BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0131900 Os04g0131900|AK064088
         (609 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0131900  Similar to UDP-glucose:sterol glucosyltransfer...  1100   0.0  
Os01g0867600  Similar to UDP-glucose:sterol glucosyltransfer...   565   e-161
Os04g0131850  Conserved hypothetical protein                      526   e-149
Os07g0419500  Glycosyl transferase, family 28 domain contain...    78   2e-14

>Os04g0131900 Similar to UDP-glucose:sterol glucosyltransferase (EC 2.4.1.173)
          Length = 609

 Score = 1100 bits (2844), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 538/564 (95%), Positives = 538/564 (95%)

Query: 46  DDRGLPRSSTMPGGINNVEITNETAGPSNLERSRTERRRQNNPADDPTKQLFDDKISLKK 105
           DDRGLPRSSTMPGGINNVEITNETAGPSNLERSRTERRRQNNPADDPTKQLFDDKISLKK
Sbjct: 46  DDRGLPRSSTMPGGINNVEITNETAGPSNLERSRTERRRQNNPADDPTKQLFDDKISLKK 105

Query: 106 KLEMLNRIAXXXXXXXXXXXXPSSLETSTTDGVAYDGYSDVTVEEPLDGADISVRPPMDI 165
           KLEMLNRIA            PSSLETSTTDGVAYDGYSDVTVEEPLDGADISVRPPMDI
Sbjct: 106 KLEMLNRIATVKDDGTVVVDVPSSLETSTTDGVAYDGYSDVTVEEPLDGADISVRPPMDI 165

Query: 166 VILIVGTRGDVQPFIAIGKRLQDYGHRVRLATHANFKEFVLTAGLEFFPLGGDPKILAEY 225
           VILIVGTRGDVQPFIAIGKRLQDYGHRVRLATHANFKEFVLTAGLEFFPLGGDPKILAEY
Sbjct: 166 VILIVGTRGDVQPFIAIGKRLQDYGHRVRLATHANFKEFVLTAGLEFFPLGGDPKILAEY 225

Query: 226 MVKNKGFLPSGPSEIPIQRKQMKEIIFSLLPACKEPDPDTGIPFKVDAIIANPPAYGHTH 285
           MVKNKGFLPSGPSEIPIQRKQMKEIIFSLLPACKEPDPDTGIPFKVDAIIANPPAYGHTH
Sbjct: 226 MVKNKGFLPSGPSEIPIQRKQMKEIIFSLLPACKEPDPDTGIPFKVDAIIANPPAYGHTH 285

Query: 286 VAEALKVPIHIFFTMPWTPTSEFPHPLSRVKQAAGYRLSYQIVDSMIWLGIRDMINEFRK 345
           VAEALKVPIHIFFTMPWTPTSEFPHPLSRVKQAAGYRLSYQIVDSMIWLGIRDMINEFRK
Sbjct: 286 VAEALKVPIHIFFTMPWTPTSEFPHPLSRVKQAAGYRLSYQIVDSMIWLGIRDMINEFRK 345

Query: 346 KKLKLRPVTYLSGAQGSGNDIPHGYIWSPHLVPKPKDWGPKIDVVGFCFLDLASNYVPPE 405
           KKLKLRPVTYLSGAQGSGNDIPHGYIWSPHLVPKPKDWGPKIDVVGFCFLDLASNYVPPE
Sbjct: 346 KKLKLRPVTYLSGAQGSGNDIPHGYIWSPHLVPKPKDWGPKIDVVGFCFLDLASNYVPPE 405

Query: 406 PLVKWLEAGDKPIYVGFGSLPVQDPAKMTEVIVKALEITGQRGIINKGWGGLGTLAEPKD 465
           PLVKWLEAGDKPIYVGFGSLPVQDPAKMTEVIVKALEITGQRGIINKGWGGLGTLAEPKD
Sbjct: 406 PLVKWLEAGDKPIYVGFGSLPVQDPAKMTEVIVKALEITGQRGIINKGWGGLGTLAEPKD 465

