BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0271000 Os04g0271000|AK069000
         (483 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0271000  Similar to NAD-dependent deacetylase sirtuin-7...  1001   0.0  
Os12g0179800  Silent information regulator protein Sir2 fami...   101   1e-21

>Os04g0271000 Similar to NAD-dependent deacetylase sirtuin-7 (EC 3.5.1.-)
           (SIR2-like protein 7). Splice isoform 2
          Length = 483

 Score = 1001 bits (2587), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 483/483 (100%), Positives = 483/483 (100%)

Query: 1   MSLGYAEKLSYREDVGNVGMPEIFDSPELLHKKIEELAVMVRESKHLVVFTGAGISTSSG 60
           MSLGYAEKLSYREDVGNVGMPEIFDSPELLHKKIEELAVMVRESKHLVVFTGAGISTSSG
Sbjct: 1   MSLGYAEKLSYREDVGNVGMPEIFDSPELLHKKIEELAVMVRESKHLVVFTGAGISTSSG 60

Query: 61  IPDFRGPKGVWTLQRSGKGVPGASLPFHRAVPTLTHMALVELEKTGRLKFVISQNVDSLH 120
           IPDFRGPKGVWTLQRSGKGVPGASLPFHRAVPTLTHMALVELEKTGRLKFVISQNVDSLH
Sbjct: 61  IPDFRGPKGVWTLQRSGKGVPGASLPFHRAVPTLTHMALVELEKTGRLKFVISQNVDSLH 120

Query: 121 LRSGLPREKLAELHGNSFKEICPSCKKEYLRDFEIETIGLKDTPRRCSDKNCGARLKDTV 180
           LRSGLPREKLAELHGNSFKEICPSCKKEYLRDFEIETIGLKDTPRRCSDKNCGARLKDTV
Sbjct: 121 LRSGLPREKLAELHGNSFKEICPSCKKEYLRDFEIETIGLKDTPRRCSDKNCGARLKDTV 180

Query: 181 LDWEDALPPEEMDAAKEQCQKADLVLCLGTSLQITPACNMPLLSLKNGGRVAIVNLQATP 240
           LDWEDALPPEEMDAAKEQCQKADLVLCLGTSLQITPACNMPLLSLKNGGRVAIVNLQATP
Sbjct: 181 LDWEDALPPEEMDAAKEQCQKADLVLCLGTSLQITPACNMPLLSLKNGGRVAIVNLQATP 240

Query: 241 KDKKASLVIHGLVDKVIAGVMYMMNLRIPPYIRTDFVQISLRNSVKKKCVRWTLRVTSIH 300
           KDKKASLVIHGLVDKVIAGVMYMMNLRIPPYIRTDFVQISLRNSVKKKCVRWTLRVTSIH
Sbjct: 241 KDKKASLVIHGLVDKVIAGVMYMMNLRIPPYIRTDFVQISLRNSVKKKCVRWTLRVTSIH 300

Query: 301 GLRAPLPFLRSVEVSFPERPDMKPVVLKEQPFSLQRETSMNRPFVMLLTFNFSDGCGCSS 360
           GLRAPLPFLRSVEVSFPERPDMKPVVLKEQPFSLQRETSMNRPFVMLLTFNFSDGCGCSS
Sbjct: 301 GLRAPLPFLRSVEVSFPERPDMKPVVLKEQPFSLQRETSMNRPFVMLLTFNFSDGCGCSS 360

Query: 361 SSIEWPVDFLKQKDSFVRDRSLVLQELQHAAEHRSRAGQHAILEREGVPRAETSIHALVT 420
           SSIEWPVDFLKQKDSFVRDRSLVLQELQHAAEHRSRAGQHAILEREGVPRAETSIHALVT
Sbjct: 361 SSIEWPVDFLKQKDSFVRDRSLVLQELQHAAEHRSRAGQHAILEREGVPRAETSIHALVT 420

Query: 421 NIVRYDTEDSKAAVPMATWMNSNGSLSKRHMDAIGCNPASSKKQKLVATRHRRKGLNPAT 480
           NIVRYDTEDSKAAVPMATWMNSNGSLSKRHMDAIGCNPASSKKQKLVATRHRRKGLNPAT
Sbjct: 421 NIVRYDTEDSKAAVPMATWMNSNGSLSKRHMDAIGCNPASSKKQKLVATRHRRKGLNPAT 480

Query: 481 QKV 483
           QKV
Sbjct: 481 QKV 483



>Os12g0179800 Silent information regulator protein Sir2 family protein
          Length = 393

 Score =  101 bits (252), Expect = 1e-21,   Method: Compositional matrix adjust.
 Identities = 78/295 (26%), Positives = 129/295 (43%), Gaps = 60/295 (20%)

Query: 32  KKIEELAVMVRESKHLVVFTGAGISTSSGIPDFRGPKGVWT-----------------LQ 74
           K ++ L   + +SK L+V TGAG+ST SGIPD+R P G ++                  +
Sbjct: 99  KDVDLLYRFIDQSKKLMVLTGAGMSTESGIPDYRSPNGAYSSGFKPLTHQEFVRSIRARR 158

Query: 75  RSGKGVPGASLPFHRAVPTLTHMALVELEKTGRLKFVISQNVDSLHLRSGLPREKLAELH 134
           R           F RA P   H AL  LE+ GR+  +++QNVD LH R+G    K  ELH
Sbjct: 159 RYWARSYAGWRRFRRAQPNSAHYALASLERIGRVHSMVTQNVDRLHHRAG---SKPVELH 215

Query: 135 GNSFKEICPSCK--------KEYLRD-----------FEI------ETIGLKDTPRRC-- 167
           G+ ++  C  C         +E ++D            E+      ++ G++  P     
Sbjct: 216 GSVYEVACLDCGTSIDRESFQEQVKDLNPKWALAIDSLEVGQPGSDKSFGMQQRPDGDIE 275

Query: 168 -------------SDKNCGARLKDTVLDWEDALPPEEMDAAKEQCQKADLVLCLGTSLQI 214
                        S   CG  LK  V+ + D +P E  ++ KE  +  D +L +G++L  
Sbjct: 276 IDEKFWEQDFDIPSCNQCGGVLKPDVVMFGDNVPEERAESTKEAARNCDALLVVGSALMT 335

Query: 215 TPACNMPLLSLKNGGRVAIVNLQATPKDKKASLVIHGLVDKVIAGVMYMMNLRIP 269
             A  +  L+ +    +A + +  T  D   SL I+    +++  ++ M +L +P
Sbjct: 336 MSAFRLARLAHEANAPIAAITIGETRADSILSLKINARCGEILPRILQMGSLAVP 390



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.134    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 16,910,344
Number of extensions: 710281
Number of successful extensions: 1684
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 1683
Number of HSP's successfully gapped: 2
Length of query: 483
Length of database: 17,035,801
Length adjustment: 105
Effective length of query: 378
Effective length of database: 11,553,331
Effective search space: 4367159118
Effective search space used: 4367159118
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 158 (65.5 bits)