 XXXXXXXXXXXXXXWLKSKELVSFTPASSTK 151
WLKSKELVSFTPASSTK
Sbjct: 121 GGDRVFDAVVVVKAWLKSKELVSFTPASSTK 151
>Os01g0915200 Similar to Cystatin
Length = 186
Score = 73.6 bits (179), Expect = 5e-14, Method: Compositional matrix adjust.
Identities = 43/108 (39%), Positives = 57/108 (52%), Gaps = 7/108 (6%)
Query: 43 TEIRDVGSNKAVQSLGRFAVAEHNRRLRHGGSGGPADPVPVKLAFARVVEAQKQVVSGVA 102
TE+RDV ++ VQ LGRF+V EHNRR R G V+L F RVV AQ+QVVSG+
Sbjct: 82 TEVRDVEGDREVQELGRFSVEEHNRRRRSRDCGD------VRLEFGRVVAAQRQVVSGLK 135
Query: 103 YYLKVXXXXXXXXXXXXXXXXXXXXXXXXXXXWLKSKELVSFTPASST 150
YYL+V WL+S+ L++F PA+ +
Sbjct: 136 YYLRV-AAAEEGAAGQNGGEPRVFDAVVVVKPWLESRTLLTFAPAADS 182
>Os01g0915401 Proteinase inhibitor I25, cystatin domain containing protein
Length = 148
Score = 65.5 bits (158), Expect = 1e-11, Method: Compositional matrix adjust.
Identities = 41/106 (38%), Positives = 52/106 (49%), Gaps = 15/106 (14%)
Query: 43 TEIRDVGSNKAVQSLGRFAVAEHNRRLRHGGSGGPADPVPVKLAFARVVEAQKQVVSGVA 102
T++ DV N+ VQ LG F V EHNRR GGS L F+RVV AQ QVVSG+
Sbjct: 53 TDVEDVEGNREVQELGLFCVVEHNRR---GGSATRGR----GLVFSRVVAAQTQVVSGIK 105
Query: 103 YYLKVXXXXXXXXXXXXXXXXXXXXXXXXXXXWLKSKELVSFTPAS 148
YYL++ W+ S+E+VSF PA+
Sbjct: 106 YYLRI--------AAQEADDELVFDAVVVVKAWVPSREMVSFVPAA 143
Database: rap3
Posted date: Nov 19, 2010 6:03 PM
Number of letters in database: 17,035,801
Number of sequences in database: 52,214
Lambda K H
0.315 0.130 0.370
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,351,942
Number of extensions: 111542
Number of successful extensions: 436
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 431
Number of HSP's successfully gapped: 3
Length of query: 151
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 59
Effective length of database: 12,232,113
Effective search space: 721694667
Effective search space used: 721694667
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 151 (62.8 bits)