BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0824000 Os03g0824000|AK111129
         (95 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0824000  Conserved hypothetical protein                      119   5e-28
Os03g0435900  Conserved hypothetical protein                      114   1e-26
Os03g0435800                                                       71   2e-13

>Os03g0824000 Conserved hypothetical protein
          Length = 95

 Score =  119 bits (298), Expect = 5e-28,   Method: Compositional matrix adjust.
 Identities = 66/95 (69%), Positives = 66/95 (69%)

Query: 1  MEAESAAARQAKESLELAFQMSQILDTGLDRHTLSLLMALCDRGANPEALAALVRELXXX 60
          MEAESAAARQAKESLELAFQMSQILDTGLDRHTLSLLMALCDRGANPEALAALVREL   
Sbjct: 1  MEAESAAARQAKESLELAFQMSQILDTGLDRHTLSLLMALCDRGANPEALAALVRELSSA 60

Query: 61 XXXXXXXXXXXXXXXXXXXXXXXXXXLFPSGLRKP 95
                                    LFPSGLRKP
Sbjct: 61 APPTTAAAAPSPASNATAAPSAKAASLFPSGLRKP 95



>Os03g0435900 Conserved hypothetical protein
          Length = 99

 Score =  114 bits (286), Expect = 1e-26,   Method: Compositional matrix adjust.
 Identities = 62/92 (67%), Positives = 63/92 (68%), Gaps = 3/92 (3%)

Query: 4  ESAAARQAKESLELAFQMSQILDTGLDRHTLSLLMALCDRGANPEALAALVRELXXXXXX 63
          ESAAARQAKESLELAFQMSQILDTGLDRHTLSLLMALCDRGANPEALAALVREL      
Sbjct: 11 ESAAARQAKESLELAFQMSQILDTGLDRHTLSLLMALCDRGANPEALAALVREL---SSA 67

Query: 64 XXXXXXXXXXXXXXXXXXXXXXXLFPSGLRKP 95
                                 LFPSGLR+P
Sbjct: 68 APPTAATTPASTATAVPSTKAASLFPSGLRQP 99



>Os03g0435800 
          Length = 186

 Score = 70.9 bits (172), Expect = 2e-13,   Method: Compositional matrix adjust.
 Identities = 35/43 (81%), Positives = 35/43 (81%)

Query: 1  MEAESAAARQAKESLELAFQMSQILDTGLDRHTLSLLMALCDR 43
          ME ES  AR AKESLELAF  SQILDT  DRHTLSLLMALCDR
Sbjct: 4  MEMESVVARHAKESLELAFWKSQILDTSCDRHTLSLLMALCDR 46



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.319    0.131    0.353 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 1,762,139
Number of extensions: 35877
Number of successful extensions: 125
Number of sequences better than 1.0e-10: 3
Number of HSP's gapped: 124
Number of HSP's successfully gapped: 3
Length of query: 95
Length of database: 17,035,801
Length adjustment: 64
Effective length of query: 31
Effective length of database: 13,694,105
Effective search space: 424517255
Effective search space used: 424517255
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 149 (62.0 bits)