BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0757400 Os03g0757400|AK105674
         (148 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0757400  Conserved hypothetical protein                      251   2e-67
Os10g0331500  Conserved hypothetical protein                       63   7e-11

>Os03g0757400 Conserved hypothetical protein
          Length = 148

 Score =  251 bits (640), Expect = 2e-67,   Method: Compositional matrix adjust.
 Identities = 130/148 (87%), Positives = 130/148 (87%)

Query: 1   MGGPSLCYACACRMLPLCCPRXXXXXXXXXXXXXXXXXXGDNPSLIRLELHSSPPHIAYV 60
           MGGPSLCYACACRMLPLCCPR                  GDNPSLIRLELHSSPPHIAYV
Sbjct: 1   MGGPSLCYACACRMLPLCCPRLDLLVVNQLLDLVLDLLLGDNPSLIRLELHSSPPHIAYV 60

Query: 61  ALVAVLVGEVRPAQKRHTMPEPFHRRVPPAVRHEACDRRVREHALLGSPTDDHSLAADTV 120
           ALVAVLVGEVRPAQKRHTMPEPFHRRVPPAVRHEACDRRVREHALLGSPTDDHSLAADTV
Sbjct: 61  ALVAVLVGEVRPAQKRHTMPEPFHRRVPPAVRHEACDRRVREHALLGSPTDDHSLAADTV 120

Query: 121 PELLQPAFAGTIGEVLPDHPQEWPARKH 148
           PELLQPAFAGTIGEVLPDHPQEWPARKH
Sbjct: 121 PELLQPAFAGTIGEVLPDHPQEWPARKH 148



>Os10g0331500 Conserved hypothetical protein
          Length = 286

 Score = 63.2 bits (152), Expect = 7e-11,   Method: Compositional matrix adjust.
 Identities = 43/97 (44%), Positives = 54/97 (55%), Gaps = 6/97 (6%)

Query: 55  PHIAYVALVAVLVGEVRPAQKRHTMPEPFHRRVPPAVRHEACDRRVREHALLGSPTDDHS 114
           P +A V  V  LVGEV P  +RH +P+  H RVPPAVR+EA  RRV +H LL  P  D +
Sbjct: 56  PDVADVRPVDELVGEVGPRHERHAVPDALHDRVPPAVRYEARHRRVSQHPLLRRPRHDLA 115

Query: 115 LAA------DTVPELLQPAFAGTIGEVLPDHPQEWPA 145
            AA      D V E ++      + +V   HPQE PA
Sbjct: 116 TAAVIAGGDDAVQEAVRVLVRVAMDDVWAHHPQERPA 152



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.323    0.139    0.459 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 5,918,838
Number of extensions: 249111
Number of successful extensions: 613
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 612
Number of HSP's successfully gapped: 2
Length of query: 148
Length of database: 17,035,801
Length adjustment: 92
Effective length of query: 56
Effective length of database: 12,232,113
Effective search space: 684998328
Effective search space used: 684998328
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 151 (62.8 bits)