BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0747400 Os03g0747400|Os03g0747400
         (257 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0747400  Seed maturation protein domain containing protein   406   e-114
Os06g0341300  Seed maturation protein domain containing protein   109   2e-24
Os03g0159600  Similar to Rab28 protein                             89   2e-18
Os09g0463400                                                       67   9e-12

>Os03g0747400 Seed maturation protein domain containing protein
          Length = 257

 Score =  406 bits (1044), Expect = e-114,   Method: Compositional matrix adjust.
 Identities = 219/257 (85%), Positives = 219/257 (85%)

Query: 1   MSQGQPRRPPPDGSGARPVMYGDVFDVSGELAGQPVAPRDAAKLQSAEEAVLGEAQKGGP 60
           MSQGQPRRPPPDGSGARPVMYGDVFDVSGELAGQPVAPRDAAKLQSAEEAVLGEAQKGGP
Sbjct: 1   MSQGQPRRPPPDGSGARPVMYGDVFDVSGELAGQPVAPRDAAKLQSAEEAVLGEAQKGGP 60

Query: 61  XXXXXXXXXXXXXXGHVGRAQVTGAIADEGVAVAVAETELPGRRVVTESVAGQVVARLAT 120
                         GHVGRAQVTGAIADEGVAVAVAETELPGRRVVTESVAGQVVARLAT
Sbjct: 61  AAAMQSAAAINARAGHVGRAQVTGAIADEGVAVAVAETELPGRRVVTESVAGQVVARLAT 120

Query: 121 PPRVVATQPSGSLDKDAVTIGRALEWXXXXXXXXAAEMLATGRTVTVPGGVXXXXXXXXX 180
           PPRVVATQPSGSLDKDAVTIGRALEW        AAEMLATGRTVTVPGGV         
Sbjct: 121 PPRVVATQPSGSLDKDAVTIGRALEWRPRPPRRRAAEMLATGRTVTVPGGVAAAAQAAAD 180

Query: 181 XXXXXXXRDEDRIKLRDVLTGARGKLPADKAATREDAERVPSAEVRNRPDMATTPGGVAD 240
                  RDEDRIKLRDVLTGARGKLPADKAATREDAERVPSAEVRNRPDMATTPGGVAD
Sbjct: 181 HNAAPAARDEDRIKLRDVLTGARGKLPADKAATREDAERVPSAEVRNRPDMATTPGGVAD 240

Query: 241 AVTAAARLNQERPTRSF 257
           AVTAAARLNQERPTRSF
Sbjct: 241 AVTAAARLNQERPTRSF 257



>Os06g0341300 Seed maturation protein domain containing protein
          Length = 277

 Score =  109 bits (273), Expect = 2e-24,   Method: Compositional matrix adjust.
 Identities = 94/249 (37%), Positives = 122/249 (48%), Gaps = 26/249 (10%)

Query: 19  VMYGDVFDVSGELAGQPVAPRDAAKLQSAEEAVLG----EAQKGGPXX--XXXXXXXXXX 72
           + YG VF V+GELAGQP+APRDAA ++SAEE+V G    +   GG               
Sbjct: 28  IKYGHVFAVTGELAGQPIAPRDAAAMRSAEESVPGVPVPQDIGGGYSAGVAMETAAAYNQ 87

Query: 73  XXGHVGRAQVTGAIADEGVAVAVAETELPGRRVVTESVAGQVVARLATPPRVVATQPSGS 132
               V   Q + A  ++G+AV   +T +PG R++TE VAGQVV + +   + V       
Sbjct: 88  AVAAVRPGQASDAATNQGIAVT--QTSVPGGRIITEFVAGQVVGQYSVADQAV------E 139

Query: 133 LDKDAVTIGRALEWX-----------XXXXXXXAAEMLATGRTVTVPGGVXXXXXXXXXX 181
            D   +T+G ALE                    AAEM A G  VT+PGG+          
Sbjct: 140 QDATKITVGEALEATALAAGERPVDRTDVEAIRAAEMAAQGSDVTMPGGLADQAQAAARS 199

Query: 182 XXXXXXRDEDRIKLRDVLTGARGKLPADKAATREDAERVPSAEVRNRPDMATTPGGVADA 241
                 RD+D+I + DVLT A  KL  DK A  EDA +V  AE  +     T  GGV  A
Sbjct: 200 NADAD-RDDDKITVGDVLTDATAKLAGDKVAGAEDAAKVVQAETYSDAAARTRAGGVGAA 258

Query: 242 VTAAARLNQ 250
           V+ AARLNQ
Sbjct: 259 VSTAARLNQ 267



>Os03g0159600 Similar to Rab28 protein
          Length = 276

 Score = 89.4 bits (220), Expect = 2e-18,   Method: Compositional matrix adjust.
 Identities = 58/123 (47%), Positives = 72/123 (58%), Gaps = 7/123 (5%)

Query: 1   MSQGQPRRPPPDGSGA----RPVMYGDVFDVSGELAGQPVAPRDAAKLQSAEEAVLGEAQ 56
           MSQ QPRRP    +G     + + YGDVF V+G LA +P+APRDAA +QSAE  VLG+  
Sbjct: 1   MSQEQPRRPSEKAAGGGGGEQGIRYGDVFPVTGSLAAKPIAPRDAATMQSAENLVLGKTV 60

Query: 57  KGGPXXXXXXXXXXXXXXGHVGRAQVTGAIADEGVAVAVAETELP-GRRVVTESVAGQVV 115
           KGGP              G VG  Q T A A++G  V V++T +P G R+VTE VAGQ V
Sbjct: 61  KGGPAAAMESAASRNEEMGVVGHDQATDAAAEQG--VNVSDTLVPGGGRIVTEFVAGQAV 118

Query: 116 ARL 118
              
Sbjct: 119 GHY 121



>Os09g0463400 
          Length = 99

 Score = 67.4 bits (163), Expect = 9e-12,   Method: Compositional matrix adjust.
 Identities = 39/93 (41%), Positives = 53/93 (56%), Gaps = 3/93 (3%)

Query: 19  VMYGDVFDVSGELAGQPVAPRDAAKLQSAEEAVLGEAQKGGPXXXXXXXXXXXXXXGHVG 78
           + YGDVF ++  LA +P+ P DAA +QSAE  VLG+  KGGP              G +G
Sbjct: 6   IWYGDVFPLTRSLAAKPIVPWDAATMQSAENLVLGKTVKGGPAAAMESATSRNEEMGVIG 65

Query: 79  RAQVTGAIADEGVAVAVAETELP-GRRVVTESV 110
             Q T A A+ G  V +++T +P G R+VTE V
Sbjct: 66  HDQSTDATAEHG--VNISDTLVPGGGRIVTEFV 96



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.312    0.130    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 8,222,842
Number of extensions: 316311
Number of successful extensions: 859
Number of sequences better than 1.0e-10: 5
Number of HSP's gapped: 849
Number of HSP's successfully gapped: 5
Length of query: 257
Length of database: 17,035,801
Length adjustment: 99
Effective length of query: 158
Effective length of database: 11,866,615
Effective search space: 1874925170
Effective search space used: 1874925170
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 155 (64.3 bits)