BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0308500 Os04g0308500|AK108905
         (210 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0308500  Conserved hypothetical protein                      437   e-123
Os04g0310000  Conserved hypothetical protein                      407   e-114
Os04g0310100                                                      135   2e-32
Os04g0308401  Conserved hypothetical protein                      135   2e-32

>Os04g0308500 Conserved hypothetical protein
          Length = 210

 Score =  437 bits (1125), Expect = e-123,   Method: Compositional matrix adjust.
 Identities = 210/210 (100%), Positives = 210/210 (100%)

Query: 1   MANCFGDVVDNYKLDEMERYVGKAKRQEDRAREAMNLVNEDGKDKKAASYVQGVKDWYGN 60
           MANCFGDVVDNYKLDEMERYVGKAKRQEDRAREAMNLVNEDGKDKKAASYVQGVKDWYGN
Sbjct: 1   MANCFGDVVDNYKLDEMERYVGKAKRQEDRAREAMNLVNEDGKDKKAASYVQGVKDWYGN 60

Query: 61  GESTLCLVYNATGATLRHVADHDWWGFIGRTPYPTEIGNGQWAAFHHVHKSGDSSGSEAA 120
           GESTLCLVYNATGATLRHVADHDWWGFIGRTPYPTEIGNGQWAAFHHVHKSGDSSGSEAA
Sbjct: 61  GESTLCLVYNATGATLRHVADHDWWGFIGRTPYPTEIGNGQWAAFHHVHKSGDSSGSEAA 120

Query: 121 VVYRGTNADGVERDLLVAWSTPWSSFYRNKAYCAVGGVDSFQGDWEQLYDKVNNAAYTCN 180
           VVYRGTNADGVERDLLVAWSTPWSSFYRNKAYCAVGGVDSFQGDWEQLYDKVNNAAYTCN
Sbjct: 121 VVYRGTNADGVERDLLVAWSTPWSSFYRNKAYCAVGGVDSFQGDWEQLYDKVNNAAYTCN 180

Query: 181 ADSDGFKIDASTATGDSPVFTATIKIHFSQ 210
           ADSDGFKIDASTATGDSPVFTATIKIHFSQ
Sbjct: 181 ADSDGFKIDASTATGDSPVFTATIKIHFSQ 210



>Os04g0310000 Conserved hypothetical protein
          Length = 246

 Score =  407 bits (1046), Expect = e-114,   Method: Compositional matrix adjust.
 Identities = 195/196 (99%), Positives = 196/196 (100%)

Query: 1   MANCFGDVVDNYKLDEMERYVGKAKRQEDRAREAMNLVNEDGKDKKAASYVQGVKDWYGN 60
           MANCFGDVVDNYKLDEMERYVGKAKRQEDRAREAMNLVNEDGKDKKAASYVQGVKDWYGN
Sbjct: 1   MANCFGDVVDNYKLDEMERYVGKAKRQEDRAREAMNLVNEDGKDKKAASYVQGVKDWYGN 60

Query: 61  GESTLCLVYNATGATLRHVADHDWWGFIGRTPYPTEIGNGQWAAFHHVHKSGDSSGSEAA 120
           GESTLCLVYNATGATLRHVADHDWWGFIGRTPYPTEIGNGQWAAFHHVHKSGDSSGSEAA
Sbjct: 61  GESTLCLVYNATGATLRHVADHDWWGFIGRTPYPTEIGNGQWAAFHHVHKSGDSSGSEAA 120

Query: 121 VVYRGTNADGVERDLLVAWSTPWSSFYRNKAYCAVGGVDSFQGDWEQLYDKVNNAAYTCN 180
           VVYRGTNADGVERDLLVAWSTPWSSFYRNKAYCAVGGVDSFQGDWEQLYDKVNNAAYTCN
Sbjct: 121 VVYRGTNADGVERDLLVAWSTPWSSFYRNKAYCAVGGVDSFQGDWEQLYDKVNNAAYTCN 180

Query: 181 ADSDGFKIDASTATGD 196
           ADSDGFKIDASTATG+
Sbjct: 181 ADSDGFKIDASTATGE 196



>Os04g0310100 
          Length = 203

 Score =  135 bits (340), Expect = 2e-32,   Method: Compositional matrix adjust.
 Identities = 75/173 (43%), Positives = 97/173 (56%), Gaps = 6/173 (3%)

Query: 17  MERYVGKAKRQEDRAREAMNLVNEDGKDKKAASYVQGVKDWYGNGESTLCLVYNATGATL 76
           M RY GK+  Q DRAREAM L++ +GK+  A  +  G+K  YG+G S + LVYNATGA L
Sbjct: 1   MSRYAGKSISQVDRAREAMRLIHAEGKNLDALQHALGLKASYGDGVSAMVLVYNATGAAL 60

Query: 77  RHV----ADHDWWGFIGRTPYPTEIGNGQWAAFHHVHKSGDSSGSEAAVVYRGTNADGVE 132
             V       DW+G++     P    NGQW AF H H +  S G EAA V+RG + DG  
Sbjct: 61  ELVDGEGGSMDWYGYVYHEQPPASFQNGQWLAFLHAHPTAQSIGCEAARVFRGRDVDGQV 120

Query: 133 RDLLVAWSTPWSSFYRNKAYCAVGGVDSFQGDWEQLY-DKVNNAAYTCNADSD 184
           RD +VAWS PWS+  +N AY  V   D F   W  +  +K+  A   C   +D
Sbjct: 121 RDFMVAWSLPWSA-TQNSAYTEVREKDHFPNYWGYIKEEKLEKAGKICTDQTD 172



>Os04g0308401 Conserved hypothetical protein
          Length = 203

 Score =  135 bits (340), Expect = 2e-32,   Method: Compositional matrix adjust.
 Identities = 75/173 (43%), Positives = 97/173 (56%), Gaps = 6/173 (3%)

Query: 17  MERYVGKAKRQEDRAREAMNLVNEDGKDKKAASYVQGVKDWYGNGESTLCLVYNATGATL 76
           M RY GK+  Q DRAREAM L++ +GK+  A  +  G+K  YG+G S + LVYNATGA L
Sbjct: 1   MSRYAGKSISQVDRAREAMRLIHAEGKNLDALQHALGLKASYGDGVSAMVLVYNATGAAL 60

Query: 77  RHV----ADHDWWGFIGRTPYPTEIGNGQWAAFHHVHKSGDSSGSEAAVVYRGTNADGVE 132
             V       DW+G++     P    NGQW AF H H +  S G EAA V+RG + DG  
Sbjct: 61  ELVDGEGGSMDWYGYVYHEQPPASFQNGQWLAFLHAHPTAQSIGCEAARVFRGRDVDGQV 120

Query: 133 RDLLVAWSTPWSSFYRNKAYCAVGGVDSFQGDWEQLY-DKVNNAAYTCNADSD 184
           RD +VAWS PWS+  +N AY  V   D F   W  +  +K+  A   C   +D
Sbjct: 121 RDFMVAWSLPWSA-TQNSAYTEVREKDHFPNYWGYIKEEKLEKAGKICTDQTD 172



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.316    0.132    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,100,827
Number of extensions: 373133
Number of successful extensions: 866
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 864
Number of HSP's successfully gapped: 4
Length of query: 210
Length of database: 17,035,801
Length adjustment: 96
Effective length of query: 114
Effective length of database: 12,023,257
Effective search space: 1370651298
Effective search space used: 1370651298
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 154 (63.9 bits)