Query: 466 FVYLLDNCPHDWLFLQCKXXXXXXXXXXXXXXLKAACPTTIVPFFGDQPFWGDRVHARGV 525
           FVYLLDNCPHDWLFLQCK              LKAACPTTIVPFFGDQPFWGDRVHARGV
Sbjct: 466 FVYLLDNCPHDWLFLQCKAVVHHGGAGTTAAGLKAACPTTIVPFFGDQPFWGDRVHARGV 525

Query: 526 GPLPIPVDQFSLQKLVDAINFMMEPKVKDNAVELAKAMESEDGVSGAVRAFLRHLPSRAE 585
           GPLPIPVDQFSLQKLVDAINFMMEPKVKDNAVELAKAMESEDGVSGAVRAFLRHLPSRAE
Sbjct: 526 GPLPIPVDQFSLQKLVDAINFMMEPKVKDNAVELAKAMESEDGVSGAVRAFLRHLPSRAE 585

Query: 586 ETAPQQTSSFLEFLGPVSKCLGCS 609
           ETAPQQTSSFLEFLGPVSKCLGCS
Sbjct: 586 ETAPQQTSSFLEFLGPVSKCLGCS 609



>Os01g0867600 Similar to UDP-glucose:sterol glucosyltransferase (EC 2.4.1.173)
          Length = 620

 Score =  565 bits (1455), Expect = e-161,   Method: Compositional matrix adjust.
 Identities = 258/419 (61%), Positives = 328/419 (78%), Gaps = 1/419 (0%)

Query: 161 PPMDIVILIVGTRGDVQPFIAIGKRLQDYGHRVRLATHANFKEFVLTAGLEFFPLGGDPK 220
           P + IVIL+VGTRGDVQPFIA+ KRLQ++GH VRLATHANF+ FV +AG++F+PLGGDP+
Sbjct: 165 PKLKIVILVVGTRGDVQPFIALAKRLQEFGHYVRLATHANFRTFVKSAGIDFYPLGGDPR 224

Query: 221 ILAEYMVKNKGFLPSGPSEIPIQRKQMKEIIFSLLPACKEPDPDTGIPFKVDAIIANPPA 280
           ILA+YM KNKGF  +GP+EI +QRKQ+KEIIFS+LPAC EPD DTG+PF+  AIIANPPA
Sbjct: 225 ILAQYMTKNKGFCLAGPTEISVQRKQLKEIIFSVLPACTEPDLDTGLPFRAQAIIANPPA 284

Query: 281 YGHTHVAEALKVPIHIFFTMPWTPTSEFPHPLSRVKQAAGYRLSYQIVDSMIWLGIRDMI 340
            GH H+AEAL VP+HIFFT PWTPT+EFPHPL+R+ Q+A YRLSY I+D +IW G R  I
Sbjct: 285 LGHLHIAEALGVPLHIFFTFPWTPTNEFPHPLARMPQSATYRLSYLILDLVIWWGTRGFI 344

Query: 341 NEFRKKKLKLRPVTYLSGAQGSGNDIPHGYIWSPHLVPKPKDWGPKIDVVGFCFLDLASN 400
           NEFR KKLKL P+ Y S   GS + +P GY+WSPHL+PKP DWGP +DVVG+CFL+L + 
Sbjct: 345 NEFR-KKLKLAPIAYFSTYHGSISHLPTGYMWSPHLMPKPNDWGPLVDVVGYCFLNLGTK 403

Query: 401 YVPPEPLVKWLEAGDKPIYVGFGSLPVQDPAKMTEVIVKALEITGQRGIINKGWGGLGTL 460
           Y PP+ L +WL+ G KPIY+GFGS+P+ D  K+T VI+ AL  TGQRGII++GWG LG+ 
Sbjct: 404 YQPPQELSQWLQQGPKPIYIGFGSMPLGDEKKVTSVILDALRETGQRGIISRGWGDLGSF 463

Query: 461 AEPKDFVYLLDNCPHDWLFLQCKXXXXXXXXXXXXXXLKAACPTTIVPFFGDQPFWGDRV 520
           +E    V++L++CPHDWLF +C               L A CPTTIVPFFGDQ FWG+R+
Sbjct: 464 SEVPVDVFILEDCPHDWLFPRCAAVVHHGGAGTTAAGLVAGCPTTIVPFFGDQFFWGERI 523

