BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0640200 Os03g0640200|AY224578
         (113 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0640200  Conserved hypothetical protein                      237   2e-63
Os03g0640400  Conserved hypothetical protein                      192   3e-50

>Os03g0640200 Conserved hypothetical protein
          Length = 113

 Score =  237 bits (604), Expect = 2e-63,   Method: Compositional matrix adjust.
 Identities = 113/113 (100%), Positives = 113/113 (100%)

Query: 1   MEKSPLAWFRLLVNNEDVVAIKQMQHLILGRLQDSNAVLTHFNEYSEQCFAEVSNDFAGK 60
           MEKSPLAWFRLLVNNEDVVAIKQMQHLILGRLQDSNAVLTHFNEYSEQCFAEVSNDFAGK
Sbjct: 1   MEKSPLAWFRLLVNNEDVVAIKQMQHLILGRLQDSNAVLTHFNEYSEQCFAEVSNDFAGK 60

Query: 61  TRLLKSMKADLDHIFLKLRGMKSRLAATYPDAFPTGAMAETMDQRPDLESPLD 113
           TRLLKSMKADLDHIFLKLRGMKSRLAATYPDAFPTGAMAETMDQRPDLESPLD
Sbjct: 61  TRLLKSMKADLDHIFLKLRGMKSRLAATYPDAFPTGAMAETMDQRPDLESPLD 113



>Os03g0640400 Conserved hypothetical protein
          Length = 123

 Score =  192 bits (489), Expect = 3e-50,   Method: Compositional matrix adjust.
 Identities = 98/123 (79%), Positives = 102/123 (82%), Gaps = 10/123 (8%)

Query: 1   MEKSP----------LAWFRLLVNNEDVVAIKQMQHLILGRLQDSNAVLTHFNEYSEQCF 50
           MEKSP           A FR LV+  D+ AI+Q QHLILGRLQDSNAVLTHFNEYSEQCF
Sbjct: 1   MEKSPPETAAAAAEVAARFRSLVDTGDIGAIRQTQHLILGRLQDSNAVLTHFNEYSEQCF 60

Query: 51  AEVSNDFAGKTRLLKSMKADLDHIFLKLRGMKSRLAATYPDAFPTGAMAETMDQRPDLES 110
           AEVSNDFA KTRLLKSMK DLDHIFLKLR MKSRLAATYPDAFP GAMA+TMDQRPDLES
Sbjct: 61  AEVSNDFASKTRLLKSMKDDLDHIFLKLRSMKSRLAATYPDAFPDGAMAKTMDQRPDLES 120

Query: 111 PLD 113
           PLD
Sbjct: 121 PLD 123



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.320    0.133    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 3,397,408
Number of extensions: 108955
Number of successful extensions: 288
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 288
Number of HSP's successfully gapped: 2
Length of query: 113
Length of database: 17,035,801
Length adjustment: 80
Effective length of query: 33
Effective length of database: 12,858,681
Effective search space: 424336473
Effective search space used: 424336473
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 149 (62.0 bits)