BLASTP 2.2.23 [Feb-03-2010]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment:
Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala,
Aleksandr Morgulis, Alejandro A. Schäffer, and Yi-Kuo Yu (2005) "Protein database
searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= Os03g0584300 Os03g0584300|AK105133
         (123 letters)

Database: rap3 
           52,214 sequences; 17,035,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

Os03g0584300  Protein of unknown function UPF0136, Transmemb...   147   1e-36
Os03g0568500  Protein of unknown function UPF0136, Transmemb...    71   1e-13

>Os03g0584300 Protein of unknown function UPF0136, Transmembrane family protein
          Length = 123

 Score =  147 bits (372), Expect = 1e-36,   Method: Compositional matrix adjust.
 Identities = 82/123 (66%), Positives = 82/123 (66%)

Query: 1   MEDFYVTIPXXXXXXXXXXXXXXKRGSKXXXXXXXXXXXXXXXXXXXXXXXXXXXHPSGS 60
           MEDFYVTIP              KRGSK                           HPSGS
Sbjct: 1   MEDFYVTIPYGAAVLVGGVAGYLKRGSKASLAAGGGFGGALLLAGALSARAFARGHPSGS 60

Query: 61  LFAAVLQTVCAVALTVVMGIRYIKTRKVMPAGIIAAVSALVLIFYIYKISNGGNKVYVPV 120
           LFAAVLQTVCAVALTVVMGIRYIKTRKVMPAGIIAAVSALVLIFYIYKISNGGNKVYVPV
Sbjct: 61  LFAAVLQTVCAVALTVVMGIRYIKTRKVMPAGIIAAVSALVLIFYIYKISNGGNKVYVPV 120

Query: 121 SAE 123
           SAE
Sbjct: 121 SAE 123



>Os03g0568500 Protein of unknown function UPF0136, Transmembrane family protein
          Length = 119

 Score = 71.2 bits (173), Expect = 1e-13,   Method: Compositional matrix adjust.
 Identities = 31/57 (54%), Positives = 45/57 (78%)

Query: 60  SLFAAVLQTVCAVALTVVMGIRYIKTRKVMPAGIIAAVSALVLIFYIYKISNGGNKV 116
           S  A  L+T+CA+ALT VMG RY++T K+MPAG++A +SAL+  FY++KI+ GGN +
Sbjct: 58  SYLALALETLCALALTYVMGQRYLETSKIMPAGVVAGLSALMSAFYLFKIATGGNHI 114



  Database: rap3
    Posted date:  Nov 19, 2010  6:03 PM
  Number of letters in database: 17,035,801
  Number of sequences in database:  52,214
  
Lambda     K      H
   0.325    0.138    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 52214
Number of Hits to DB: 2,335,947
Number of extensions: 55905
Number of successful extensions: 153
Number of sequences better than 1.0e-10: 2
Number of HSP's gapped: 152
Number of HSP's successfully gapped: 2
Length of query: 123
Length of database: 17,035,801
Length adjustment: 88
Effective length of query: 35
Effective length of database: 12,440,969
Effective search space: 435433915
Effective search space used: 435433915
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 149 (62.0 bits)