Query: 521 HARGVGPLPIPVDQFSLQKLVDAINFMMEPKVKDNAVELAKAMESEDGVSGAVRAFLRH 579
           HA+GVGP PIP+ + +++ L +AI FM++P+VK   +ELA A+ +EDGV+ AV +F RH
Sbjct: 524 HAQGVGPAPIPIAELTVEALSNAIRFMLDPEVKSRTMELAIAIGNEDGVAAAVDSFHRH 582



>Os04g0131850 Conserved hypothetical protein
          Length = 354

 Score =  526 bits (1355), Expect = e-149,   Method: Compositional matrix adjust.
 Identities = 258/292 (88%), Positives = 262/292 (89%), Gaps = 9/292 (3%)

Query: 321 YRLSYQIVDSMIWLGIRDMINEFRKKKLKLRPVTYLSGAQGSGNDIPHGYIWSPHLVPKP 380
           + LSYQIVDSMIWLGIRDMINEFRKKKLKLRPVTYLSGAQGSGNDIPHGYIWSPHLVPKP
Sbjct: 19  FVLSYQIVDSMIWLGIRDMINEFRKKKLKLRPVTYLSGAQGSGNDIPHGYIWSPHLVPKP 78

Query: 381 KDWGPKIDVVGFCFLDLASNYVPPEPLVKWLEAGDKPIYVGFGSLPVQDPAKMTEVIVKA 440
           KDWG KIDVVGFCFLDLASNYVPPEPL+KWLEAGDKPIYVGFGSLPVQDPAKMTEVIVKA
Sbjct: 79  KDWGLKIDVVGFCFLDLASNYVPPEPLIKWLEAGDKPIYVGFGSLPVQDPAKMTEVIVKA 138

Query: 441 LEITGQRGIINKGWGGLGTLAEPKDFVYLLDNCPHDWLFLQCKXXXXXXXXX-------- 492
           LEITGQRGIINKGWGGLGTLAEPKDFVYLLDNCPHDWLFLQCK                 
Sbjct: 139 LEITGQRGIINKGWGGLGTLAEPKDFVYLLDNCPHDWLFLQCKAVLLIMSSFHGVHHGGA 198

Query: 493 -XXXXXLKAACPTTIVPFFGDQPFWGDRVHARGVGPLPIPVDQFSLQKLVDAINFMMEPK 551
                 LKAACPTTIVPFFGDQPFWGDRVHARGVGPLPIPVDQFSL+KLVDAINFMMEPK
Sbjct: 199 GTTAAGLKAACPTTIVPFFGDQPFWGDRVHARGVGPLPIPVDQFSLRKLVDAINFMMEPK 258

Query: 552 VKDNAVELAKAMESEDGVSGAVRAFLRHLPSRAEETAPQQTSSFLEFLGPVS 603
           VK+ AVELAKAMESEDGVSG VRAFLRHLP RAEET PQ TSSFLEFLGPVS
Sbjct: 259 VKEKAVELAKAMESEDGVSGVVRAFLRHLPLRAEETTPQPTSSFLEFLGPVS 310



>Os07g0419500 Glycosyl transferase, family 28 domain containing protein
          Length = 60

 Score = 78.2 bits (191), Expect = 2e-14,   Method: Composition-based stats.
 Identities = 34/42 (80%), Positives = 39/42 (92%)

Query: 186 LQDYGHRVRLATHANFKEFVLTAGLEFFPLGGDPKILAEYMV 227
           LQ YGHRVRLATHANFK+FV+TAGLEF+PLGGDPK+LA  M+
Sbjct: 5   LQIYGHRVRLATHANFKDFVVTAGLEFYPLGGDPKLLAGCML 46



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.139    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 20,548,795
Number of extensions: 903030
Number of successful extensions: 1723
Number of sequences better than 1.0e-10: 4
Number of HSP's gapped: 1722
Number of HSP's successfully gapped: 4
Length of query: 609
Length of database: 17,035,801
Length adjustment: 107
Effective length of query: 502
Effective length of database: 11,448,903
Effective search space: 5747349306
Effective search space used: 5747349306
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 159 (65.9 bits)