BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os04g0116200 Os04g0116200|AK064677
         (299 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os04g0116200  Protein of unknown function DUF827, plant fami...   472   e-133
Os04g0116900  Protein of unknown function DUF827, plant fami...   257   9e-69

>Os04g0116200 Protein of unknown function DUF827, plant family protein
          Length = 299

 Score =  472 bits (1215), Expect = e-133,   Method: Compositional matrix adjust.
 Identities = 241/282 (85%), Positives = 241/282 (85%)

Query: 18  RAEIDMRAPFRSVKEAVVLFGEKETPLLAGDGDVHGPGLHRLRLMNRAATTPPAIWPTFX 77
           RAEIDMRAPFRSVKEAVVLFGEKETPLLAGDGDVHGPGLHRLRLMNRAATTPPAIWPTF 
Sbjct: 18  RAEIDMRAPFRSVKEAVVLFGEKETPLLAGDGDVHGPGLHRLRLMNRAATTPPAIWPTFS 77

Query: 78  XXXXXXXXXAVTRHVPXXXXXXXXXXXXXXXXKQKMAGCILSLQEELSNAMSELNKIKXX 137
                    AVTRHVP                KQKMAGCILSLQEELSNAMSELNKIK  
Sbjct: 78  QQQVSGGVVAVTRHVPAELEEARQELEKERSEKQKMAGCILSLQEELSNAMSELNKIKAR 137

Query: 138 XXXXXXXXXXXXXKVIDLQVEDLKFVEIDDDKPQPRQQSPSPGEFQKRRYVTFADPPPPM 197
                        KVIDLQVEDLKFVEIDDDKPQPRQQSPSPGEFQKRRYVTFADPPPPM
Sbjct: 138 DDVGDGDGGEAAAKVIDLQVEDLKFVEIDDDKPQPRQQSPSPGEFQKRRYVTFADPPPPM 197

Query: 198 ASAYDRAPPQAPLPDVVIELRHRHNHSSTPSPPQLREVRFMRQMSASHGMMKAAAVAAVD 257
           ASAYDRAPPQAPLPDVVIELRHRHNHSSTPSPPQLREVRFMRQMSASHGMMKAAAVAAVD
Sbjct: 198 ASAYDRAPPQAPLPDVVIELRHRHNHSSTPSPPQLREVRFMRQMSASHGMMKAAAVAAVD 257

Query: 258 QEGRKKKKSLIPLVGALFMRKKKSSSCSCHNDDSALNPRTSF 299
           QEGRKKKKSLIPLVGALFMRKKKSSSCSCHNDDSALNPRTSF
Sbjct: 258 QEGRKKKKSLIPLVGALFMRKKKSSSCSCHNDDSALNPRTSF 299



>Os04g0116900 Protein of unknown function DUF827, plant family protein
          Length = 307

 Score =  257 bits (656), Expect = 9e-69,   Method: Compositional matrix adjust.
 Identities = 176/311 (56%), Positives = 189/311 (60%), Gaps = 44/311 (14%)

Query: 18  RAEIDMRAPFRSVKEAVVLFGEKETPLLAGDGDVHGPGLHRLRLM------NRAATTPPA 71
           RAEID RAPFRSVKEAVVLFGEK   +LAG+  +H     RL         NRA TTPPA
Sbjct: 12  RAEIDTRAPFRSVKEAVVLFGEK---VLAGE--LHAGAGRRLLATSDQLQQNRATTTPPA 66

Query: 72  IWPTFXXXXX----XXXXXAVTRHVPXXXXXXXXXXXXX----XXXKQKMAGCILSLQEE 123
            WPT                 TRH P                    K+KMAGCILSLQEE
Sbjct: 67  RWPTVSQVKQIAGAVAAAAGATRHAPAMATAELEEAKQELEKERSEKKKMAGCILSLQEE 126

Query: 124 LSNAMSELNKIKXXXXXXXXXXXXXXXKVIDLQVEDLKFVEIDDDKPQPRQQSP------ 177
           LSNAMSELNK+K               KVIDLQVEDLKFVEIDDDKPQPR+QSP      
Sbjct: 127 LSNAMSELNKLKARDNEDDDGGGEAAAKVIDLQVEDLKFVEIDDDKPQPRRQSPSTTVTA 186

Query: 178 --------SPGEFQKRRYVTFADPPPPMASAYDRAPPQAPLPDVVIELRHRHNHSSTPSP 229
                   SPGEFQKRRYVTFADPP   ++AY RAP   PLPDVV+   H H  +   +P
Sbjct: 187 AAAAAGSASPGEFQKRRYVTFADPPTVASAAY-RAP---PLPDVVVMEPHHHRPA---AP 239

Query: 230 PQLREVRFMRQMSASHGMMKAAAVAAVDQEGRKKKKS-LIPLVGALFMRKKKSSSCSCHN 288
           P  REVRF RQMSA H  +K AA A   QE RKKKK  LIPLVGALFMRKKKSSS SCH 
Sbjct: 240 PLYREVRFQRQMSAGHEAVKVAAAAE--QEARKKKKKPLIPLVGALFMRKKKSSSRSCH- 296

Query: 289 DDSALNPRTSF 299
           DDSALN RT+F
Sbjct: 297 DDSALNSRTAF 307



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.318    0.132    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 9,792,910
Number of extensions: 456716
Number of successful extensions: 2961
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 2952
Number of HSP's successfully gapped: 2
Length of query: 299
Length of database: 17,035,801
Length adjustment: 100
Effective length of query: 199
Effective length of database: 11,814,401
Effective search space: 2351065799
Effective search space used: 2351065799
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 156 (64.7 bits)